Identification of capacitation-associated phosphoproteins in porcine sperm electroporated with ATP-?-32P

Effect of heparin on in vitro capacitation of boar sperm

“…However, the absolute values obtained in each case were different, being higher for CTC. This is in accordance with the fact that CTC shows increased membrane fluidity which occurs well before sperm are able to undergo AR (Tardif et al, 1999).…”

Effect of heparin on in vitro capacitation of boar sperm

“…However, the absolute values obtained in each case were different, being higher for CTC. This is in accordance with the fact that CTC shows increased membrane fluidity which occurs well before sperm are able to undergo AR (Tardif et al, 1999).…”

Cholesterol Efflux Alters Lipid Raft Stability and Distribution During Capacitation of Boar Spermatozoa1

“…Only 10.6% Ϯ 3.3% (n ϭ 7) of control spermatozoa incubated in TALP stained positively with FITC-PNA lectin after exposure to 2 M A23187, whereas after MBCD treatment, 31.9% Ϯ 6.4% (n ϭ 10) of spermatozoa bound the lectin over the acrosomal region. These figures are comparable with those reported by Tardif et al [47], who used BSA to induce capacitation in boar spermatozoa.…”

Capacitation Is Associated with Tyrosine Phosphorylation and Tyrosine Kinase-Like Activity of Pig Sperm Proteins1

“…Others have suggested that capacitation is directly related to sperm tyrosine phosphorylation in various mammalian species [31,32,34]. Our culture conditions induced sperm capacitation in vitro within 3 h in CM (Table 1), which is similar to observations by others [27,[46][47][48][49]. In addition, when the sperm were incubated in CM, considerable headto-head agglutination was evident (data not shown), which is indicative of head plasma membrane modifications due to capacitation [50].…”

Effect of heparin on in vitro capacitation of boar sperm

“…However, the absolute values obtained in each case were different, being higher for CTC. This is in accordance with the fact that CTC shows increased membrane fluidity which occurs well before sperm are able to undergo AR (Tardif et al, 1999).…”

Cholesterol Efflux Alters Lipid Raft Stability and Distribution During Capacitation of Boar Spermatozoa1

“…Only 10.6% Ϯ 3.3% (n ϭ 7) of control spermatozoa incubated in TALP stained positively with FITC-PNA lectin after exposure to 2 M A23187, whereas after MBCD treatment, 31.9% Ϯ 6.4% (n ϭ 10) of spermatozoa bound the lectin over the acrosomal region. These figures are comparable with those reported by Tardif et al [47], who used BSA to induce capacitation in boar spermatozoa.…”

Capacitation Is Associated with Tyrosine Phosphorylation and Tyrosine Kinase-Like Activity of Pig Sperm Proteins1

“…Others have suggested that capacitation is directly related to sperm tyrosine phosphorylation in various mammalian species [31,32,34]. Our culture conditions induced sperm capacitation in vitro within 3 h in CM (Table 1), which is similar to observations by others [27,[46][47][48][49]. In addition, when the sperm were incubated in CM, considerable headto-head agglutination was evident (data not shown), which is indicative of head plasma membrane modifications due to capacitation [50].…”

Effect of heparin on in vitro capacitation of boar sperm

“…However, the absolute values obtained in each case were different, being higher for CTC. This is in accordance with the fact that CTC shows increased membrane fluidity which occurs well before sperm are able to undergo AR (Tardif et al, 1999).…”

Cholesterol Efflux Alters Lipid Raft Stability and Distribution During Capacitation of Boar Spermatozoa1

“…Only 10.6% Ϯ 3.3% (n ϭ 7) of control spermatozoa incubated in TALP stained positively with FITC-PNA lectin after exposure to 2 M A23187, whereas after MBCD treatment, 31.9% Ϯ 6.4% (n ϭ 10) of spermatozoa bound the lectin over the acrosomal region. These figures are comparable with those reported by Tardif et al [47], who used BSA to induce capacitation in boar spermatozoa.…”

Capacitation Is Associated with Tyrosine Phosphorylation and Tyrosine Kinase-Like Activity of Pig Sperm Proteins1

“…Others have suggested that capacitation is directly related to sperm tyrosine phosphorylation in various mammalian species [31,32,34]. Our culture conditions induced sperm capacitation in vitro within 3 h in CM (Table 1), which is similar to observations by others [27,[46][47][48][49]. In addition, when the sperm were incubated in CM, considerable headto-head agglutination was evident (data not shown), which is indicative of head plasma membrane modifications due to capacitation [50].…”