Identification of Human Remains by <scp>DNA</scp> Analysis of the Gastrointestinal Contents of Fly Larvae

Chávez-Briones, María de Lourdes; Hernández-Cortés, B S Raquel; Díaz-Torres, B S Porfirio; Niderhauser‐García, Alberto; Áncer‐Rodríguez, Jesús; Jaramillo‐Rangel, Gilberto; Ortega‐Martínez, Marta

doi:10.1111/j.1556-4029.2012.02279.x

Cited by 21 publications

(11 citation statements)

References 16 publications

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“…The likelihood of paternity was 99.685%. The results were verified by analyzing DNA isolated from bone (requiring multiple samples, as stressed by the au thors), which made it possible to obtain a higher probability level [44].…”

Section: Autorzy Deklarują Brak Konfliktu Interesówmentioning

confidence: 88%

“…Pierw szy opis przypadku zastosowania takiego podejścia (a zarazem wykorzystania entomologii sądowej w Meksyku) opublikowano dopiero w 2013 r. [44]. W opisanej sprawie wykorzystano zawartość prze vicina flies.…”

Section: Wykorzystanie śLadów Entomologicznych Jako źRódła Ludzkiego unclassified

“…The first case report of such an approach (and, at the same time, the application of forensic entomology in Mexico) was only published in 2013 [44]. The case involved an analysis of the alimen tary canal content of Calliphoridae and Sarcophagi-Archiwum Medycyny Sądowej i Kryminologii Archives of Forensic Medicine and Criminology wodu pokarmowego larw Calliphoridae i Sarcophagidae ujawnionych na spalonych zwłokach kobiety do ustalenia pokrewieństwa z domniemanym ojcem ofiary.…”

Section: Use Of Entomological Evidence As a Source Of Human Dna In Prmentioning

confidence: 99%

“…Prawdopodobieństwo ojcostwa wyniosło 99,685%. Wyniki te były weryfikowane analizą DNA wyizolowanego z kości (wymagającą wielu prób, jak zaznaczyli autorzy), co pozwoliło na uzyskanie wyż szej wartości prawdopodobieństwa [44].…”

Section: Use Of Entomological Evidence As a Source Of Human Dna In Prunclassified

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Insects feeding on cadavers as an alternative source of human genetic material

Skowronek¹,

Tomsia²,

Droździok³

et al. 2014

amsik

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StreszczenieW niektórych sprawach kryminalnych wykorzystanie klasycznych źródeł ludzkiego materiału genetycznego jest utrud nione lub nawet niemożliwe. Rozwiązaniem może być wykorzystanie owadów, zwłaszcza larw muchówek, żerujących na zwłokach. Aktualny przegląd opisów przypadków oraz badań eksperymentalnych dostępnych w bazach biomedycz nych wykazał, że owady mogą stanowić cenne źródło ludzkiego kwasu dezoksyrybonukleinowego (DNA), zarówno mitochondrialnego, jak i genomowego, umożliwiającego efektywną analizę, odpowiednio sekwencji regionów hiper zmiennych (HVR) i profili krótkich powtórzeń tandemowych (STR). Optymalnym źródłem ludzkiego DNA jest wole (część jelita) aktywnych larw muchówek III stadium. Puparia i wydaliny owadów również mogą być wykorzystane w praktyce genetycznosądowej zamiast zawartości przewodu pokarmowego.Słowa kluczowe: entomologia sądowa, genetyka sądowa, larwy muchówek, identyfikacja osobnicza. AbstractIn some criminal cases, the use of classical sources of human genetic material is difficult or even impossible. One so lution may be the use of insects, especially blowfly larvae which feed on corpses. A recent review of case reports and experimental studies available in biomedical databases has shown that insects can be a valuable source of human mito chondrial and genomic deoxyribonucleic acid (DNA), allowing for an effective analysis of hypervariable region (HVR) sequences and short tandem repeat (STR) profiles, respectively. The optimal source of human DNA is the crop (a part of the gut) of active thirdinstar blowfly larvae. Pupae and insect faeces can be also used in forensic genetic practice instead of the contents of the alimentary tract.Key words: forensic entomology, forensic genetics, blowfly larvae, personal identification. WprowadzenieEntomologia sądowa stopniowo zyskuje coraz większe uznanie wśród przedstawicieli organów ści

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Section: Autorzy Deklarują Brak Konfliktu Interesówmentioning

confidence: 88%

Section: Wykorzystanie śLadów Entomologicznych Jako źRódła Ludzkiego unclassified

Section: Use Of Entomological Evidence As a Source Of Human Dna In Prmentioning

confidence: 99%

Section: Use Of Entomological Evidence As a Source Of Human Dna In Prunclassified

See 2 more Smart Citations

Insects feeding on cadavers as an alternative source of human genetic material

Skowronek¹,

Tomsia²,

Droździok³

et al. 2014

amsik

View full text Add to dashboard Cite

show abstract

“…STR polymorphisms have high discrimination power, require a small quantity of sample for testing, and can be used to test old samples. Because of these advantages, STR polymorphisms have been used as the major genetic markers for DNA identification in the field of forensic medicine . Nowadays, commercially available kits allow testing of not only autosomal STR polymorphisms but also sex chromosome STR polymorphisms, and these tools play important roles in DNA identification in forensic medicine .…”

Section: Amplification Conditions For Human Nails By Direct Pcr Usingmentioning

confidence: 99%

Detection of short tandem repeat polymorphisms from human nails using direct polymerase chain reaction method

Tie

Uchigasaki

2014

Electrophoresis

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Human nail is an important forensic material for parental testing and individual identification in large-scale disasters. Detection of STR polymorphism from hard tissues generally requires DNA purification, which is technically complicated and time consuming. In the present study, we attempted to detect STR polymorphisms from untreated human nail samples by direct PCR amplification method using the primer mixture supplied with the GenePrint® SilverSTR® III System or the AmpFℓSTR® Identifiler® PCR Amplification Kit, and Tks Gflex DNA polymerase known to be effective for amplification from crude samples. A nail fragment measuring approximately 1.5 mm in breadth and 0.5 mm in length was placed directly into a PCR tube, and various PCR conditions were tested. The PCR products were analyzed by denaturing acrylamide gel electrophoresis or CE. Multiple STR polymorphisms were detected successfully. This method that detects STR polymorphisms not only from fresh human fingernails, but also from old nail fragments stored at room temperature for up to 10 years is expected to become a novel DNA analytical method in forensic medicine and genetic studies.

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DNA Profiling from Burnt Remains

Zapico

Stone-Gordon

2023

Burnt Human Remains

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Identification of Human Remains by DNA Analysis of the Gastrointestinal Contents of Fly Larvae

Cited by 21 publications

References 16 publications

Insects feeding on cadavers as an alternative source of human genetic material

Insects feeding on cadavers as an alternative source of human genetic material

Detection of short tandem repeat polymorphisms from human nails using direct polymerase chain reaction method

DNA Profiling from Burnt Remains

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