Identification of an interferon gamma homologue in Fugu, Takifugu rubripes

Zou, Jun; Yoshiura, Yasutoshi; Dijkstra, Johannes M.; Sakai, Masahiro; Ototake, Mitsuru; Secombes, Chris J.

doi:10.1016/j.fsi.2004.04.015

Cited by 154 publications

(79 citation statements)

References 21 publications

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“…Therefore, we believe the present NO response is due to innate rather than classical activation. Recently, the first fish IFN-␥ sequences were identified and characterized (38,39) and, in time, production of recombinant fish IFN-␥ might allow for a further distinction between innate and classical activation of fish macrophages.…”

Section: Discussionmentioning

confidence: 99%

Head Kidney-Derived Macrophages of Common Carp (Cyprinus carpio L.) Show Plasticity and Functional Polarization upon Differential Stimulation

Joerink

Ribeiro

Stet

et al. 2006

The Journal of Immunology

135

109

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Cells from the myeloid lineage are pluripotent. To investigate the potential of myeloid cell polarization in a primitive vertebrate species, we phenotypically and functionally characterized myeloid cells of common carp (Cyprinus carpio L.) during culture. Flow cytometric analysis, Ab labeling of cell surface markers, and light microscopy showed the presence of a major population of heterogeneous macrophages after culture. These head kidney-derived macrophages can be considered the fish equivalent of bone marrow-derived macrophages and show the ability to phagocytose, produce radicals, and polarize into innate activated or alternatively activated macrophages. Macrophage polarization was based on differential activity of inducible NO synthase and arginase for innate and alternative activation, respectively. Correspondingly, gene expression profiling after stimulation with LPS or cAMP showed differential expression for most of the immune genes presently described for carp. The recently described novel Ig-like transcript 1 (NILT1) and the CXCR1 and CXCR2 chemokine receptors were up-regulated after stimulation with cAMP, an inducer of alternative activation in carp macrophages. Up-regulation of NILT1 was also seen during the later phase of a Trypanosoma carassii infection, where macrophages are primarily alternatively activated. However, NILT1 could not be up-regulated during a Trypanoplasma borreli infection, a model for innate activation. Our data suggest that NILT1, CXCR1, and CXCR2 could be considered markers for alternatively activated macrophages in fish.

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Section: Discussionmentioning

confidence: 99%

Head Kidney-Derived Macrophages of Common Carp (Cyprinus carpio L.) Show Plasticity and Functional Polarization upon Differential Stimulation

Joerink

Ribeiro

Stet

et al. 2006

The Journal of Immunology

135

109

View full text Add to dashboard Cite

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“…There is no significant sequence similarity between IFN-␥ and the interferons of the ␣͞␤ group and the similarities in function (all interferons induce resistance of cells to virus infection and all act as cytokines regulating cellular activities) and in tertiary structure (the presence of a bundle of ␣-helices) could be the result of either divergence or convergence. Although orthologs of IFN-␥ exist in bony fishes (58), no corresponding sequence could be found in either the agnathan transcriptome (6,8) or the urochordate genome (45). The gene might have therefore arisen during the agnathan-gnathostome transition as an evolutionary novelty.…”

Section: Signaling Moleculesmentioning

confidence: 99%

The descent of the antibody-based immune system by gradual evolution

Klein

Nikolaidis

2004

Proc. Natl. Acad. Sci. U.S.A.

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The antibody-based immune system (AIS) is one of many means by which organisms protect themselves against pathogens and parasites. The AIS is present in jawed vertebrates (gnathostomes) but absent in all other taxa, including jawless vertebrates (agnathans). We argue that the AIS has been assembled from elements that have primarily evolved to serve other functions and incorporated existing molecular cascades, resulting in the appearance of new organs and new types of cells. Some molecules serving other functions have been appropriated by the AIS, whereas others have been modified to serve new functions, either after the duplication of their encoding genes or through the acquisition of an additional function without gene duplication. A few molecules may have been created de novo. The deployment and integration of the ready-made elements gives the impression of a sudden origin of the AIS. In reality, however, the AIS is an example of an organ system that has evolved gradually through a series of small steps over an extended period.gnathostomes ͉ agnathans ͉ immunoglobulin ͉ lymphocyte ͉ thymus

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“…Homologues of the IFN␥ have been identified in a number of bony fish (teleosts) including zebrafish (17), Japanese pufferfish (18), trout (19), Atlantic salmon (20), catfish (21), common carp (22), and goldfish (23). Of these fish species, zebrafish, catfish, common carp and goldfish have two isoforms of IFN␥, which differ markedly within each species in both sequence homology and expression in different tissues (17,21,22).…”

mentioning

confidence: 99%

Comparison of Macrophage Antimicrobial Responses Induced by Type II Interferons of the Goldfish (Carassius auratus L.)

Grayfer

García‐García

Belosevic

2010

Journal of Biological Chemistry

104

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Unlike mammals, bony fish have two type II interferons, IFN␥ and IFN␥rel, whose pro-inflammatory functions have not been fully characterized. To elucidate the distinct roles of these type II interferons of bony fish, we examined the effects of recombinant goldfish (rg) IFN␥ and IFN␥rel on the macrophage antimicrobial responses, immune gene expression, and their signaling pathways. Our findings indicate that rgIFN␥ and rgIFN␥rel possess unique capacities to mediate each of the above processes. Q-PCR analysis revealed similar expression of both cytokines in tissues and immune cell populations of the goldfish, although IFN␥ mRNA levels were generally higher in most tissues and cell types. Whereas rgIFN␥ had long-lasting effects on the priming of goldfish monocyte ROI production, the rgIFN␥rel had relatively short-lived ROI priming potential and eventually down-regulated the priming of ROI production induced by rgIFN␥ or rgTNF␣2. Whereas rgIFN␥ induced relatively modest phagocytic and nitric oxide responses of goldfish macrophages, rgIFN␥rel induced significantly higher phagocytosis, iNOSA and iNOSB gene expression and nitric oxide production compared with rgIFN␥. The rgIFN␥ and rgIFN␥rel induced different gene expression profiles in goldfish monocytes. These differences included significantly higher induction of TNF␣2, CXCL8, ceruloplasmin, and interferon regulatory factor (IRFs) expression after activation of monocytes with rgIFN␥rel. The rgIFN␥rel was more abundant in whole cell lysates compared with rgIFN␥. Both cytokines induced the phosphorylation of Stat1, while the nuclear localization of Stat1 was only observed following treatment of monocytes with rgIFN␥. Our findings suggest the presence of functional segregation of the induction of macrophage antimicrobial functions by type II interferons of bony fish.

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Identification of an interferon gamma homologue in Fugu, Takifugu rubripes

Cited by 154 publications

References 21 publications

Head Kidney-Derived Macrophages of Common Carp (Cyprinus carpio L.) Show Plasticity and Functional Polarization upon Differential Stimulation

Head Kidney-Derived Macrophages of Common Carp (Cyprinus carpio L.) Show Plasticity and Functional Polarization upon Differential Stimulation

The descent of the antibody-based immune system by gradual evolution

Comparison of Macrophage Antimicrobial Responses Induced by Type II Interferons of the Goldfish (Carassius auratus L.)

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