Biochemical Pharmacology volume 77, issue 2, P269-276 2009 DOI: 10.1016/j.bcp.2008.09.027 View full text
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Inga I. Kramarenko, Marlene A. Bunni, Thomas A. Morinelli, John R. Raymond, Maria N. Garnovskaya

Abstract: AbstractThe Human Embryonic Kidney (HEK) 293 cell line is widely used in cell biology research. Although HEK293 cells have been meticulously studied, our knowledge about endogenous G protein-coupled receptors (GPCR) in these cells is incomplete. While studying the effects of bradykinin (BK), a potent growth factor for renal cells, we unexpectedly discovered that BK activates extracellular signalregulated protein kinase 1 and 2 (ERK) in HEK293 cells. Thus, we hypothesized that HEK293 cells possess endogenous B…

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