Anneke Feberwee, David J. Hampson, Nyree D. Phillips, Tom La, Harold M. J. F. van der Heijden, Gerard J. Wellenberg, R. Marius Dwars, Wil J. M. Landman
Cecal samples from laying chickens from 25 farms with a history of decreased egg production, diarrhea, and/or increased feed conversion ratios were examined for anaerobic intestinal spirochetes of the genus Brachyspira. Seventy-three samples positive in an immunofluorescence assay for Brachyspira species were further examined using selective anaerobic culture, followed by phenotypic analysis, species-specific PCRs (for Brachyspira hyodysenteriae, B. intermedia, and B. pilosicoli), and a Brachyspira genus-spec…
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