Identification of
            <i>Bartonella</i>
            Species Directly in Clinical Specimens by PCR-Restriction Fragment Length Polymorphism Analysis of a 16S rRNA Gene Fragment

Matar, Ghassan M.; Koehler, Jane E.; Malcolm, G B; Lambert-Fair, Mary Ann; Tappero, Jordan W.; Hunter, Suzan B.; Swaminathan, Bala

doi:10.1128/jcm.37.12.4045-4047.1999

Cited by 45 publications

(9 citation statements)

References 21 publications

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“…PCR involves amplification of Bartonella species genes (16S rRNA gene, citrate synthase gene (gltA), and htrA gene) directly from tissue or aspirate. It is specific but with a variable sensitivity (40–70%) with high risk of negative results if sample collection occurs too early (<6 weeks) [ 47 , 57 – 61 ]. PCR testing of the lymph node was not performed in our case since it is an invasive procedure and requires sedation in pediatric patients.…”

Section: Discussionmentioning

confidence: 99%

Osteomyelitis in Cat-Scratch Disease: A Never-Ending Dilemma—A Case Report and Literature Review

Donà

Fovino

Mozzo

et al. 2018

Case Reports in Pediatrics

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Background We performed a review of published case studies of osteomyelitis associated with cat-scratch disease to consolidate existing information on clinical presentation, diagnostic tools, therapy, and outcome, as well as presenting a case of disseminated cat-scratch disease in a 12-year-old female with skull osteomyelitis and spleen involvement. Methods A search for articles indexed in PubMed, Embase, and Google Scholar was performed with the search terms “Bartonella,” “bone,” “osteomyelitis,” “osteolytic,” and “cat-scratch disease” limited to the immunocompetent pediatric population and articles in English. Results 51 cases were identified. The average age was 7.8 years with equal sex distribution. Fever (84.3%), often with a prolonged course (64.7%), and osteoarticular pain (88.2%) were the most common clinical findings. Lymphadenopathy was present in 64.7% of patients. Vertebral body was mainly involved (51.9%). MRI (50%) and bone scintigraphy (48.1%) were favored to confirm osteomyelitis, while serology was the preferred microbiological diagnostic. Various antibiotics were prescribed in combined or sequential regimens, with median duration of therapy of 23 days. About 12.5% of patients did not receive any treatment. Most patients had excellent prognosis; in particular, all patients not receiving any therapy showed complete recovery and no recurrence of symptoms. Conclusions Bartonella henselae should be considered in differential diagnosis of localized lymphadentitis. Osteoarticular pain or limitation during cat-scratch disease in children should always be investigated for bone spreading. Owing to good prognosis, invasive procedures to obtain the bone material should be avoided. Serology is the gold standard diagnostic tool and MRI is the best radiographic technique to define bone and surrounding tissue involvement. Treatment represents a never-ending dilemma: surgical intervention or use of antibiotics is still controversial, and more studies are needed to define the best antimicrobial regimen.

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Section: Discussionmentioning

confidence: 99%

Osteomyelitis in Cat-Scratch Disease: A Never-Ending Dilemma—A Case Report and Literature Review

Donà

Fovino

Mozzo

et al. 2018

Case Reports in Pediatrics

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“…The implication of the Bartonella species in a variety of human diseases and the difficulty of its isolation from clinical specimens highlight the need for the development of better detection and identification methods. 43 In fact, both B. quintana and B. henselae were found to cause endocarditis and previously identified by PCR-RFLP. 32 , 43 This assay might open new promising opportunities for the early diagnosis of IE and accurate differentiation between species.…”

Section: Resultsmentioning

confidence: 92%

“… 43 In fact, both B. quintana and B. henselae were found to cause endocarditis and previously identified by PCR-RFLP. 32 , 43 This assay might open new promising opportunities for the early diagnosis of IE and accurate differentiation between species. 43 …”

Section: Resultsmentioning

confidence: 92%

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A broad-range PCR technique for the diagnosis of infective endocarditis

Boujelben

Gdoura

Hammami

2018

Brazilian Journal of Microbiology

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Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and evaluated in terms of its efficiency in the diagnosis of endocarditis using 19 heart valves from patients undergoing cardiovascular surgeries at the Habib Bourguiba Hospital of Sfax, Tunisia, on the grounds of suspected IE. The results demonstrated the efficiency of this technique particularly in cases involving a limited number of bacteria since it helped to increase detection sensitivity. The technique proved to be efficient, particularly, in the bacteriological diagnosis of IE in contexts involving negative results from conventional culture methods and other contexts involving bacterial species that were not amenable to identification by phenotypic investigations. Indeed, the sequencing of the partial 16S ribosomal RNA gene revealed the presence of Bartonella henselae, Enterobacter sp., and Streptococcus pyogenes in three heart valves with the negative culture. It should be noted that the results obtained from the polymerase chain reaction-sequencing identification applied to the heart valve and the strain isolated from the same tissue were not consistent with the ones found by the conventional microbiological methods in the case of IE caused by Gemella morbillorum. In fact, the results from the molecular identification revealed the presence of Lactobacillus jensenii. Overall, the results have revealed that the proposed method is sensitive, reliable and might open promising opportunities for the early diagnosis of IE.

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“…A nested PCR using the cell division gene ftsZ to differentiate among B. bacilliformis, B. quintana, and B. henselae was described by Kelly et al (258). Additional approaches include several PCR restriction fragment length polymorphism (RFLP) methods, including use of RNA polymerase beta subunit (rpoB) gene analysis (259), the citrate synthase (gltA) gene (260,261), the ftsZ gene (262), and the 16S rRNA gene (263). Molecular analyses based on only one-step PCRs have also been described.…”

Section: Molecular Techniquesmentioning

confidence: 99%

Carrion's Disease: the Sound of Silence

2018

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Carrion's disease (CD) is a neglected biphasic vector-borne illness related to . It is found in the Andean valleys and is transmitted mainly by members of the genus but also by blood transfusions and from mother to child. The acute phase, Oroya fever, presents severe anemia and fever. The lethality is high in the absence of adequate treatment, despite the organism being susceptible to most antibiotics. Partial immunity is developed after infection by , resulting in high numbers of asymptomatic carriers. Following infection there is the chronic phase, Peruvian warts, involving abnormal proliferation of the endothelial cells. Despite potentially being eradicable, CD has been expanded due to human migration and geographical expansion of the vector. Moreover, studies have demonstrated the risk of the development of antimicrobial resistance. These findings, together with the description of new species producing CD-like infections, the presence of undescribed potential vectors in new areas, the lack of adequate diagnostic tools and knowledge of the immunology and bacterial pathogenesis of CD, and poor international visibility, have led to the risk of increasing the potential expansion of resistant strains which will challenge current treatment schemes as well as the possible appearance of CD in areas where it is not endemic.

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Identification of Bartonella Species Directly in Clinical Specimens by PCR-Restriction Fragment Length Polymorphism Analysis of a 16S rRNA Gene Fragment

Cited by 45 publications

References 21 publications

Osteomyelitis in Cat-Scratch Disease: A Never-Ending Dilemma—A Case Report and Literature Review

Osteomyelitis in Cat-Scratch Disease: A Never-Ending Dilemma—A Case Report and Literature Review

A broad-range PCR technique for the diagnosis of infective endocarditis

Carrion's Disease: the Sound of Silence

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