Identification of microRNA transcriptome reveals that miR-100 is involved in the renewal of porcine intestinal epithelial cells

Zou, Lijun; Xiong, Xia; Yang, Huansheng; Wang, Kexing; Zhou, Jian; Lv, Dinghong; Yin, Yulong

doi:10.1007/s11427-018-9338-9

Cited by 17 publications

(15 citation statements)

References 35 publications

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“…Beta-actin is widely distributed in various tissues and cells of mammalian animals, where the amino acid sequence is highly conservative ( Nicot et al., 2005 ; Cornejo et al., 2010 ). The many intestine-related experiments we have previously conducted revealed that results are reliable when β-actin was used alone ( Zou et al., 2019 ). Additionally, pretests were done that showed β-actin alone can be used.…”

Section: Methodsmentioning

confidence: 99%

Effects of varying dietary folic acid during weaning stress of piglets

et al. 2021

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The present study was conducted to evaluate the effect of dietary folic acid on the growth performance, intestinal morphology, and intestinal epithelial cells renewal in post-weaning piglets. Twenty-eight piglets (weaned at day 21, initial body weight of 6.73 ± 0.62 kg) were randomly allotted to 4 treatments with 7 pens per diet and 1 piglet per pen. The piglets were fed the same antibiotic-free and zinc oxide-free basal diets supplemented with folic acid at 0, 3, 9, and 18 mg/kg for 14 days. The results showed that dietary supplementation with folic acid increased villus height (VH) ( P = 0.003; linear, P = 0.001), VH-to-crypt depth (VH:CD) ratio ( P = 0.002; linear, P = 0.001), villus surface area (VSA) ( P = 0.026; linear, P = 0.010). The analyzed parameters ADG, serum urea nitrogen (BUN) content, VH, VSA, and serum folate (SF) concentration responded linearly to the dietary folic acid concentration when the dietary folic acid concentration was below 4.42, 5.26, 4.79, 3.47, and 3.53 mg/kg respectively ( R 2 = 0.995, 0.995, 0.999, 0.999, 0.872, P = 0.09, 0.07, 0.09, 0.09, 0.36, respectively), as assessed by a two-linear broken-line regression. Above these breakpoints, the response of ADG, VH, VSA, and SF plateaued in response to changes in dietary folic acid concentration. Moreover, dietary supplementation with folic acid significantly increased the lactase ( P = 0.001; linear, P = 0.001) and sucrase activities ( P = 0.021; linear, P = 0.010) in the jejunal mucosa of weaned piglets. The mRNA expression of solute carrier family 6 member 19 ( SLC6a19 ), solute carrier family 1 member 1 ( SLC7a1 ), tumor necrosis factor-α ( TNF-α ), the number of Ki67 positive cells, and cell shedding rate had a significant linear contrast ( P = 0.023, 0.021, 0.038, 0.049, and 0.008, respectively) in dietary folic acid groups. In conclusion, our results indicate that folic acid supplementation can improve the growth performance and intestinal morphology of weaned piglets by maintaining the balance of epithelial cell renewal.

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Section: Methodsmentioning

confidence: 99%

Effects of varying dietary folic acid during weaning stress of piglets

et al. 2021

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“…The intestinal tract is an important interface between the host and its environment, playing an important role in the digestion and absorption of nutrients, tolerance to harmless and beneficial microorganisms, and appropriate immune responses, in addition to preventing the transfer of intestinal microorganisms to sterile sites ( Farhadi et al., 2003 ; Neu, 2007 ; Zou et al., 2019 ). From another point of view, IEC are a selective-permeability barrier that not only completes the absorption of nutrients and electrolytes but also effectively blocks the invasion of antigens such as bacteria and potential allergens ( Groschwitz and Hogan, 2009 ).…”

Section: Gut Immunity Of Monogastric-production Animalsmentioning

confidence: 99%

The impact of probiotics on gut health via alternation of immune status of monogastric animals

