Identification of dysregulated microRNA expression and their potential role in the antiproliferative effect of the essential oils from four different Lippia species against the CT26.WT colon tumor cell line

Gomide, Mayna da Silveira; Lemos, Fernanda O.; Reis, Danièle; José, Gustavo; Lopes, Míriam Teresa Paz; Machado, M. A.; Alves, Tânia Maria de Almeida; Coelho, Cíntia Marques

doi:10.1016/j.bjp.2016.04.003

Cited by 9 publications

(4 citation statements)

References 40 publications

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“…These studies have helped us to understand the genetic organization and evolution of Lippia species, and to characterize the germplasm bank for biological, pharmacological, and agricultural research. Their pharmacological properties have been shown to be associated with their secondary metabolites, specifically their essential oils (Gomide et al, 2016;Pascual et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…The genus Lippia includes 88 species, of which 68 are endemic to Brazil (Salimena and Múlgura, 2015). Gomide et al (2016) evaluated the anti-proliferative effect of essential oils from four species of Lippia in CT26.WT colon tumor cells by measuring the cell cycle phase distribution and miRNA expression. CT26.WT cells showed cell cycle arrest in G2/M phase after specific treatment.…”

Section: Introductionmentioning

confidence: 99%

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MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

José

Campos

Viccini

et al. 2019

Rev. Agric. Neotrop.

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Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

José

Campos

Viccini

et al. 2019

Rev. Agric. Neotrop.

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“…Os miRNAs são reguladores mestres dos genes do ciclo celular em diferentes tipos de câncer. Os resultados dos estudos mostram que 27,36% dos miRNAs estavam desregulados, deste modo, os autores acreditam que o mecanismo de ação, para os efeitos encontrados na indução da parada do ciclo celular, envolve a expressão diferencial de miRNAs oncogênicos chave (GOMIDE et al, 2016).…”

Section: Neuroativa E Anti-inflamatóriaunclassified

Avanços E Novas Descobertas Sobre O Uso De Erva Cidreira (Lippia Alba) Para Inovação Terapêutica Na Última Década (2010-2020)/Advances and New Discoveries on the Use of Ervacidreira (Lippia Alba) for Therapeutic Innovation in the Last Decade (2010-2020)

Lima

Lins

2020

BJD

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“…Numerous studies have been conducted to unveil the significant outcomes of isoprenoid-based manipulations of the mevalonate pathway and HMGCR in cancer cells. γ-Tocotrienol at 0–30 μM downregulated HMGCR, membrane H-, K-, and N-Ras, and Raf-1, p-AKT, and p-ERK in HL-60 cells (Chen et al, 2015), adding to a long list of in vitro and in vivo studies showing isoprenoid-mediated suppression of the growth of cancer cells including those of blood (Shoff et al, 1991; Melnykovych et al, 1992; Mo and Elson, 1999; Lee et al, 2015), breast (Iqbal et al, 2004; Pierpaoli et al, 2010, 2013; Gomide et al, 2013; Ding et al, 2017), cervix (Yazlovitskaya and Melnykovych, 1995; Xu et al, 2017; Potocnjak et al, 2018), colon (Mo and Elson, 1999; Gomide et al, 2013, 2016), liver (Wada et al, 2005; Crespo et al, 2013; Scolastici et al, 2014; Rodenak-Kladniew et al, 2018), lung (Mo and Elson, 1999; Wada et al, 2005; Gomide et al, 2013; Galle et al, 2014), mouth (Liang et al, 2013; Madankumar et al, 2013), pancreas (Hussein and Mo, 2009; Fernandes et al, 2013), prostate (Mo and Elson, 2004; Sundin et al, 2012; Fernandes et al, 2013; Jones et al, 2013; Yeganehjoo et al, 2017), skin (Mo et al, 2000; McAnally et al, 2007; Chang et al, 2009; Chaudhary et al, 2013), and stomach (Dong et al, 2013; Liu et al, 2013). Concomitantly, isoprenoids induce cell cycle arrest and apoptosis in the tumor cells (Mo and Elson, 1999, 2004; Dong et al, 2013; Jones et al, 2013; Yeganehjoo et al, 2017; Rodenak-Kladniew et al, 2018; Sailo et al, 2018).…”

Section: Isoprenoids and Their Mechanisms Of Actionmentioning

confidence: 99%

The Potential of Isoprenoids in Adjuvant Cancer Therapy to Reduce Adverse Effects of Statins

Jeter

Bachmann

et al. 2019

Front. Pharmacol.

