Identification of Hippophae species (Shaji) through DNA barcodes

Liu, Yue; Sun, Wei; Liu, Chuan; Zhang, Yaqin; Chen, Yilong; Song, Ming; Fan, Gang; Liu, Xia; Li, Xiang; Zhang, Yi

doi:10.1186/s13020-015-0062-9

Cited by 27 publications

(13 citation statements)

References 27 publications

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“…Currently, the Nrf2/ARE signaling pathway appears to be the most important endogenous antioxidant stress pathway [65,66]. Furthermore, Nrf2 is pivotal in the host defense mechanism, and numerous key phase II enzymes, such as NQO1 and HO-1 [67], are controlled by Nrf2 transcription factor under ROS conditions [4].…”

Section: Effects On Cell Antioxidant Responsesmentioning

confidence: 99%

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Advanced Research on the Antioxidant Activity and Mechanism of Polyphenols from Hippophae Species—A Review

Gong

et al. 2020

Molecules

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Oxidation is a normal consequence of metabolism in biological organisms. The result is the formation of detrimental reactive oxygen species (ROS) and reactive nitrogen species (RNS). A large number of studies have shown that polyphenolic compounds have good antioxidant properties. Hippophae species plants have high polyphenolic content and are widely used in food, medicinal, or the cosmetic field. The main polyphenols in Hippophae species are flavonoids, phenolic acids and tannins, which have multiple effects. However, there is a limited number of studies on polyphenols in Hippophae species plants. This review systematically summarizes the polyphenols compounds and antioxidant activity of Hippophae species plants, and it is noteworthy that the main mechanisms of the polyphenols of Hippophae with antioxidant activity have been summarized as follows: regulating enzyme activity, affect the antioxidant reaction of cells, and others. This review provides useful information for the further study and application of Hippophae species polyphenols and their antioxidant activity.

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Section: Effects On Cell Antioxidant Responsesmentioning

confidence: 99%

“…Hippophae is a member of the Elaeagnaceae family, in which 7 species and 11 subspecies have been identified worldwide [4]. Hippophae is widely used in various fields, such as the food, medicine and cosmetic industries.…”

Section: Introductionmentioning

confidence: 99%

Advanced Research on the Antioxidant Activity and Mechanism of Polyphenols from Hippophae Species—A Review

Gong

et al. 2020

Molecules

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“…Plant metabolomics has recently attracted considerable attention because the method focuses on the holistic characteristics, and has been developed as an important approach for the modern research of (medicinal) plants. LC/GC-MS and 1 H NMR are the most commonly used analytical strategies in metabolomics 22 – 26 . Compared with the two chromatographic techniques, 1 H NMR based metabolomics is considered as the most promising analytical tool because the method allows simultaneously detection of primary and secondary metabolites in a single run, and produces a non-biased abundant metabolic profile.…”

Section: Introductionmentioning

confidence: 99%

Metabolic discrimination of sea buckthorn from different Hippophaë species by 1H NMR based metabolomics

Liu

Fan

Zhang

et al. 2017

Sci Rep

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Sea buckthorn (Hippophaë; Elaeagnaceae) berries are widely consumed in traditional folk medicines, nutraceuticals, and as a source of food. The growing demand of sea buckthorn berries and morphological similarity of Hippophaë species leads to confusions, which might cause misidentification of plants used in natural products. Detailed information and comparison of the complete set of metabolites of different Hippophaë species are critical for their objective identification and quality control. Herein, the variation among seven species and seven subspecies of Hippophaë was studied using proton nuclear magnetic resonance (1H NMR) metabolomics combined with multivariate data analysis, and the important metabolites were quantified by quantitative 1H NMR (qNMR) method. The results showed that different Hippophaë species can be clearly discriminated and the important interspecific discriminators, including organic acids, L-quebrachitol, and carbohydrates were identified. Statistical differences were found among most of the Hippophaë species and subspecies at the content levels of the aforementioned interspecific discriminators via qNMR and one-way analysis of variance (ANOVA) test. These findings demonstrated that 1H NMR-based metabolomics is an applicable and effective approach for simultaneous metabolic profiling, species differentiation and quality assessment.

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“…The internal transcribed spacer 2 (ITS2) region has been proposed as a candidate DNA barcode for medicinal plants Han et al, 2016;Xiang et al, 2015;Yao et al, 2010). At present, the DNA barcoding technique has been successfully used to identify traditional Chinese medicines from adulterants and closely related species (Hu et al, 2015;Liu et al, 2015;Pang et al, 2013;Pang et al, 2011), such as Corni Fructus , Aristolochiaceous herbs (Wu et al, 2015), Ganoderma species (Liao et al, 2015), and Rhodiola species (Xin et al, 2015). Therefore, this study used the ITS2 region to distinguish Fritillariae bulbus from other species and adulterants.…”

Section: Accepted Manuscriptmentioning

confidence: 97%

Identification of Fritillariae bulbus from adulterants using ITS2 regions

et al. 2016

Plant Gene

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Distinguishing the five types of Fritillariae bulbus (Fritillariae cirrhosae bulbus, Fritillariae ussuriensis bulbus, Fritillariae pallidiflorae bulbus, Fritillariae thunbergii bulbus and Fritillariae hupehensis bulbus)is difficult because of their similar morphological, physical, and chemical characteristics. These species, which are included in the Chinese Pharmacopoeia, exhibit different therapeutic effects. Therefore, we used internal transcribed spacer 2 (ITS2) regions as DNA barcode to distinguish the five types of Fritillariae bulbus from each other and their adulterants. A total of 151 samples belonging to five types of Fritillariae bulbus (a total of 11 species of Fritillaria) and two adulterant species were collected. The phylogeny was constructed using the neighbor joining (NJ), maximum parsimony (MP), and maximum likelihood (ML) methods. Genomic DNAs were successfully extracted from all bulbus samples and obtaining high-quality ITS2 sequences via polymerase chain reaction optimization. The length of the ITS2 sequence of 12 Fritillaria species ranged from 235 bp to 241 bp, and the average GC content was over 68%. Adulterants Bolbostemma paniculatum and F. maximowiczii could be A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPTdistinguished from all Fritillariae bulbus species by using the distance and tree methods. The ML tree showed better topology than the NJ and MP trees. Members of each type of Fritillariae bulbus formed one clade, which was strongly supported with bootstrap values, except for Fritillariae thunbergii bulbus and Fritillariae hupehensis bulbus in the ML tree. Therefore, DNA barcoding technology based on ITS2 regions can be used to distinguish the five types of Fritillariae bulbus from each other and their adulterants except for Fritillariae thunbergii bulbus and Fritillariae hupehensis bulbus.

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Identification of Hippophae species (Shaji) through DNA barcodes

Cited by 27 publications

References 27 publications

Advanced Research on the Antioxidant Activity and Mechanism of Polyphenols from Hippophae Species—A Review

Advanced Research on the Antioxidant Activity and Mechanism of Polyphenols from Hippophae Species—A Review

Metabolic discrimination of sea buckthorn from different Hippophaë species by 1H NMR based metabolomics

Identification of Fritillariae bulbus from adulterants using ITS2 regions

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