Identification of bacterial genes required for in-vivo survival

Abstract

Genetic approaches used for in-vivo studies of bacterial pathogenesis are providing insights into how bacteria disrupt host defences and exploit host molecules for their own advantage.Signature tagged mutagenesis (STM) provides a means of identifying the genes involved in the process of infection, particularly those genes that are important for bacterial proliferation invivo. In this review, the application of STM to the understanding of bacterial pathogenesis and findings from work on three human pathogens, Salmonella typhimurium, Mycobacterium tuberculosis and Neisseria meningitidis, are discussed. The next challenge is to understand how these and other genes influence the infective process at the molecular and cellular levels and to design novel interventions to block the progression of disease.

Signature tagged mutagenesis in the functional genetic analysis of gastrointestinal pathogens

“…In mice the bacterium spreads to cells of the reticulo-endothelial (RE) system and therefore it replicates human typhoid fever. 73 S. typhimurium was the first bacterium used in an STM screen. 1 It was chosen for proof-in-principle of STM because of its excellent genetic systems and well validated animal models.…”

“…1 It was chosen for proof-in-principle of STM because of its excellent genetic systems and well validated animal models. 1,73 The original STM screen identified a novel pathogenicity island, SPI-2 74 . SPI-2 was only identified due to its role in disseminated infection.…”