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The mevalonate pathway provides sterols for membrane structure and nonsterol intermediates for the post-translational modification and membrane anchorage of growth-related proteins, including the Ras, Rac, and Rho GTPase family. Mevalonate-derived products are also essential for the Hedgehog pathway, steroid hormone signaling, and the nuclear localization of Yes-associated protein and transcriptional co-activator with PDZ-binding motif, all of which playing roles in tumorigenesis and cancer stem cell function. The phosphatidylinositol-4,5-bisphosphate 3-kinase-AKT-mammalian target of rapamycin complex 1 pathway, p53 with gain-of-function mutation, and oncoprotein MYC upregulate the mevalonate pathway, whereas adenosine monophosphate-activated protein kinase and tumor suppressor protein RB are the downregulators. The rate-limiting enzyme, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), is under a multivalent regulation. Sterol regulatory element binding protein 2 mediates the sterol-controlled transcriptional downregulation of HMGCR. UbiA prenyltransferase domain-containing protein-1 regulates the ubiquitination and proteasome-mediated degradation of HMGCR, which is accelerated by 24, 25-dihydrolanosterol and the diterpene geranylgeraniol. Statins, competitive inhibitors of HMGCR, deplete cells of mevalonate-derived intermediates and consequently inhibit cell proliferation and induce apoptosis. Clinical application of statins is marred by dose-limiting toxicities and mixed outcomes on cancer risk, survival and mortality, partially resulting from the statin-mediated compensatory upregulation of HMGCR and indiscriminate inhibition of HMGCR in normal and tumor cells. Tumor HMGCR is resistant to the sterol-mediated transcriptional control; consequently, HMGCR is upregulated in cancers derived from adrenal gland, blood and lymph, brain, breast, colon, connective tissue, embryo, esophagus, liver, lung, ovary, pancreas, prostate, skin, and stomach. Nevertheless, tumor HMGCR remains sensitive to isoprenoid-mediated degradation. Isoprenoids including monoterpenes (carvacrol, L-carvone, geraniol, perillyl alcohol), sesquiterpenes (cacalol, farnesol, β-ionone), diterpene (geranylgeranyl acetone), “mixed” isoprenoids (tocotrienols), and their derivatives suppress the growth of tumor cells with little impact on non-malignant cells. In cancer cells derived from breast, colon, liver, mesothelium, prostate, pancreas, and skin, statins and isoprenoids, including tocotrienols, geraniol, limonene, β-ionone and perillyl alcohol, synergistically suppress cell proliferation and associated signaling pathways. A blend of dietary lovastatin and δ-tocotrienol, each at no-effect doses, suppress the growth of implanted murine B16 melanomas in C57BL6 mice. Isoprenoids have potential as adjuvant agents to reduce the toxicities of statins in cancer prevention or therapy.

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Identification of dysregulated microRNA expression and their potential role in the antiproliferative effect of the essential oils from four different Lippia species against the CT26.WT colon tumor cell line

Cited by 9 publications

References 40 publications

MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

Avanços E Novas Descobertas Sobre O Uso De Erva Cidreira (Lippia Alba) Para Inovação Terapêutica Na Última Década (2010-2020)/Advances and New Discoveries on the Use of Ervacidreira (Lippia Alba) for Therapeutic Innovation in the Last Decade (2010-2020)

The Potential of Isoprenoids in Adjuvant Cancer Therapy to Reduce Adverse Effects of Statins

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