Ding

Yan

et al. 2021

Animal Nutrition

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The intestinal immune system is affected by various factors during its development, such as maternal antibodies, host genes, intestinal microbial composition and activity, and various stresses (such as weaning stress). Intestinal microbes may have an important impact on the development of the host immune system. Appropriate interventions such as probiotics may have a positive effect on intestinal immunity by regulating the composition and activity of intestinal microbes. Moreover, probiotics participate in the regulation of host health in many ways; for instance, by improving digestion and the absorption of nutrients, immune response, increasing the content of intestinal-beneficial microorganisms, and inhibiting intestinal-pathogenic bacteria, and they participate in regulating intestinal diseases in various ways. Probiotics are widely used as additives in livestock and the poultry industry and bring health benefits to hosts by improving intestinal microbes and growth performance, which provides more choices for promoting strong and efficient productivity.

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“…A key disadvantage of cell-free miRNA assessments is their low abundance [26], and miRNAs are associated with intracellular mRNA contamination, degradation, and low stability. Nevertheless, changes in circulating miR-NAs are indicative of aberrant cancer immunity, cell growth, stromal interaction, and proliferation, meaning that these miRNAs can be used for diagnosis and prognostication [25][26][27]. In this study, 10 PCa-specific miRNAs were quantified in PCa subjects' serum samples, and miR-141, miR-182, miR-200b, and miR-375 were higher in the PCa subjects compared to the BPH subjects.…”

Section: Discussionmentioning

confidence: 82%

Circulating miRNAs as Biomarkers for Prostate Cancer Diagnosis in Subjects with Benign Prostatic Hyperplasia

Jin

Fei

Wang

et al. 2020

Journal of Immunology Research

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Body fluids often contain freely circulating nucleic acids, many of which can be exploited as noninvasive tools for the diagnosis of cancer as well as for clinical prognostication. Identifying microRNAs (miRNAs) in subjects’ blood with various malignancies means that they can serve as novel biomarkers for prostate cancer (PCa) diagnosis. This study analyzed serum-circulating miRNAs as a noninvasive biomarker in subjects with PCa and subjects with benign prostatic hyperplasia (BPH). In total, 31 PCa subjects and 31 BPH subjects were included, with the BPH group serving as the control group. RT-qPCR was used to quantify the levels of 10 miRNAs, which included miR-18a, miR-34a, miR-106b, miR-183, miR-200a, miR-301a, miR-141, miR-182, miR-200b, and miR-375 in serum. Statistical tests were used to assess the relationship between the levels of miRNAs and the clinicopathological data. A significant increase was observed in the relative expression ratios of miR-141, miR-182, miR-200b, and miR-375 (1.89-, 2.09-, 2.41-, and 2.27-folds, respectively) in the PCa group when compared to the BPH group. Based on the receiver operating characteristic (ROC) analysis, the largest area under the curve (AUC), 0.923, was associated with the miR-200b group, indicating effective diagnostic properties for this biomarker. A correlation was observed between total prostate-specific antigen (TPSA) and the relative levels of miR-141, miR-182, miR-200b, and miR-375. The Gleason score and the miR-200b expression level were also correlated. These results are consistent with previous studies regarding the possibility of differentiating between PCa subjects and healthy controls based on the detection of miRNA. The findings attest to a distinctive expression profile of miRNA that is detectable in the blood of PCa subjects, thereby confirming the role of miRNAs as diagnostic biomarkers for PCa.

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Identification of microRNA transcriptome reveals that miR-100 is involved in the renewal of porcine intestinal epithelial cells

Cited by 17 publications

References 35 publications

Effects of varying dietary folic acid during weaning stress of piglets

Effects of varying dietary folic acid during weaning stress of piglets

The impact of probiotics on gut health via alternation of immune status of monogastric animals

Circulating miRNAs as Biomarkers for Prostate Cancer Diagnosis in Subjects with Benign Prostatic Hyperplasia

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