Identification of key bacterial players during successful full-scale soil field bioremediation in Antarctica

Álvarez, Lauro Vladimir Valle; Bolhuis, Henk; Goh, Kian Mau; Chan, Kok‐Gan; Sing, Chan Chia; Cormack, Walter Mac; Ruberto, Lucas

doi:10.1016/j.ibiod.2021.105354

Cited by 9 publications

(5 citation statements)

References 64 publications

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“…Moreover, to enhance phenanthrene degradation by synthetic consortia, some minor bacterial genera, such as Rhodococcus and Polaromonas , identified based on bacterial community data of the 16S rRNA gene amplicon results, should be targeted in future isolation attempts from enriched consortia. A common challenge in previous studies was that some bacteria could not be cultivated by the methods used; moreover, slow-growing synergistic partners disappeared during isolation and/or because of the presence of metabolic dependencies in microbial communities 54 , 55 .…”

Section: Resultsmentioning

confidence: 99%

Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures

Naloka,

Kuntaveesuk,

Muangchinda

et al. 2024

Sci Rep

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Hydrocarbon contamination, including contamination with polycyclic aromatic hydrocarbons (PAHs), is a major concern in Antarctica due to the toxicity, recalcitrance and persistence of these compounds. Under the Antarctic Treaty, nonindigenous species are not permitted for use in bioremediation at polluted sites in the Antarctic region. In this study, three bacterial consortia (C13, C15, and C23) were isolated from Antarctic soils for phenanthrene degradation. All isolated bacterial consortia demonstrated phenanthrene degradation percentages ranging from 45 to 85% for 50 mg/L phenanthrene at 15 ℃ within 5 days. Furthermore, consortium C13 exhibited efficient phenanthrene degradation potential across a wide range of environmental conditions, including different temperature (4–30 ℃) and water availability (without polyethylene glycol (PEG) 6000 or 30% PEG 6000 (w/v)) conditions. Sequencing analysis of 16S rRNA genes revealed that Pseudomonas and Pseudarthrobacter were the dominant genera in the phenanthrene-degrading consortia. Moreover, six cultivable strains were isolated from these consortia, comprising four strains of Pseudomonas, one strain of Pseudarthrobacter, and one strain of Paeniglutamicibacter. These isolated strains exhibited the ability to degrade 50 mg/L phenanthrene, with degradation percentages ranging from 4 to 22% at 15 ℃ within 15 days. Additionally, the constructed consortia containing Pseudomonas spp. and Pseudarthrobacter sp. exhibited more effective phenanthrene degradation (43–52%) than did the individual strains. These results provide evidence that Pseudomonas and Pseudarthrobacter can be potential candidates for synergistic phenanthrene degradation at low temperatures. Overall, our study offers valuable information for the bioremediation of PAH contamination in Antarctic environments.

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Section: Resultsmentioning

confidence: 99%

Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures

Naloka,

Kuntaveesuk,

Muangchinda

et al. 2024

Sci Rep

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“…By contrast, native microorganisms adapt well to the Antarctic environment. Therefore, the bioremediation of an oil-contaminated Antarctic environment by indigenous microbes represents a promising approach to mitigate the anthropogenic impact in Antarctica [5,6].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Hydrocarbon Degradation Potential of Variovorax sp. Strain N23 Isolated from the Antarctic Soil

Liu

Cui

Hao

et al. 2023

Microbiology Research

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Increasing pollution has significantly threatened the Antarctic ecosystem. The contamination of hydrocarbons has drawn a considerable amount of attention owing to their toxicity, recalcitrance, and persistence. Considering the Antarctic Treaty, only indigenous species are allowed to bioremediate the contaminated environment. However, the knowledge of the ecological role, physiology, function, and genomics of endemic hydrocarbon consumers is still limited. Here, we investigated the dynamics of phenanthrene-consuming communities derived from the Antarctic soil and found that Variovorax, Rhodocyclaceae, and Hydrogenophaga were differentiated in all the phenanthrene-consuming subcultures. We isolated a pure culture of the key hydrocarbon consumer Variovorax sp. strain N23. Moreover, the result of the polyphasic approach suggested that strain N23 represents a novel species of the genus Variovorax. In addition, the genomic characteristics of this strain revealed incomplete degradation pathways for diverse hydrocarbons. Overall, this study reveals the relatively weak hydrocarbon-degrading potential of the indigenous bacteria and suggests the need for more careful protection of the Antarctic ecosystem.

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“…This method of clean-up has been efficient in removing 60% of hydrocarbon contaminants from polluted soils near Carlini Station, King George Island, using bacteria such as Pseudomonas sp. [9]. To date, bioremediation approaches examined in Antarctica have focused on the use of bacteria, fungi and yeasts [10,11].…”

Section: Introductionmentioning

confidence: 99%

The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel

Zamree,

Puasa,

Lim

et al. 2023

Plants

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Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degraders. Therefore, this study focused on the utilisation of an Antarctic microalga in the bioremediation of diesel. The studied microalgal strain was originally obtained from a freshwater ecosystem in Paradise Bay, western Antarctic Peninsula. When analysed in systems with and without aeration, this microalgal strain achieved a higher growth rate under aeration. To maintain the growth of this microalga optimally, a conventional one-factor-at a-time (OFAT) analysis was also conducted. Based on the optimized parameters, algal growth and diesel degradation performance was highest at pH 7.5 with 0.5 mg/L NaCl concentration and 0.5 g/L of NaNO3 as a nitrogen source. This currently unidentified microalga flourished in the presence of diesel, with maximum algal cell numbers on day 7 of incubation in the presence of 1% v/v diesel. Chlorophyll a, b and carotenoid contents of the culture were greatest on day 9 of incubation. The diesel degradation achieved was 64.5% of the original concentration after 9 days. Gas chromatography analysis showed the complete mineralisation of C7–C13 hydrocarbon chains. Fourier transform infrared spectroscopy analysis confirmed that strain WCY_AQ5_3 fully degraded the hydrocarbon with bioabsorption of the products. Morphological and molecular analyses suggested that this spherical, single-celled green microalga was a member of the genus Micractinium. The data obtained confirm that this microalga is a suitable candidate for further research into the degradation of diesel in Antarctica.

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Identification of key bacterial players during successful full-scale soil field bioremediation in Antarctica

Cited by 9 publications

References 64 publications

Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures

Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures

Characterization and Hydrocarbon Degradation Potential of Variovorax sp. Strain N23 Isolated from the Antarctic Soil

The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel

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