Identification of <i>Entamoeba polecki</i> with Unique 18S <scp>rRNA</scp> Gene Sequences from Celebes Crested Macaques and Pigs in Tangkoko Nature Reserve, North Sulawesi, Indonesia

Tuda, Josef S. B.; Feng, Min; Imada, Mihoko; Kobayashi, Seiki; Cheng, Xunjia; Tachibana, Hiroshi

doi:10.1111/jeu.12304

Cited by 15 publications

(13 citation statements)

References 21 publications

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“…Until very recently, ST4 had been detected only in human hosts. However, Tuda et al (21), found ST4 in 75 Celebes crested macaques (Macaca nigra) in Tangkoko, North Sulawesi, Indonesia. In that study, ST4 appeared to be a practically consistent finding in these animals, being detected in 97.4% of these monkeys.…”

Section: Discussionmentioning

confidence: 99%

“…In that study, ST4 appeared to be a practically consistent finding in these animals, being detected in 97.4% of these monkeys. Regarding the study by Tuda et al (21), it remains unclear whether all cases of ST4 were confirmed by sequencing or whether the subtype-specific PCR designed for the study (see above) was used diagnostically. Nevertheless, at least one sample from a crested macaque was identified molecularly as ST4 (Table S1).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, at least one sample from a crested macaque was identified molecularly as ST4 (Table S1). In that study (21), a species-specific PCR was developed for the detection of ST4. However, the forward primer Epo-IMN-F (5=-AGG ATT TGT TTA ATA CAG-3=), which was apparently based on the sequence with GenBank accession no.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of a PCR Method for Detection of Entamoeba polecki, with an Overview of Its Molecular Epidemiology

et al. 2018

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is a parasite of human and nonhuman primates, other mammals, and birds. Due to overlapping morphological features, cysts of may be confused with those of other species commonly found in human fecal samples, including immature cysts of Although the presence of in human -positive stool samples may be rare, its prevalence is likely underestimated due to such confusion. Here, we give examples of diagnostic approaches applied so far and summarize data on the molecular epidemiology of, including host specificity and phylogeography. Moreover, we evaluate a novel diagnostic conventional PCR developed for the screening of fecal samples for The assay was highly sensitive and specific when used on genomic DNA extracted directly from stool and Swedish wastewater samples. The PCR enabled the identification of all four subtypes (ST1 to ST4) of by PCR product sequencing. Most (23/28) subtypedpositive samples detected in patients in Sweden between 2002 and 2015 reflected colonization by ST4 and were seen in travelers/foreigners. Two and three human cases of ST2 and ST3, respectively, were also detected. Subtypes 1, 2, and 3 were detected in 3/21 wastewater samples, suggesting local endemicity of these subtypes; interestingly, ST4 was not detected in wastewater. In conclusion, the current PCR assay enables simple and cost-effective screening of fecal and wastewater samples for Human cases of appear to involve primarily ST4, while detected in wastewater may be primarily of animal origin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of a PCR Method for Detection of Entamoeba polecki, with an Overview of Its Molecular Epidemiology

et al. 2018

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“…For a long time, E. polecki ST4 was only known from humans. Recently, however, ST4 was found in wild Celebes crested macaques ( Macaca nigra ) [20]. Although E. polecki is considered less pathogenic to humans or animals in the case of solo infection, coinfections with other pathogens, such as Lawsonia intracellularis , may increase the severity of the disease [3].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Identification of Three Entamoeba Species in Pigs in Southeastern China

