Identification of amino acid response element of SLC38A9 as an ATF4-binding site in porcine skeletal muscle cells

Wang, Dan; Guo, Chang-Tong; Wan, Xuebin; Guo, Kai; Niu, Hongdan; Zheng, Rong; Chai, Jin Choul; Jiang, Siwen

doi:10.1016/j.bbrc.2021.06.083

Cited by 2 publications

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“…In this study, the expression of SLC38A9 and SLC36A1 was increased with the addition of Arg or Leu after amino acid starvation (Figure 1). In our previous study, addition of Leu after amino acid starvation increased the mRNA expression of SLC38A9 in porcine skeletal muscle cells [29]. SLC38A9 and SLC36A1 may regulate mTORC1 activity in an amino acid-dependent manner [15].…”

Section: Discussionmentioning

confidence: 81%

“…After pretreatment without serum for 15 h and without amino acids for 3 h, cells were cultured for 10 min in the special medium (Supplemental Table S1) including the no amino acids group (−AA), the only Arg group (+Arg), the only Leu group (+Leu), and the only Gln group (+Gln). All of the special medium treatments contained the physiological concentration of amino acids found in pig plasma [27][28][29][30].…”

Section: Special Cell Culture Medium Treatmentmentioning

confidence: 99%

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Insights into the Interaction of Lysosomal Amino Acid Transporters SLC38A9 and SLC36A1 Involved in mTORC1 Signaling in C2C12 Cells

Wang

Wan

et al. 2021

Biomolecules

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Amino acids are critical for mammalian target of rapamycin complex 1 (mTORC1) activation on the lysosomal surface. Amino acid transporters SLC38A9 and SLC36A1 are the members of the lysosomal amino acid sensing machinery that activates mTORC1. The current study aims to clarify the interaction of SLC38A9 and SLC36A1. Here, we discovered that leucine increased expressions of SLC38A9 and SLC36A1, leading to mTORC1 activation. SLC38A9 interacted with SLC36A1 and they enhanced each other’s expression levels and locations on the lysosomal surface. Additionally, the interacting proteins of SLC38A9 in C2C12 cells were identified to participate in amino acid sensing mechanism, mTORC1 signaling pathway, and protein synthesis, which provided a resource for future investigations of skeletal muscle mass.

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Section: Discussionmentioning

confidence: 81%

Section: Special Cell Culture Medium Treatmentmentioning

confidence: 99%

Insights into the Interaction of Lysosomal Amino Acid Transporters SLC38A9 and SLC36A1 Involved in mTORC1 Signaling in C2C12 Cells

Wang

Wan

et al. 2021

Biomolecules

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Application of Traditional Graphic Elements Based on Fiber Bragg Grating Tactile Sensor Technology

2021

Journal of Sensors

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In today’s era, sensing technology has been very developed in the fields of machinery, medicine, safety monitoring, and so on. In order to be applicable to more fields, new sensing technologies are also evolving. Tactile and sensory states are two important indicators of human induction to external things. Based on the contact force information between fiber Bragg grating tactile sensor and human body, this paper uses a new fiber Bragg grating tactile sensing system to study the artistic elements of ink painting in modern graphic design. Subsequently, the structure and specific performances of the sensor experimental data are analyzed. With its small volume, low cost, high sensitivity, and good compatibility with the observed body, the new fiber Bragg grating tactile sensor will not be affected by external electromagnetic interference, various noise signals, complex integration, and other problems. According to the data observed by ink painters in this paper, the new tactile sensing technology of fiber Bragg grating can well control the external interference factors. In general, the technology is of great social and practical value.

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Identification of amino acid response element of SLC38A9 as an ATF4-binding site in porcine skeletal muscle cells

Cited by 2 publications

References 29 publications

Insights into the Interaction of Lysosomal Amino Acid Transporters SLC38A9 and SLC36A1 Involved in mTORC1 Signaling in C2C12 Cells

Insights into the Interaction of Lysosomal Amino Acid Transporters SLC38A9 and SLC36A1 Involved in mTORC1 Signaling in C2C12 Cells

Application of Traditional Graphic Elements Based on Fiber Bragg Grating Tactile Sensor Technology

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