Identification of Cucumber Mosaic Virus Subgroup I Isolates from Banana Plants Affected by Infectious Chlorosis Disease Using RT-PCR

Singh, Zora; Jones, R. A. C.; Jones, Molly Morgan

doi:10.1094/pd-79-0713

Cited by 44 publications

(31 citation statements)

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“…Previously, RT-PCR based detection of CMV subgroup-I and -II was achieved based on MspI restriction pattern [18,26] and immunocapture RT-PCR [35] using CP gene. In the present study, molecular detection by RT-PCR was developed to detect CMV subgroups in Indian population of CMV.…”

Section: Discussionmentioning

confidence: 99%

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

et al. 2011

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Cucumber mosaic virus (CMV) is one of the most important viral pathogen infecting several plant species in India. Five isolates of CMV obtained from cucumber, muskmelon, tobacco and tomato from distinct geographical locations in India were analysed based on host-reactions and genome sequence. The majority of the isolates were very similar and only two isolates, Tfr-In and Tss-In showed distinct symptoms in tomato and high sequence diversity (77.8%) in coat protein (CP) gene. Tfr-In was isolated from tomato fruit showing grey patches in Aurangabad and Tss-In from tomato plant showing shoe-string symptoms in New Delhi. The RNA-3 genomes of Tfr-In (2,214 nt; JF279606), shared only 70.3% nucleotide sequence identity with Tss-In (2,178 nt; JF279605. The complete RNA-3 genome of Tss-In and Tfr-In were compared with that of 65 CMV isolates reported from various plants of the world, which formed four distinct subclades-IA, -IB, -IC and -II. The Tfr-In isolate clustered with the CMV subgroup-IB and Tss-In with the subgroup-II. The comparison of the RNA-3 sequence of both the isolates revealed maximum heterogeneity in the intergenic region (IR). Reverse transcriptase polymerase chain reaction (RT-PCR) based detection of CMV subgroup-I and -II was developed designing primers from flanking IR region. The specificity of the RT-PCR detection was confirmed using Tfr-In and Tss-In representing subgroup-I and -II and validated with field samples of tomato, cucurbits and chilli. This is the first report of complete RNA-3 of subgroup-IB CMV causing grey patches in tomato fruit and subgroup-II CMV causing shoe-string symptoms in tomato in India. The present and previous studies together showed that tomato in India was affected by multiple strains of CMV.

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Section: Discussionmentioning

confidence: 99%

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

et al. 2011

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“…Indeed, the RT-PCR method has proven to be more sensitive than ELISA and dot-blot hybridization (Hu et al, 1995). Also cucumber mosaic virus (CMV) can be effectively detected with different sets of degenerate primers (Choi, et al 1999;Hu et al, 1995;Singh et al, 1995). The set of primers used in this study amplified the CP ORF and flanking regions of CMV isolates from ornamental crops successfully.…”

Section: Discussionmentioning

confidence: 99%

Alstroemeria-Infecting Cucumber Mosaic Virus Isolates Contain Additional Sequences in the Rna 3 Segment

Chen¹,

Prins²,

Derks³

et al. 2002

Acta Hortic.

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The coat protein (CP) genes and flanking regions of three alstroemeriainfecting cucumber mosaic virus isolates (CMV-ALS), denoted ALS-LBO, ALS-IPO, and ALS-NAK, were cloned and their nucleotide sequence determined and compared at both nucleic acid and deduced protein level with the published sequences of CMV RNA 3. The sequences of these isolates showed more than 95% nucleotide and peptide sequence homology to each other and to other members of subgroup II CMV. Strikingly, an additional sequence of 218 nt was found in the central region of the 3'-non-translated region (NTR) of RNA 3 of two out of three isolates. The additional sequence appeared to have arisen from RNAs 1 or 2. A subgroup-specific DIG-labeled probe has been developed from this additional sequence and applied to detect subgroup II CMV strains in dot blot hybridization and to differentiate the Alstroemeria isolates containing additional sequences in Northern blot hybridization.

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“…Cucumovirus, Family: Bromoviridae) is considered the most plant virus of agricultural importance in the world (Jones & McKirdy 1990, Palukaitis et al, 1992, Singh et al, 1995. CMV has the widest host rang of any known viruses, since it infect more than 1200 species belong to over than 100 families of dicotyledonous and monocotyledonous (Edwardson & Christie 1991).…”

Section: Cucumber Mosaic Cucumovirus (Cmv Genusmentioning

confidence: 99%

Amaryllis and Shrimp plant are secondary hosts of Cucumber mosaic cucumovirus (CMV) in Iraq

Adhab¹,

Al-Ani²

2011

ABJNA

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Identification of Cucumber Mosaic Virus Subgroup I Isolates from Banana Plants Affected by Infectious Chlorosis Disease Using RT-PCR

Cited by 44 publications

References 0 publications

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

Alstroemeria-Infecting Cucumber Mosaic Virus Isolates Contain Additional Sequences in the Rna 3 Segment

Amaryllis and Shrimp plant are secondary hosts of Cucumber mosaic cucumovirus (CMV) in Iraq

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