Cao

et al. 2019

BioMed Research International

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Parasitic Entamoeba spp. can infect many classes of vertebrates including humans and pigs. Entamoeba suis and zoonotic Entamoeba polecki have been identified in pigs, and swine are implicated as potential reservoirs for Entamoeba histolytica. However, the prevalence of Entamoeba spp. in pigs in southeastern China has not been reported. In this study, 668 fecal samples collected from 6 different regions in Fujian Province, southeastern China, were analyzed to identify three Entamoeba species by nested PCR and sequencing analysis. The overall prevalence of Entamoeba spp. was 55.4% (370/668; 95% CI 51.6% to 59.2%), and the infection rate of E. polecki ST1 was the highest (302/668; 45.2%, 95% CI 41.4% to 49.0%), followed by E. polecki ST3 (228/668; 34.1%, 95% CI 30.5% to 37.7%) and E. suis (87/668; 13.0%, 95% CI 10.5% to 15.6%). E. histolytica was not detected in any samples. Moreover, the coinfection rate of E. polecki ST1 and ST3 was 25.1% (168/668; 95% CI 21.9% to 28.4%), the coinfection rate of E. polecki ST1 and E. suis was 3.7% (25/668; 95% CI 2.3% to 5.2%), the coinfection rate of E. polecki ST3 and E. suis was 0.3% (2/668), and the coinfection rate of E. polecki ST1, E. polecki ST3, and E. suis was 4.0% (27/668; 95% CI 2.5% to 5.5%). A representative sequence (MK347346) was identical to the sequence of E. suis (DQ286372). Two subtype-specific sequences (MK357717 and MK347347) were almost identical to the sequences of E. polecki ST1 (FR686383) and ST3 (AJ566411), respectively. This is the first study to survey the occurrence and to conduct molecular identification of three Entamoeba species in southeastern China. This is the first report regarding mixed infections with E. suis, E. polecki ST1, and E. polecki ST3 in China. More research studies are needed to better understand the transmission and zoonotic potential of Entamoeba spp.

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“…E. suis is thought to induce hemorrhagic colitis by invading the lamina propria (Matsubayashi et al 2014). E. polecki ST1 and ST3 are associated with proliferative enteritis caused by Li (Matsubayashi et al 2015a(Matsubayashi et al , 2015b(Matsubayashi et al , 2016, although the ST2 and ST4 subtypes, which have been detected in humans and nonhuman primates (Jirků-Pomajbíková et al 2016, Tuda et al 2016, have not been reported in pigs. E. histolytica infection has only been observed in miniature pigs in an experimental setting, but never in farmed animals (He et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Retrospective and Histopathological Studies of <i>Entamoeba</i> spp. and Other Pathogens Associated with Diarrhea and Wasting in Pigs in Aichi Prefecture, Japan

Komatsu¹,

Matsubayashi

Murakoshi

et al. 2019

JARQ

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known to be involved in postweaning wasting (Ellis et al. 1998, Järveots et al. 2016). Salmonellosis, particularly that associated with Salmonella Typhimurium and Salmonella Choleraesuis, can occur concurrently with PMWS (Ha et al. 2005). The enteric protozoan parasites Entamoeba spp., especially E. histolytica, E. polecki (Matsubayashi et al. 2015a, 2015b, 2016), and E. suis (Matsubayashi et al. 2014), were recently identified as the causative agent of diarrhea in pigs. E. polecki can be classified into four subtypes (ST1-ST4) by polymerase chain reaction (PCR) and nucleotide sequencing of the small-subunit ribosomal

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Identification of Entamoeba polecki with Unique 18S rRNA Gene Sequences from Celebes Crested Macaques and Pigs in Tangkoko Nature Reserve, North Sulawesi, Indonesia

Cited by 15 publications

References 21 publications

Evaluation of a PCR Method for Detection of Entamoeba polecki, with an Overview of Its Molecular Epidemiology

Evaluation of a PCR Method for Detection of Entamoeba polecki, with an Overview of Its Molecular Epidemiology

Prevalence and Genetic Identification of Three Entamoeba Species in Pigs in Southeastern China

Retrospective and Histopathological Studies of <i>Entamoeba</i> spp. and Other Pathogens Associated with Diarrhea and Wasting in Pigs in Aichi Prefecture, Japan

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