Identification of metabolic pathways of the lipid peroxidation product 4-hydroxynonenal in in situ perfused rat kidney

Diabetes impairs the enzymatic disposal of 4-hydroxynonenal in rat liver

“…The correlation of HNE consumption with GST and ALDH activity is consistent with the opinion that conjugation with GSH catalyzed by GST and ALDH-mediated oxidation are the main metabolic pathways of HNE removal [18,19,39].…”

Diabetes impairs the enzymatic disposal of 4-hydroxynonenal in rat liver

“…The correlation of HNE consumption with GST and ALDH activity is consistent with the opinion that conjugation with GSH catalyzed by GST and ALDH-mediated oxidation are the main metabolic pathways of HNE removal [18,19,39].…”

Lipoperoxidation in hepatic subcellular compartments of diabetic rats

“…However, the respective role of the various subcellular compartment in the HNE metabolism is not completely defined yet, and it probably depends on the cellular conditions [54]. Moreover, the relative role of each HNE metabolising enzyme has been determined by various Authors with different results [14,[55][56][57] and again the pattern could depend on the conditions [58]. GSH conjugation by glutathione S-transferase seems to play a major role.…”

Structural Impact of 4‐Hydroxynonenal Modification on Human Cytochrome CYP4F11

“…HPLC analysis of 4‐HNE [ 62 ] and separately 15‐HETE and 15,20‐dihydroxyeicosatetraenoic acid (15,20‐diHETE) was performed on an Agilent 1200 Series HPLC system (Agilent Technologies, Santa Clara, CA), equipped with a diode array detector (DAD) and a reverse‐phase column (Zorbax Eclipse Plus C18, 250 × 4.6 mm, 5 μm). A sample volume of 10 μL was injected, and the analysis was conducted at a controlled column temperature of +25°C.…”

Diabetes impairs the enzymatic disposal of 4-hydroxynonenal in rat liver

“…The correlation of HNE consumption with GST and ALDH activity is consistent with the opinion that conjugation with GSH catalyzed by GST and ALDH-mediated oxidation are the main metabolic pathways of HNE removal [18,19,39].…”

Lipoperoxidation in hepatic subcellular compartments of diabetic rats

“…However, the respective role of the various subcellular compartment in the HNE metabolism is not completely defined yet, and it probably depends on the cellular conditions [54]. Moreover, the relative role of each HNE metabolising enzyme has been determined by various Authors with different results [14,[55][56][57] and again the pattern could depend on the conditions [58]. GSH conjugation by glutathione S-transferase seems to play a major role.…”

Structural Impact of 4‐Hydroxynonenal Modification on Human Cytochrome CYP4F11

“…HPLC analysis of 4‐HNE [ 62 ] and separately 15‐HETE and 15,20‐dihydroxyeicosatetraenoic acid (15,20‐diHETE) was performed on an Agilent 1200 Series HPLC system (Agilent Technologies, Santa Clara, CA), equipped with a diode array detector (DAD) and a reverse‐phase column (Zorbax Eclipse Plus C18, 250 × 4.6 mm, 5 μm). A sample volume of 10 μL was injected, and the analysis was conducted at a controlled column temperature of +25°C.…”

Diabetes impairs the enzymatic disposal of 4-hydroxynonenal in rat liver

“…The correlation of HNE consumption with GST and ALDH activity is consistent with the opinion that conjugation with GSH catalyzed by GST and ALDH-mediated oxidation are the main metabolic pathways of HNE removal [18,19,39].…”

Lipoperoxidation in hepatic subcellular compartments of diabetic rats

“…However, the respective role of the various subcellular compartment in the HNE metabolism is not completely defined yet, and it probably depends on the cellular conditions [54]. Moreover, the relative role of each HNE metabolising enzyme has been determined by various Authors with different results [14,[55][56][57] and again the pattern could depend on the conditions [58]. GSH conjugation by glutathione S-transferase seems to play a major role.…”

Structural Impact of 4‐Hydroxynonenal Modification on Human Cytochrome CYP4F11

“…HPLC analysis of 4‐HNE [ 62 ] and separately 15‐HETE and 15,20‐dihydroxyeicosatetraenoic acid (15,20‐diHETE) was performed on an Agilent 1200 Series HPLC system (Agilent Technologies, Santa Clara, CA), equipped with a diode array detector (DAD) and a reverse‐phase column (Zorbax Eclipse Plus C18, 250 × 4.6 mm, 5 μm). A sample volume of 10 μL was injected, and the analysis was conducted at a controlled column temperature of +25°C.…”