Identification of fluoroquinolone-resistant extended-spectrum  -lactamase (CTX-M-8)-producing Escherichia coli ST224, ST2179 and ST2308 in buffalo (Bubalus bubalis)

Aizawa, Juliana; Neuwirt, Natalie; Barbato, Leandro; Neves, Patrícia; Leigue, Lucianne; Padilha, João; Castro, Antonio Fernando Pestana de; Gregory, Lílian; Lincopán, Nilton

doi:10.1093/jac/dku218

Cited by 33 publications

(18 citation statements)

References 10 publications

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“…Notably, both ST10 and ST224 previously demonstrated their capacity for nosocomial infections and the spread of these sequence types between horses and potentially, to their human handlers [14,18,19]. In addition to being recovered from horses [14], ST224 was isolated from humans [11], swine [42], bovines [47], birds [44], and in this study, from a donkey. ST167 E. coli was isolated from humans, cattle, swine, wild birds [34,46,48], turkey meat [43], and horses [49].…”

Section: Discussionmentioning

confidence: 58%

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

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Simple Summary: The spread and development of extended spectrum beta-lactamase (ESBL)-mediated antimicrobial resistance is a significant concern in healthcare with impacts to animal and public health alike. While the occurrence of the ESBL phenotype in Escherichia coli has been investigated in depth by numerous studies, there is still a lack of information regarding ESBL-producing bacterial isolates from clinical specimens of equine origin. In this study, we investigated the incidence of ESBL-producing E. coli in hospitalized horses. Overall, 207 E. coli isolates were analyzed for their antimicrobial susceptibility and 13 ESBL-producing E. coli isolates were genotypically characterized. Seven out of the 13 E. coli isolates were found to harbor the resistance genes bla CTX-M-1 or bla SHV-1 and a novel beta-lactamase TEM gene variant, bla TEM-233 was discovered. Furthermore, despite being phenotypically susceptible to tested carbapenems, 1 out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 . The latter is an alarming finding because the presence of carbapenemase resistance genes in equine pathogens is extremely rare. In conclusion, equines can be reservoirs for ESBL-producing Enterobacteriaceae, and further investigation into this species group is necessary to understand their impact in the spread and development of antibiotic resistance genes.Abstract: Escherichia coli isolates were recovered from clinical specimens of equine patients admitted to the Texas A&M Veterinary Medical Teaching Hospital over a five-year period. Ceftiofur resistance was used as a marker for potential extended-spectrum beta-lactamase (ESBL)-activity, and of the 48 ceftiofur-resistant E. coli isolates, 27.08% (n = 13) were phenotypically ESBL-positive. Conventional PCR analysis followed by the large-scale bla Finder multiplex PCR detected the ESBL genes, CTX-M-1 and SHV, in seven out of the 13 isolates. Moreover, beta-lactamase genes of TEM-1-type, BER-type (AmpC), and OXA-type were also identified. Sequencing of these genes resulted in identification of a novel TEM-1-type gene, called bla TEM-233 , and a study is currently underway to determine if this gene confers the ESBL phenotype. Furthermore, this report is the first to have found E. coli ST1308 in horses. This subtype, which has been reported in other herbivores, harbored the SHV-type ESBL gene. Finally, one out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 despite the lack of phenotypically proven resistance to imipenem. With the identification of novel ESBL gene variant and the demonstrated expansion of E. coli sequence types in equine patients, this study underscores the need for more investigation of equines as reservoirs for ESBL-producing pathogens. ]. The wide application of beta-lactam antibiotics has been considered as a driving factor in the development and spread of extended-spectrum beta-lactamase (ESBL)-conferred resistance in Gram-negative bacterial pathogens such as Escherichia coli, Klebsiella spp., and Sa...

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Section: Discussionmentioning

confidence: 58%

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

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“…The majority of sequence types found among the FQ and ESC resistant isolates in the current study (ST354, ST10, S648 and ST 224) have been previously described globally among humans as well as animals Aizawa et al, 2014;Dahms et al, 2015;Day et al, 2016;Dissanayake et al, 2014;Kim et al, 2011;Maluta et al, 2014;Manges and Johnson, 2012;Sola-Gines et al, 2015;Vignaroli et al, 2012). Nonetheless, four of the FQ resistant FEC isolates belonged to the same sequence type (ST354), but were cultured from different geographical regions indicating that they did not originate from one shed.…”

Section: Investigating the Prevalence Of Carriage Of Avian Pathogenicsupporting

confidence: 51%

“…The current study reported a variation in the ST among the clinical FQ resistant isolates (keeping in mind that there was only two clinical FQ resistant isolates) with one assigned as ST224 while the other was ST10. Both STs have previously been identified in horses, dogs, buffalos and human pathogenic and non-pathogenic E. coli Aizawa et al, 2014;Dissanayake et al, 2014;Maluta et al, 2014;Manges and Johnson, 2012;Pires-dos-Santos et al, 2013;Vignaroli et al, 2012;Zhao et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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“…These enzymes are encoded by bla CTX-M -type gene associated with mobile genetic elements, including insertion sequences ISEcp1 type and ISCR1, most carried on transferable plasmids (Zhao and Hu, 2013). In Brazil, although the production of the CTX-M enzymes has become the most prevalent mechanism of acquired resistance to broad-spectrum cephalosporins in gram-negative bacteria from clinical samples, poultry, food-producing animals, hospital wastewater and urban rivers (Aizawa et al, 2014;Carvalho-Assef et al, 2014;Ferreira et al, 2014;Peirano et al, 2011;Silva et al, 2013;Tollentino et al, 2011), there are no data available regarding the detection of CTX-M-producing bacteria in chicken meat intended for sale on the Brazilian market, which is curious, since the presence of CTX-M-2-and CTX-M-8-producing E. coli in raw chicken and broiler meat imported into European countries from South America countries, including Brazil, has been previously reported (Dhanji et al, 2010;Egervärn et al, 2014;Warren et al, 2008). So, the aim of this study was to investigate the presence of ESBL-producing bacteria and the genetic background of genes encoding ESBLs in retail chicken meat from markets, in southeastern Brazil.…”

Section: Introductionmentioning

confidence: 99%

Detection of blaCTX-M-type genes in complex class 1 integrons carried by Enterobacteriaceae isolated from retail chicken meat in Brazil

Casella

Rodríguez

Takahashi

et al. 2015

International Journal of Food Microbiology

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Identification of fluoroquinolone-resistant extended-spectrum -lactamase (CTX-M-8)-producing Escherichia coli ST224, ST2179 and ST2308 in buffalo (Bubalus bubalis)

Cited by 33 publications

References 10 publications

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Detection of blaCTX-M-type genes in complex class 1 integrons carried by Enterobacteriaceae isolated from retail chicken meat in Brazil

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