Identification of changes in Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Abstract

In order to understand the molecular basis of salt stress response, a proteomic approach, employing two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), was used to identify proteins affected by salinity in wheat (Triticum durum 'Ofanto'). Identification of proteins, whose levels were altered, was performed by comparing protein patterns of salt-treated and control plants. A set of control plants was grown without NaCl addition under the same conditions as the salt-treated plants. Proteins were extracted from the leaves of untreated and NaCl-treated plants, and resolved using 24-cm immobilized pH gradient strips with a pH 4-7 linear gradient in the first dimension and a 12.5% sodium dodecyl sulphate polyacrylamide gel electrophoresis in the second dimension; the gels were stained with Coomassie and image analysis was performed. Quantitative evaluation, statistical analyses and MALDI-TOF MS characterization of the resolved spots in treated and untreated samples enabled us to identify 38 proteins whose levels were altered in response to salt stress. In particular, ten proteins were downregulated and 28 were upregulated. A possible role of these proteins in response to salinity is discussed. © 2008 Springer-Verlag

Canola Seedling Response to NaCl Stress – a Proteomic Approach

“…1). The same results have been reported for durum wheat (Caruso et al, 2008) and Canola (Bandehagh et al, 2011). The CO2 assimilation is said to be hampered in response to the reduction in the amount of enzymes related to thereof; PRK accelerates the ATP phosphorylation dependent on Ribulose bis-phosphate into Ribulose 1,5-bisphosphate, which is considered an important point in pentose phosphate pathway towards CO2 assimilation.…”

“…Fall in assimilation has been reported for many glycophyte plants subjected to salinity stress (Aghaei, 2008; Pang et al, 2010;Sobhanian et al, 2010;Chatlopadhyay 2011 andBandehagh et al, 2011). However, in some sensitive plants, including durum wheat, an increase in activity of RubisCo is thought to be a response rendered to support the carboxylase activities of RubisCo (Caruso et al, 2008). The F spot is probably related to Phosphoribulokinase (PRK), which has been down-regulated under salinity influence (Fig.…”

“…Proteins with more than one occurrence have previously been 364 detected by other researchers (Caruso et al, 2008;Wang et al 2008;Gao et al, 2011 andBandehagh et al, 2011) the reason for which has been explained as existing protein isoforms, posttranslation modifications, alternative splicing of mRNA and protein breakage. Post-translation modification such as glycosylation and phosphorylation can change molecular weight and isoelectric focusing of the proteins (Caruso et al, 2008).…”

Influence of abiotic stresses on plant proteome and metabolome changes

“…Other enzymes involved in metabolic pathways such as pentose phosphate pathway (phosphoribulokinase) and glycolysis (fructose 1,6-bisphosphate aldolase) showed a decreased abundance, in comparison with control plants. However, enzymes involved in the regulation of carbon metabolism (phosphopyruvate hydratase, triose phosphate isomerase, glucose-6-phosphate dehydrogenase) were upregulated in salt-treated plants (Caruso et al 2008). An increased number of RUBISCO proteolytic fragments was also reported in metal-stressed rice (Hajduch et al 2001).…”

Proteomics Approach for Identifying Salt Stress Related Proteins in Barley Leaves

“…Recently, a number of plant proteomic studies have been published (Mock and Matros, 2008;Hashiguchi et al, 2010). Improvements in proteomic technology regarding protein separation and detection, as well as mass spectrometry based protein identification, have an increasing impact on the study of plant responses to I salinity stress (Caruso et al, 2008). Differential-expression proteomics is based on comparison of composition of different proteomes.…”