Identification of bovine CD14

Sopp, P.; Kwong, L.S.; Howard, Christopher

doi:10.1016/0165-2427(96)05583-3

Cited by 44 publications

(16 citation statements)

References 6 publications

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“…Therefore, markers specific for the monocytic lineage have been developed and well characterised for the bovine. Antibodies exist which direct against bovine CD 14 (Berthon and Hopkins 1996;Gupta et al 1996;Sopp et al 1996) and CD 68 (Ackermann et al 1994;Zeng et al 1996;Gutierrez et al 1999). Using the antibody directed against CD 14, we were unable to detect macrophages in embryonic tissues, although these cells were easily recognised with anti-CD 14 in lymph nodes of adult bovines.…”

Section: Lineage Markersmentioning

confidence: 54%

Histochemical in situ identification of bovine embryonic blood cells reveals differences to the adult haematopoietic system and suggests a close relationship between haematopoietic stem cells and primordial germ cells

Kritzenberger

Wrobel

2004

Histochemistry and Cell Biology

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Cryostat sections of bovine embryos of exactly known age (obtained from artificial insemination), ranging from 32 to 60 days post-insemination, were treated with a wide range of antibodies directed against cell surface antigens or lineage-specific factors in order to demonstrate different types of fetal blood cells and their precursors. An antibody specific to bovine c-kit (bk-1) stained not only presumptive haematopoietic stem cells in the dorsal aorta and the embryonic liver, but also a subpopulation of putative primordial germ cells in the gonadal anlage, the latter being further characterised by a positive labelling with the lectins STA, WFA and WGA and a histochemical reaction for alkaline phosphatase. The antibody against CD 45, commonly regarded as a pan-leukocyte marker, reacted in the bovine embryo with different types of blood cells, as well as with presumptive vasculogenetic cells and a subpopulation of putative primordial germ cells. CD 61 immunoreaction proved to be a useful tool for demonstrating megakaryocytopoiesis in the embryonic liver, in addition to the lumen of blood vessels and the mesonephros. Staining with BM-2 was restricted to a single population of medium-sized, round to oval cells, forming small groups within the parenchymal strands of the liver. Characterised furthermore by a U-shaped nucleus, this BM-2-positive cell type apparently represents a developmental stage in the granulopoietic lineage. B-lymphocytopoiesis in the bovine liver was detected with antibodies directed against WC-4 and IgM, but not until day 58 post-insemination. Using antibodies to CD 14, no positive results could be obtained in embryonic tissues, although anti-CD 14-positive macrophages were easily recognised in lymph nodes of adult bovines. The antibody against CD 68, however, identified two populations of primitive macrophages in our samples. One population was located in parenchymal strands of the embryonic liver, probably acting as nursing cells for haematopoietic foci, and the other was observed intravasally in the sinusoids of the liver, most probably representing primitive Kupffer cells.

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Section: Lineage Markersmentioning

confidence: 54%

Histochemical in situ identification of bovine embryonic blood cells reveals differences to the adult haematopoietic system and suggests a close relationship between haematopoietic stem cells and primordial germ cells

Kritzenberger

Wrobel

2004

Histochemistry and Cell Biology

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“…For the generation of bovine monocyte‐derived DC, PBMC were also derived as described above, and DC were generated from PBMC as described elsewhere 27 but with minor modifications. PBMC were incubated with a monoclonal antibody to the bovine CD14 molecule 28 followed by goat anti‐mouse immunoglobulin G1 (IgG1)‐coated super‐paramagnetic particles (Miltenyi‐Biotech, Bergisch Gladbach, Germany), and labelled cells were isolated from a MidiMacs column (Miltenyi‐Biotech) according to the manufacturer's instructions. The purity of the cells was evaluated by flow cytometry and shown in each case to be > 98%.…”

Section: Methodsmentioning

confidence: 99%

Differential production of cytokines, reactive oxygen and nitrogen by bovine macrophages and dendritic cells stimulated with Toll‐like receptor agonists

et al. 2003

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Summary Toll‐like receptors (TLR) have been described as partially sharing signalling pathways but showing unique ligand specificity and tissue distribution. Here, the response of bovine macrophages (Mφ) and dendritic cells (DC), both derived from monocytes, was compared by exposing them to the TLR‐specific ligands lipopolysaccharide, poly(I:C)‐double‐stranded RNA, and CpG‐DNA, as well as inactivated Gram‐negative and Gram‐positive bacteria, shown to bind to TLR. The production of NO, superoxide anion, interleukin‐10 (IL‐10), IL‐12 and tumour necrosis factor (TNF) was determined. Compared to monocytes, Mφ expressed more TLR2 and similar levels of TLR4 mRNA transcripts, as analysed by quantitative polymerase chain reaction, whereas DC expressed reduced amounts. Although both DC and Mφ recognized the TLR ligands, dramatic differences were seen in their reaction pattern to them. Both cell types responded with the production of TNF, but DC produced more IL‐12, whereas Mφ produced more IL‐10, regardless of the TLR agonist used. Co‐stimulation with interferon‐γ influenced the amount of cytokine production, but did not alter the cell type‐specific response pattern. Compared to Mφ, DC produced > 10 times less NO upon triggering with TLR ligands. In addition, DC produced superoxide anion to opsonized and non‐opsonized zymosan, but not to phorbol 12‐myristate 13‐acetate, a response pattern confirmed for human Mφ and DC, respectively. Different protein kinase C isoforms and extracellular signal‐regulated kinase patterns were detected in cell lysates of resting and stimulated Mφ and DC. Collectively, our results point to profound differences in pathogen‐derived signal–response coupling occurring commensurate with distinct functions carried out by Mφ or DC.

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“…In ruminants, mCD14 is a specific surface marker for monocytes and macrophages (Sopp et al 1996), but its role has been insufficiently investigated.…”

Section: Discussionmentioning

confidence: 99%

Relationship between polymorphism in the tumour necrosis factor-alpha gene and selected indices and cell subpopulations in naturally bovine leukaemia virus-infected and healthy cows

Bojarojć-Nosowicz¹,

Kaczmarczyk²,

Jastrzębska³

2018

Vet. Med. - Czech

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Identification of bovine CD14

Cited by 44 publications

References 6 publications

Histochemical in situ identification of bovine embryonic blood cells reveals differences to the adult haematopoietic system and suggests a close relationship between haematopoietic stem cells and primordial germ cells

Histochemical in situ identification of bovine embryonic blood cells reveals differences to the adult haematopoietic system and suggests a close relationship between haematopoietic stem cells and primordial germ cells

Differential production of cytokines, reactive oxygen and nitrogen by bovine macrophages and dendritic cells stimulated with Toll‐like receptor agonists

Relationship between polymorphism in the tumour necrosis factor-alpha gene and selected indices and cell subpopulations in naturally bovine leukaemia virus-infected and healthy cows

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