Identification of Clostridium chauvoei in clinical samples cultures from blackleg cases by means of PCR

Miyashiro, Simone; Nassar, Alessandra Figueiredo de Castro; Souza, Maria Conceição Aparecida Macedo; Carvalho, Jessica Blenda Fernandes; Adegas, J.E.B.

doi:10.1590/s1517-83822007000300020

Cited by 4 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the clostridial diseases, the myonecrosis (gas gangrene and blackleg) are necrotizing soft tissue infections that affect cattle (ASSIS et al, 2010), sheep and goats (LIMA et al, 2006) leading to significant economic losses due to their high fatality rate (MIYASHIRO et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Most diagnoses are based only on clinical signs and poorly detailed gross lesions, with few reports having laboratory confirmation. The fluorescent antibody test (FAT) and immunohistochemistry (IHC) are the main diagnosis assays, but sometimes the etiological identification, by these techniques, is not enough for final diagnose (LOBATO; ASSIS; SALVARANI, 2007;RIBEIRO et al, 2012). Clostridia are frequent post-mortem invader, and laboratory identification could result in an inadequate diagnosis if there is a lack of tissue samples preservation (ASSIS et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative analysis of lesions caused by histotoxic clostridia in experimentally induced myonecrosis

et al. 2012

View full text Add to dashboard Cite

The comparative anatomical and histopathological lesions caused by different histotoxic clostridia are considered an important step in the diagnosis of epidemiological mionecrosis. In the present study, guinea pigs (Cavia porcellus) were used to reproduce gas gangrene caused by Clostridium septicum, C. chauvoei, C. novyi type A, C. perfringens type A and C. sordellii. The clinical signs, gross and histopathological lesions were compared between inoculated groups and an immunohistochemistry (IHC) was standardized for detection of agents in tissues. On clinical evaluation, animals showed swelling at the injection point, discomfort and limited mobility. The intensity and extent of gross and microscopic lesions varied with the inoculated agent. IHC was able to identify each agents inoculated without cross reactions. All observed results demonstrate that the evaluation of lesions could be usefull in the presumptive etiologic diagnosis. Key words: Blackleg, gas gangrene, myonecrosis, histopathology, immunohistochemistry ResumoO estudo comparativo das lesões anatomo-histopatológicas causadas pelos diferentes clostrídios histotóxicos é considerado uma etapa importante no diagnóstico epidemiológico das mionecroses. Para reproduzir experimentalmente a gangrena gasosa causada por Clostridium septicum, C. chauvoei, C. novyi tipo A, C. perfringens tipo A e C. Sordellii foram utilizados cobaios (Cavia porcellus). Os sinais clínicos, lesões macroscópicas e histopatológicas foram comparadas entre os grupos inoculados e uma imuno-istoquímica (IHQ) foi padronizada para detecção dos agentes nos tecidos das cobaias experimentalmente infectadas. Na avaliação clínica os animais apresentaram aumento de volume no ponto de inoculação, desconforto e dificuldade de locomoção. A intensidade e extensão das lesões macroscópicas e microscópicas variaram com o agente inoculado. Na IHQ foi possível identificar cada um dos agentes inoculados, sem a detecção de reações cruzadas. Todos os resultados observados demonstram que este tipo de avaliação das lesões é de extrema importância para o diagnóstico etiológico conclusivo, possibilitando assim a adoção de medidas preventivas acertivas.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative analysis of lesions caused by histotoxic clostridia in experimentally induced myonecrosis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Polymerase chain reaction (PCR) is a rapid detection strategy for pathogens. When combined with the tissue-impregnated common filter paper samples it eliminates the need for time-consuming procedures associated with DNA extraction (UZAL Espíndola et al et al, 2003;MIYASHIRO et al, 2007;ASSIS et al, 2008). Additionally, filter paper is a low-cost material that is easily reported and enables the shipment of samples without refrigeration (KARIMIAN et al, 2011;SMIT et al, 2014).…”

mentioning

confidence: 99%

“…Advantages of this technique included the easy impregnation of samples by veterinarians or farm owners, low-cost of common filter paper, and the use of normal postal service, without cold storage, for the shipment of samples to the laboratory (SMIT et al, 2014;MATHEUS et al, 2015). As a result, laboratory diagnosis becomes faster, without the need of other techniques to aid in the survival, growth, and enrichment of the bacteria for PCR (MIYASHIRO et al, 2007;ASSIS et al, 2008).…”

mentioning

confidence: 99%

Diagnosis of blackleg from cattle tissue impregnated in common filter paper

Espíndola

Farias

Balzan³

et al. 2021

Cienc. Rural

View full text Add to dashboard Cite

Blackleg, an acute myonecrosis caused by Clostridium chauvoei, is usually underdiagnosed since the rapid transport of adequate samples for laboratory testing is difficult. This study tested a direct polymerase chain reaction (PCR) technique using common filter paper impregnated with cattle tissue samples obtained from animals suspected with blackleg. Twenty-five samples, belonging to eleven animals from Rio Grande do Sul State, Brazil, were analyzed. The direct PCR technique identified eight positive animals corroborating with results from microbiological culture. Skeletal muscle was the most common tissue type used in this study and when the animal was positive the pathogen was always detected in this tissue. Storage time of the impregnated filter paper at room temperature did not prove to be a limiting factor for the quality of the results indicating that this procedure can be carried out in the field and samples be sent in regular mail. Our results suggested that direct PCR of common filter paper impregnated with cattle tissue is a practical and economical alternative for the diagnosis of blackleg.

show abstract

Detection of Clostridium perfringens bacterium in the clinical specimens from blackleg calves: a report of two cases

et al. 2016

View full text Add to dashboard Cite

Identification of Clostridium chauvoei in clinical samples cultures from blackleg cases by means of PCR

Cited by 4 publications

References 12 publications

Comparative analysis of lesions caused by histotoxic clostridia in experimentally induced myonecrosis

Comparative analysis of lesions caused by histotoxic clostridia in experimentally induced myonecrosis

Diagnosis of blackleg from cattle tissue impregnated in common filter paper

Detection of Clostridium perfringens bacterium in the clinical specimens from blackleg calves: a report of two cases

Contact Info

Product

Resources

About