Identification of four novel subgenotypes (C13–C16) and two inter-genotypic recombinants (C12/G and C13/B3) of hepatitis B virus in Papua Province, Indonesia

Mulyanto, Mulyanto; Pancawardani, Pingki; Depamede, Sulaiman Ngongu; Wahyono, Arif; Jirintai, Suljid; Nagashima, Shigeo; Takahashi, Masaharu; Nishizawa, Tsutomu; Okamoto, Hiroaki

doi:10.1016/j.virusres.2011.09.002

Cited by 51 publications

(46 citation statements)

References 52 publications

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“…HBV subgroups have been identified within different HBV genotypes when an intra-genotype difference of À4z and º 8z was applied to the complete nucleotide sequence. This approach resulted in subgroups A1-A7 of HBV genotype A, B1-B9 of HBV genotype B, C1-C16 of HBV genotype C, D1-D8 of HBV genotype D, and F1-F4 of HBV genotype F, with distinct geographical distributions (18)(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Correlation between Hepatitis B Virus Genotypes and Clinical Outcomes

Shi¹

2012

Jpn J Infect Dis

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Section: Introductionmentioning

confidence: 99%

Correlation between Hepatitis B Virus Genotypes and Clinical Outcomes

Shi¹

2012

Jpn J Infect Dis

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“…This means that efforts to prevent the spread of HBV in Indonesia will be more effective if we can provide the components to develop diagnostic kits derives from the local germ plasma. This should become a serious concern for Indonesia with a population of more than 200 million people with the HBV carrier rates are 4-20.3% (Khan et al, 2004;Mulyanto et al, 2008). CONCLUSION This study shows that antibody generated using the local horses can be utilized to develop test kit against infectious diseases such as Hepatitis B with feasible results.…”

Section: Resultsmentioning

confidence: 86%

“…Recently it has been reported that in Indonesia some new HBV genotypes have been elucidated (Lusida et al, 2008, Mulyanto et al, 2009, 2010, 2012. This means that efforts to prevent the spread of HBV in Indonesia will be more effective if we can provide the components to develop diagnostic kits derives from the local germ plasma.…”

Section: Resultsmentioning

confidence: 99%

“…Around the world, over 350 million people are infected chronically with HBV, some of them will develop severe liver disease including cirrhosis and hepatocellular carcinoma (Mulyanto et al, 2011(Mulyanto et al, , 2012. The highest prevalence of HBV infection is generally found in Asia and Africa (Lee, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…In Indonesia the prevalence of HBV varies among the islands. It has been reported that HBV carrier rates among apparently healthy populations range from 4 to 20.3% (Khan et al, 2004;Mulyanto et al, 2008). Several efforts to prevent the spread of HBV have been conducted by the Indonesian government, such as through a national vaccination program of children and do the screening of blood donors carried out by the Indonesian Red Cross.…”

Section: Introductionmentioning

confidence: 99%

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Utilization of Polyclonal Antibodies Produced in Local Horses (Equus Caballus) as a Resource for Development of Elisa Conjugate to Detect Hepatitis B Virus (Hbv) Surface Antigens

Depamede

Sriasih

Yulianti

2012

J. Indonesian Trop. Anim. Agric.

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ABSTRAKTujuan dari penelitian ini adalah memanfaatkan antibodi poliklonal yang diproduksi dari kuda (Equus caballus) lokal Indonesia untuk membuat konjugat kit ELISA pendeteksi antigen permukaan virus hepatitis B (VHB; HBsAg). Antibodi poliklonal yang digunakan adalah dari kuda lokal yang diimunisasi berulang menggunakan HBsAg isolat Indonesia. Antibodi kemudian dikonjugasikan dengan enzim horseradish peroxidase dengan metode yang dimodifikasi dari Nakane dan Kawaoi. Uji aktivitas konjugat dilakukan dengan prinsip uji ELISA yang dikerjakan oleh peneliti dan juga oleh laboratorium independen. Sebagai pembanding, digunakan konjugat ELISA HBsAg komersial. Hasil penelitian ini menunjukkan bahwa antibodi yang diproduksi dari kuda lokal dapat digunakan untuk membuat konjugat dengan hasil yang sebanding dengan kit ELISA HBsAg komersial Kata kunci: Kuda lokal, ELISA, antibodi poliklonal, hepatitis B, HBsAg ABSTRACTThe aim of this study was to utilize the antibody produced using Indonesia local horses (Equus caballus) to make the conjugate of ELISA kit for detection of hepatitis B virus (HBV) surface antigen (HBsAg). The polyclonal antibodies were isolated and purified from local horses immunized repeatedly using isolated and purified HBsAg from Indonesia. The antibodies were conjugated with horseradish peroxidase by a modified method of Nakane and Kawaoi. The conjugate activities were performed using the principle of ELISA test conducted by the researchers as well as by independent laboratory. Commercial conjugate for HBsAg ELISA was used as a comparison study. The results of this study indicated that the antibody produced from local horses can be used to make conjugates that were comparable to commercial HBsAg ELISA kit.

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Hepatitis B virus candidate subgenotype I1 varies in distribution throughout Guangxi, China and may have originated in Long An county, Guangxi

Li¹,

Hué²,

Harrison

et al. 2013

Journal of Medical Virology

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Sequencing of the complete hepatitis B virus (HBV) genomes from Vietnam, China and Laos led to the identification of a complex recombinant, referred to initially as an aberrant genotype and later proposed to be a new genotype, I. However, epidemiological data regarding this new genotype are lacking. A cross-sectional study was carried out to investigate the epidemiology of HBV candidate genotype I in Guangxi, China using stratified, random cluster sampling. Four thousand five hundred thirteen subjects were recruited from five counties within Guangxi. Three genotypes, B, C, and I, were identified with a prevalence of 32.6% (114/350), 64% (224/350), and 3.4% (12/350), respectively. All the genotype I isolates belong to candidate subgenotype I1 and were found in Bing Yang (15.3%, 9/59) and Na Po (5.0%, 3/60) counties only. The prevalence of this subgenotype is significantly higher in males (5.1%, 10/195) than in females (1.3%, 2/155; X(2) = 3.959, P < 0.05) but does not differ significantly with age. It was found in the Han (4.5%, 9/201) and Zhuang (3.1%, 3/97) ethnic populations only. There is no significant difference from other genotypes in the prevalence of HBV serological markers. Phylogeographic analysis revealed that genotype I1 likely arose in Long An county, then spread later to Bing Yang, Na Po counties and elsewhere in southeast Asia. In conclusion, the distribution of candidate genotype I within Guangxi is not even and it is highly endemic in some counties. Its prevalence is associated with gender and ethnicity. Subgenotype I1 likely originated in Long An county.

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Identification of four novel subgenotypes (C13–C16) and two inter-genotypic recombinants (C12/G and C13/B3) of hepatitis B virus in Papua Province, Indonesia

Cited by 51 publications

References 52 publications

Correlation between Hepatitis B Virus Genotypes and Clinical Outcomes

Correlation between Hepatitis B Virus Genotypes and Clinical Outcomes

Utilization of Polyclonal Antibodies Produced in Local Horses (Equus Caballus) as a Resource for Development of Elisa Conjugate to Detect Hepatitis B Virus (Hbv) Surface Antigens

Hepatitis B virus candidate subgenotype I1 varies in distribution throughout Guangxi, China and may have originated in Long An county, Guangxi

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