Identification of bibenzyls and evaluation of imitative wild planting techniques in Dendrobium officinale by HPLC‐ESI‐MSⁿ

Abstract

Dendrobium officinale is a traditional Chinese herb with beneficial properties. Modern pharmacological studies show that bibenzyl is one of the antitumor active ingredients, but there is no effective quality control method for identifying ingredients. In this study, the composition of bibenzyls in Dendrobium officinale was studied by high‐performance liquid chromatography coupled with electrospray ionization multistage mass spectrometry (HPLC‐ESI‐MSn). A total of nine isolated bibenzyls and their glycosides, 22 bis (bibenzyls), and two phenylpropanol bibenzyl derivatives were identified. The results of HPLC characteristic chromatogram analysis and statistical analysis showed that the relative content of bibenzyls in wild imitation cultivation of samples had been significantly higher than that in greenhouse cultivation. In addition, the relative content of bibenzyls increased with the growth of the original plant. This study provided a scientific reference for controlling the quality of bibenzyls in Dendrobium officinale, developing the cultivation technology and improving the quality of Dendrobium officinale. Highlights HPLC‐ESI‐MS/MS method for the analysis of bibenzyls and bis (bibenzyls) in Dendrobium officinale. Easy‐to‐use method facilitating rapid measurement of large sample quantities. The method requires only small volumes of samples for the analysis. Applicable for the establishment of Chinese medicine studies and the quality control standard of Chinese herbs.

Wild imitating vs greenhouse cultivated Dendrobium huoshanense: Chemical quality differences

“…The characteristic chromatograms of all samples were shown in S1C and S1D Fig . Peaks L1~6 were determined to be lignans and peaks B1~5 were identified as bibenzyls based on their UV spectra [ 37 ]. Both compounds have maximum absorption at 215 and 270 in the UV spectrum, but the difference is that the UV spectrum of bibenzyl compounds has a higher shoulder peak at the maximum absorption of 215 nm.…”

Rapid Measurement of Total Saponins, Mannitol, and Naringenin in Dendrobium officinale by Near-Infrared Spectroscopy and Chemometrics

“…Zhang et al revealed that D. officinale supplementation can promote alcohol metabolism and alleviate alcoholic fatty liver disease [5]. An increasing body of studies has demonstrated that a variety of active compounds, including polysaccharides, flavonoids, mannitol, naringenin, and total saponins, are closely associated with the antioxidant, anti-cancer, and anti-inflammatory properties of D. officinale [6][7][8]. Due to its health benefits and therapeutic properties, D. officinale has been widely prepared into a variety of health products, such as oral liquid, lozenges, capsules, and so on.…”