Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells

Fonseca, C.H. d'Àvila; Cunha‐Neto, Edécio; Kalil, Jorge; Jesus, AR de; Corrêa-Oliveira, Rodrigo; Em, Carvalho; Oliveira, S. C.

doi:10.1590/s0074-02762004000900011

Cited by 23 publications

(11 citation statements)

References 18 publications

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“…T cell epitopes of paramyosin are recognized by resistant individuals (69,70) IrV5 (fragment of myosin heavy chain) 32% − 75% protection was largely antibody dependent (71) 28% protection in baboons was antibody dependent (49) Specific humoral and cellular responses have been demonstrated in humans (70,72) TPI (triose phosphate isomerase) 30-60% protection is both antibody and CMI dependent (73) No data available…”

Section: Rodentsmentioning

confidence: 99%

“…Consistently > 50% protection has been obtained (74,75) No data available T cell epitopes of Sm14 are recognized by resistant individuals (69,70) Sm23 (an integral membrane protein) 40 -50% protection is associated with antibody and CMI responses (76) No data available Specific humoral and cellular responses have been demonstrated in humans (72)…”

Section: Rodentsmentioning

confidence: 99%

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Resistance to re‐infection after exposure to normal and attenuated schistosome parasites in the baboon model

Kariuki

Farah

2005

Parasite Immunology

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The baboon model of schistosomiasis has been used extensively to study parasite biology, immune responses and pathological manifestations after natural and experimental infections. The body of knowledge accumulated so far has placed this animal model at the pinnacle in the continuing search for new interventions and might hold the key to the development of new anti-schistosome vaccines. In this review paper, we highlight previous and recent studies that have elevated the baboon to be the model of choice for schistosomiasis research. In particular, the long-term studies of re-infection after chemotherapy as well as the interaction between vaccination, chemotherapy and infection are highlighted.

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Section: Rodentsmentioning

confidence: 99%

Section: Rodentsmentioning

confidence: 99%

Resistance to re‐infection after exposure to normal and attenuated schistosome parasites in the baboon model

Kariuki

Farah

2005

Parasite Immunology

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“…These results confirm Para (210-226) promiscuity, but what makes this peptide interesting in composing an anti-schistosome vaccine is its ability to induce cellular responses in individuals resistant to S. mansoni reinfection compared to infected patients. In a preliminary study performed by our group using far fewer subjects, we also demonstrated the ability of Para (210-226) peptide to induce proliferative responses in individuals resistant to reinfection [24]. A recent study performed with mice infected with S. mansoni has shown that chronic infection is related to IL-10 production, which can be responsible for the Th2 type of immune response observed after egg deposition [25].…”

mentioning

confidence: 61%

“…This assay showed that Para (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29), Para (210-226) and Para (355-371) were able to bind to 100% (8/8) of the HLA-DR molecules used in this assay,…”

Section: Paramyosin Peptide Binding To Mhc Class II Moleculesmentioning

confidence: 91%

Identification of paramyosin T cell epitopes associated with human resistance toSchistosoma mansonireinfection

Fonseca

Cunha-Neto

Goldberg

et al. 2005

Clinical and Experimental Immunology

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SummaryParamyosin, a Schistosoma mansoni myoprotein associated with human resistance to infection and reinfection, is a candidate antigen to compose a subunit vaccine against schistosomiasis. In this study, 11 paramyosin peptides selected by TEPITOPE algorithm as promiscuous epitopes were produced synthetically and tested in proliferation and in vitro human leucocyte antigen (HLA)-DR binding assays. A differential proliferative response was observed in individuals resistant to reinfection compared to individuals susceptible to reinfection in response to Para (210-226) peptide stimulation. In addition, this peptide was able to bind to all HLA-DR molecules tested in HLA-DR binding assays, confirming its promiscuity. Para (6-22) and Para (355-371) were also shown to be promiscuous peptides, because they were able to bind to the six and eight most prevalent HLA-DR alleles used in HLA-DR binding assays, respectively, and were also recognized by T cells of the individuals studied. These results suggest that these paramyosin peptides are promising antigens to compose an anti-schistosomiasis vaccine.

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“…3). The tegument associated antigens expressed on newly transformed and developing schistosomules and involved in important host-parasite interactions are important candidates for vaccine development 23 .…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of the Gene Encoding SJCHGC 03921 Protein of the Lung Stage of Schistosoma Mansoni (7-Days Schistosomula)

Mahgoub

2010

BESPS

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The parasitic helminth Schistosoma mansoni (S. mansoni) is a major public health concern in many developing countries. Over 200 million people have, and another 600 million are at risk of contracting schistosomiasis which is one of the major neglected tropical diseases. For this dangerous disease the development of longlasting immunity through vaccination may be the real solution to control the spread of the disease. The molecules on the surface or associated with the tegument of S. mansoni are a major focus as potential vaccine candidates. In the present study, all surface and internal proteins of the lung stage of the parasite were screened to increase the chances for the discovery of a unique protein of the parasite to be targeted by the immune system of the host. Pooled sera were collected from S. mansoni chronically infected patients, then, purified over a column made of soluble extract of the lung stage (7-days schistosomula) of S. mansoni. The eluted antibodies were used to immunoscreen λgt11 cDNA library of 7-days schistosomula. A number of cDNA clones were identified after three rounds of immunoscreening and plaques purification. The phage DNAs of the isolated clones were amplified by polymerase chain reaction (PCR) using λgt11 forward and reverse primers, then, cloned in PCR TM II plasmid vector. The isolated clone 4-65 was fully sequenced and was found encoding the gene of SJCHGC 03921 protein of 7-days schistosomula of S. mansoni. Also, the 0.9 kb cDNA clone was found to have a single open reading frame (ORF) encoding 269 amino acids, which exhibited 94% homology with the gene of SJCHGC 03921 protein of Schistosoma japonicum.

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Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells

Cited by 23 publications

References 18 publications

Resistance to re‐infection after exposure to normal and attenuated schistosome parasites in the baboon model

Resistance to re‐infection after exposure to normal and attenuated schistosome parasites in the baboon model

Identification of paramyosin T cell epitopes associated with human resistance toSchistosoma mansonireinfection

Molecular Characterization of the Gene Encoding SJCHGC 03921 Protein of the Lung Stage of Schistosoma Mansoni (7-Days Schistosomula)

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