volume 46, issue 2, P593-600 2008
DOI: 10.1128/jcm.01829-07
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Anneke Feberwee, David J. Hampson, Nyree D. Phillips, Tom La, Harold M. J. F. van der Heijden, Gerard J. Wellenberg, R. Marius Dwars, Wil J. M. Landman

Abstract: Cecal samples from laying chickens from 25 farms with a history of decreased egg production, diarrhea, and/or increased feed conversion ratios were examined for anaerobic intestinal spirochetes of the genus Brachyspira. Seventy-three samples positive in an immunofluorescence assay for Brachyspira species were further examined using selective anaerobic culture, followed by phenotypic analysis, species-specific PCRs (for Brachyspira hyodysenteriae, B. intermedia, and B. pilosicoli), and a Brachyspira genus-spec…

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