Identification of hydrocarbon-oxidizing Dietzia bacteria from petroleum reservoirs based on phenotypic properties and analysis of the 16S rRNA and gyrB genes

Abstract

The taxonomic position of hydrocarbon oxidizing bacterial strains 263 and 32d isolated from for mation water of the Daqing petroleum reservoir (PRC) was determined by polyphasic taxonomy techniques, including analysis of the 16S rRNA and the gyrB genes. The major chemotaxonomic characteristics of both strains, including the IV type cell wall, composition of cell wall fatty acids, mycolic acids, and menaquinones, agreed with those typical of Dietzia strains. The DNA G+C content of strains 263 and 32d were 67.8 and 67.6 mol %, respectively. Phylogenetic analysis of the 16S rRNA gene of strain 32d revealed 99.7% similarity to the gene of D. maris, making it possible to identify strain 32d as belonging to this species. The 16S rRNA gene sequence of strain 263 exhibited 99.7 and 99.9% similarity to those of D. natronolimnaea and D. cerci diphylli YIM65002 T , respectively. Analysis of the gyrB genes of the subterranean isolates and of a number of Dietzia type strains confirmed classification of strain 32d as a D. maris strain and strain 263 as a D. natrono limnaea strain. A conclusion was made concerning higher resolving power of phylogenetic analysis of the gyrB gene compared to the 16S rRNA gene analysis in the case of determination of the species position of Dietzia isolates. rRNA and gyrB genes, primers for amplification and sequencing of the gyrB genes in bacteria of the genus Dietzia

Selective biostimulation of cold‐ and salt‐tolerant hydrocarbon‐degrading Dietzia maris in petroleum‐contaminated sub‐Arctic soils with high salinity

“…(A) and (B)) deviates considerably from the halotolerance of Dietzia maris strains 7824 A and 4‐3 previously reported by Borzenkov et al Dietzia maris strains 7824 A and 4‐3 grow optimally in the absence of NaCl and at relatively lower salinities, below 2% NaCl . Halotolerant oil‐degrading Dietzia maris strain DSM 43672 T grows well between 0 and 8% NaCl . In the present study, NWWC4 was able to grow at 12.5% NaCl, albeit at a slower rate.…”

“…32 Halotolerant oil-degrading Dietzia maris strain DSM 43672 T grows well between 0 and 8% NaCl. 33 In the present study, NWWC4 was able to grow at 12.5% NaCl, albeit at a slower rate. Halophilic oil-degrading Dietzia sp.…”

Low-Abundance Dietzia Inhabiting a Water-Flooding Oil Reservoir and the Application Potential for Oil Recovery

“…Recently, several Dietzia strains have been isolated from oil reservoirs [25–27]. Despite the low abundance, Dietzia sp.…”

“…Our previous investigation indicated that low-abundance Dietzia was generally detected in oil reservoirs, including 22 reservoirs with distinct different formation temperatures and salinity, across North, Northeast, and Northwest of China [24]. In addition, several Dietzia strains have been isolated from oil reservoirs and were found able to utilize hydrocarbons [25–27]. However, despite that, our knowledge about the oil recovery potential of these low-abundance microbial populations is poor.…”

A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons

“…Dietzia sp. has been described for alkane and aromatic hydrocarbon degradation activities ( Nazina et al, 2015 ). However, described as a bacterial species characterizing many environmental matrices, suggesting interesting adaptation capabilities, Dietzia sp., is still poorly described, and a new fundamental ecological role will be clarified soon.…”