Identification of Leishmania species by high-resolution DNA dissociation in cases of American cutaneous leishmaniasis

Veasey, John Verrinder; Zampieri, Ricardo Andrade; Lellis, Rute Facchini; Freitas, Thais Helena Proença de; Winter, Lucile Maria Floeter

doi:10.1016/j.abd.2020.02.003

Cited by 9 publications

References 47 publications

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“…The first was the capacity of Pintomyia ovallesi to harbor two different Leishmania species. These findings coincide with previous reports depicting the intimate relationship of this vector with the parasite ( 34 , 35 ), as well as the fundamental role that P. ovallesi plays in the transmission cycle of the parasite and progression across the clinical spectrum. Second, our results underscore the close relationship of Migonemyia migonei and Lutzomyia longipalpis with T. cruzi ( Fig.…”

Section: Discussionsupporting

confidence: 92%

Development of an Amplicon-Based Next-Generation Sequencing Protocol to Identify Leishmania Species and Other Trypanosomatids in Leishmaniasis Endemic Areas

et al. 2021

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Traditionally, there has been a frequent, yet incorrect assumption that phlebotomine vectors, animal reservoirs, and human hosts are susceptible to Leishmania infection by a single parasite species. However, current evidence supports that these new vector-parasite-reservoir associations lend vectors and reservoirs greater permissiveness to certain Leishmania species, thus promoting the appearance of coinfection events, particularly in disease-endemic regions.

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Section: Discussionsupporting

confidence: 92%

Development of an Amplicon-Based Next-Generation Sequencing Protocol to Identify Leishmania Species and Other Trypanosomatids in Leishmaniasis Endemic Areas

et al. 2021

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“…By using small amounts of biological material and distinguishing polymorphic differences in genetic material, different PCR-based techniques have been developed and employed for detection and identification of Leishmania (Veasey et al 2020). Several genes are used as target sequences for Leishmania-specific identification, such as the glucose-6-phosphate dehydrogenase enzyme gene (g6pd), ribosomal DNA (rDNA) and especially kinetoplast DNA (kDNA) (Reimão et al 2020;Veasey et al 2020). In blood samples from dogs, it is well established that kDNA has better sensitivity compared to nuclear DNA and, for this reason, has been widely used for the detection of parasite DNA in these animals (Lachaud et al 2002).…”

Section: Diagnostic Methods For Felmentioning

confidence: 99%

Epidemiological and diagnostic aspects of feline leishmaniasis with emphasis on Brazil: a narrative review

et al. 2021

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“…It is mainly observed on the face ( Bugssa et al, 2014a ; Bekele et al, 2014a ; Amin et al, 2013 ). Leishmania (L) major, L. tropica , L. aethiopica, L. amazonensis and L. braziliensis are the most common Leishmania species causing CL ( Sabzevari et al, 2021 ; Veasey et al, 2020 ; Steverding, 2017 ). Rarely, it can also be caused by leishmania donovani and L. infantum ( Salam et al, 2014 ; Khatri et al, 2016 ) .…”

Section: Introductionmentioning

confidence: 99%

Knowledge, attitude and prevention measures of students towards cutaneous leishmaniasis in Delanta district, Northeast Ethiopia

Dires

Kumar

Addis

et al. 2022

Parasite Epidemiology and Control

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Background and aims Leishmaniasis is a neglected tropical disease which causes significant morbidity and mortality, particularly in developing countries. Cutaneous leishmaniasis (CL) is the main form of leishmaniasis that affects the skin. Knowledge and perception of people about leishmaniasis has a great impact to prevent the disease in endemic areas. Hence, the aim of this study was to assess the knowledge, attitude and preventive measures of Wegeltena secondary school students towards CL in Delanta district, one of the endemic areas in Northeast Ethiopia. Methods A facility-based cross-sectional study was conducted from January 4 to 20, 2021 at Wegeltena secondary school in Delanta district, Northeast Ethiopia. A pre-tested, interviewer administered structured questionnaire was used to collect the data. Multivariable logistic regression analysis was utilized to measure the degree of association between outcome and independent variables. Statistically significant association with the outcome variables was declared at a p -value of <0.05. Results A total of 388 students were participated in the study. In overall, 27.6% and 34.5% of participants had good knowledge and favorable attitude towards CL, respectively. Being grade 12 (AOR = 2.56; 95% CI: 1.23–5.29) and dwelling in urban areas (AOR = 1.78; 95% CI: 1.09–2.89) were determinants of good knowledge. However, female sex (AOR = 1.96; 95% CI: 1.21–3.18) and had good knowledge about CL (AOR = 3.75; 95% CI: 2.26–6.21) were significantly associated with favorable attitude of respondents towards the disease. Conclusion In this study , nearly three-fourth of participants had poor knowledge about CL and two-third of them had unfavorable attitude towards the disease. Level of education and residence were determinants of respondents' knowledge about CL. Moreover, sex and level of knowledge about CL were determinants of respondents' attitude towards the disease. Therefore, an intensified health education program shall be implemented in schools that are found in endemic areas.

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Identification of Leishmania species by high-resolution DNA dissociation in cases of American cutaneous leishmaniasis

Cited by 9 publications

References 47 publications

Development of an Amplicon-Based Next-Generation Sequencing Protocol to Identify Leishmania Species and Other Trypanosomatids in Leishmaniasis Endemic Areas

Development of an Amplicon-Based Next-Generation Sequencing Protocol to Identify Leishmania Species and Other Trypanosomatids in Leishmaniasis Endemic Areas

Epidemiological and diagnostic aspects of feline leishmaniasis with emphasis on Brazil: a narrative review

Knowledge, attitude and prevention measures of students towards cutaneous leishmaniasis in Delanta district, Northeast Ethiopia

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