Identification of fatty acids in canine seminal plasma

Díaz, Rommy; Inostroza, Karla; Risopatrón, Jennie; Sánchez, Raúl; Sepúlveda, Néstor

doi:10.1111/and.12070

Cited by 7 publications

(13 citation statements)

References 11 publications

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“…In fact, in our experiment, a higher concentration of fatty acids, such as stearic and pentadecenoic fatty acids, was observed in the epididymal cauda fluid, where sperm reaches its highest degree of maturation. Such fatty acids were observed previously in ejaculated semen in dogs [ 20 , 42 ]. In addition, we observed increase in saturated fatty acids between the epididymal corpus and cauda, but not for DHA concentration.…”

Section: Discussionsupporting

confidence: 77%

“…Despite the importance of sperm lipid profile, the exact composition of the fatty acids that structure sperm plasma membrane has not yet been explored for epididymal samples in dogs, only in ejaculated semen [ 19 ] and seminal plasma [ 20 ]. Moreover, the exact mechanism that structures sperm plasma membrane should be investigated in dogs.…”

Section: Introductionmentioning

confidence: 99%

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Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

Angrimani

Nichi

Losano

et al. 2017

J Animal Sci Biotechnol

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BackgroundDuring sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy, computer-assisted motility analysis, sperm plasma membrane permeability and the fatty acid analysis (lipids were extracted, transmethylated and analyzed by chromatography).ResultsCaput and corpus sperm showed lower values for the motility variables evaluated and plasmatic membrane integrity, indicating different levels of the fatty acids organization. Saturated, monounsaturated and polyunsaturated fatty acids were in higher concentrations in the spermatozoa from epididymis cauda. Highlighting the presence of caprylic, stearic and docosahexaenoic acids.ConclusionsThese findings demonstrate the influence of the fatty acid profile during sperm maturation, assigning physical and chemical changes in sperm cells, essential for fertilization.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

Angrimani

Nichi

Losano

et al. 2017

J Animal Sci Biotechnol

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show abstract

“…Considering that, in dogs, PUFA are not detected or are found in low proportions in seminal plasma ( Díaz et al , 2014 ), in this work, we used spermatic fraction to measure FA profile as a reliable indicator of the sperm PUFA profile.…”

Section: Resultsmentioning

confidence: 99%

Association between dietary polyunsaturated fatty acids and their concentration in blood plasma, red blood cell and semen of dogs

Pellegrino¹,

Corrada²,

Picco³

et al. 2023

Open Vet J

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Background: In dogs, dietary omega 3 polyunsaturated fatty acids (n-3 PUFA) affect the fatty acid (FA) profile of blood plasma, erythrocyte membrane (EM) and semen, but their correlation has not yet been investigated. Aim: In this study, we evaluated the association between dietary PUFA and their profile in blood plasma, EM and semen of dogs, with the possibility to predict the semen profile using the values of the three first. Methods: Twelve male dogs received the same standard commercial diet for four weeks. The FA profile was analyzed by gas chromatography in paired diet, blood (plasma and EM determinations) and semen samples. Data were analyzed with SAS Proc Corr version 9.4. Pearson´s correlation coefficient (significant if p < 0.05) was used to assess the association of dietary FA profiles with those in blood plasma, EM and semen. Results: There was a positive correlation between dietary eicosapentaenoic acid (EPA) and blood plasma (r = 0.97), EM (r = 0.94) and semen (r = 0.92) EPA, and between dietary docosahexaenoic acid (DHA) and arachidonic acid (ARA) and semen DHA (r = 0.93) and ARA (r = 0.92), respectively. There was negative correlation between dihomo-gamma-linolenic acid (DGLA) in the diet and EM DGLA (r = -0.94). Conclusion: The dietary EPA is correlated with blood plasma, EM and semen EPA concentrations, and dietary DHA and ARA are correlated with semen DHA and ARA concentrations in dogs. These findings suggest that dietary EPA, DHA and ARA concentrations could be useful predictive markers for such concentrations in the semen of dogs.

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“…In boar spermatozoa, the most abundant SFAs were C16:0 (18%) and C18:0 (16%), and the most abundant UFAs were DPA (15%) and DHA (16%) (Waterhouse et al ., ). Nevertheless, in dog seminal plasma were observed a low proportion of PUFA and the predominant fatty acids were C16:0 (30.4%), C18:0 (23.4%) and C18:1n9 (9.0%) (Díaz et al ., ). Moreover, in human spermatozoa, the main SFAs were C16:0 and C18:0 that both together represented 90% of the total SFA.…”

Section: Discussionmentioning

confidence: 97%

Determination of fatty acid profile in ram spermatozoa and seminal plasma

Díaz

Torres²,

Bravo

et al. 2015

Andrologia

Self Cite

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Fatty acids are important in male reproductive function because they are associated with membrane fluidity, acrosome reaction, sperm motility and viability, but limited information exists about the fatty acid profile of ram semen. Our aim was to determine the fatty acid composition in ram spermatozoa and seminal plasma. Sixty ejaculates were obtained from three ram (20 ejaculates/ram) using artificial vagina. Ram spermatozoa (RS) and seminal plasma (SP) were separated using centrifugation, and the fatty acids were analysed by gas chromatography. Total lipids obtained in ram spermatozoa were 1.8% and 1.6% in seminal plasma. Saturated fatty acid (SFA) was proportionally major in SP (66.6%) that RS (49.9%). The highest proportions of SFA corresponded to C4:0 (RS = 16.3% and SP = 28.8%) and C16:0 (RS = 16.3% and PS = 20%). The most important unsaturated fatty acid (UFA) was docosahexaenoic acid (DHA), 44.9% in RS and 31.5% in SP. The profile of fatty acid and their proportions showed differences between spermatozoa and seminal plasma.

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Identification of fatty acids in canine seminal plasma

Cited by 7 publications

References 11 publications

Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

Association between dietary polyunsaturated fatty acids and their concentration in blood plasma, red blood cell and semen of dogs

Determination of fatty acid profile in ram spermatozoa and seminal plasma

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