Identification of a thioredoxin peroxidase gene involved in resistance to nucleopolyhedrovirus infection in Helicoverpa armigera with RNA interference

Zhang, Songdou; Shen, Zhizhang; Li, Zhen; Wu, Fengming; Zhang, Boyu; Liu, Yanjun; Zhang, Qingwen; Liu, Xiaoxia

doi:10.1016/j.jinsphys.2015.07.017

Cited by 14 publications

(17 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further analyses of Tajima's D statistics (column H in S4 Table ) and of GO functional categories (S5 Table ) of the 69 candidate genes between GX-P-HR and GX-P-LR identified NlPrx as the most likely fitness modifier gene candidate of imidacloprid resistance in N. lugens. This agrees with the function of peroxiredoxins to scavenge ROS, which can be elicited by exposure to many insecticides and plant defense allelochemicals [61,[54][55][56][66][67][68] and can reduce the fitness of insects [57,58]. This is also consistent with the findings that field-evolved imidacloprid resistance in N. lugens is mainly conferred by overexpression of the P450 genes CYP6ER1 and CYP6AY1 [40][41][42][43][44]69] and that P450s are known to be capable of generating ROS while metabolizing xenobiotics [62][63][64][69][70][71][72][73].…”

Section: Discussionsupporting

confidence: 89%

“…The significantly lower ROS level but higher NlPrx expression at 0 and 36 h after imidacloprid exposure in the high-resistant R-P population than in the low-resistant S-P population suggest that scavenging ROS is the direct phenotypic effect of the upregulation of NlPrx by the T65549 allele. The phenotypic effect of clearing ROS is not only consistent with the ROS-scavenging function of peroxiredoxins [61,66,67], significant (P < 0.05) differences are depicted by double and single asterisks (Student t test), respectively. NS, no significant difference; qRT-PCR, quantitative reverse transcription PCR; ROS, reactive oxygen species; RNAi, RNA interference.…”

Section: Discussionmentioning

confidence: 57%

See 1 more Smart Citation

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

et al. 2021

View full text Add to dashboard Cite

Chemical insecticides have been heavily employed as the most effective measure for control of agricultural and medical pests, but evolution of resistance by pests threatens the sustainability of this approach. Resistance-conferring mutations sometimes impose fitness costs, which may drive subsequent evolution of compensatory modifier mutations alleviating the costs of resistance. However, how modifier mutations evolve and function to overcome the fitness cost of resistance still remains unknown. Here we show that overexpression of P450s not only confers imidacloprid resistance in the brown planthopper, Nilaparvata lugens, the most voracious pest of rice, but also leads to elevated production of reactive oxygen species (ROS) through metabolism of imidacloprid and host plant compounds. The inevitable production of ROS incurs a fitness cost to the pest, which drives the increase or fixation of the compensatory modifier allele T65549 within the promoter region of N. lugens peroxiredoxin (NlPrx) in the pest populations. T65549 allele in turn upregulates the expression of NlPrx and thus increases resistant individuals’ ability to clear the cost-incurring ROS of any source. The frequent involvement of P450s in insecticide resistance and their capacity to produce ROS while metabolizing their substrates suggest that peroxiredoxin or other ROS-scavenging genes may be among the common modifier genes for alleviating the fitness cost of insecticide resistance.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 57%

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In insects, TPXs are essential for the elimination of excess ROS caused by a variety of environmental stressors (Lu & Holmgren, ; Perkins et al., ). These enzymes play a pivotal role in protecting against cellular oxidative damage induced by temperature variation, toxic heavy metals, insecticides, UV radiation, and microbial invasion (Wan et al., ; Yu et al., ; Zhang & Lu, ; Zhang et al., ). TPXs are required for successful ingestion and reproduction because they regulate the H 2 O 2 concentration in blood‐feeding insect species such as mosquitoes and ticks (Kusakisako et al., ; Peterson & Luckhart, ).…”

Section: Discussionmentioning

confidence: 99%

“…In insects, expression of Tpx s differs in various developmental stages. In H. armigera , transcripts of a Tpx gene were most abundant in the fifth‐instar larval stage, but less abundant in early‐instar larval stages, pupae, and adults (Zhang et al., ). By contrast, in the Indian meal moth, Plodia interpunctella , a Tpx was found to be mainly expressed in both egg and adult stages (Shafeeq, Cha, Shim, & Lee, ).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, in the Chinese honeybee, Apis cerana cerana , expression of Tpx genes is upregulated by various stressors such as UV light, HgCl 2 , H 2 O 2 , insecticides, and thermal challenge, and TPX enzymes display ROS scavenging ability and thus protect insects against oxidative damage (Yan et al., ; Yu, Kang, Meng, Guo, & Xu, ). Furthermore, knockdown of Tpx genes in the common cutworm, Spodoptera litura , the cotton bollworm, Helicoverpa armigera , and the pea aphid, Acyrthosiphon pisum , by RNA interference (RNAi) accelerates insect death following fungal and viral invasion (Chen et al., ; Zhang & Lu, ; Zhang et al., ). Moreover, silencing a Tpx gene in the tick species, Haemaphysalis longicornis , leads to a significant decrease in body weight, egg weight, and hatching rate (Kusakisako et al., ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of a typical 2‐Cys thioredoxin peroxidase from the Asiatic rice borer Chilo suppressalis and its role in oxidative stress

Cao

Yang

et al. 2018

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

In insects, thioredoxin peroxidase (TPX) plays an important role in protecting against oxidative damage. However, studies on the molecular characteristics of TPXs in the Asiatic rice borer, Chilo suppressalis, are limited. In this work, a cDNA sequence (CsTpx3) encoding a TPX was identified from C. suppressalis. The deduced CsTPX3 protein shares high sequence identity and two positionally conserved cysteines with orthologs from other insect species, and was classified as a typical 2-Cys TPX. CsTpx3 was expressed most highly during the fifth-instar larval stage, and transcripts were most abundant in the midgut. Recombinant CsTPX3 protein expressed in Escherichia coli displayed the expected peroxidase activity by removing H O . Furthermore, CsTPX3 protected DNA from oxidative damage, and E. coli cells overexpressing CsTPX3 exhibited long-term resistance to oxidative stress. Exposure to various oxidative stressors, such as cold (8°C), heat (35°C), bacteria (E. coli), and two insecticides (chlorpyrifos and lambda-cyhalothrin), significantly upregulated transcription of CsTpx3. However, exposure to abamectin had no such effect. Our results provide valuable information for future studies on the antioxidant mechanism in this insect species.

show abstract

A thioredoxin peroxidase protects Pieris rapae from oxidative stress induced by chlorantraniliprole exposure

Zhao

Cao

Wang

et al. 2022

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Chlorantraniliprole (CAP) is an insecticide widely used to control the small white butterfly (SWB), Pieris rapae. Exposure to CAP can cause oxidative injury in SWB; however, it is unclear if antioxidant enzymes are involved in the defense process. In this study, a thioredoxin peroxidase (PrTPX1) gene was identified from SWB by using a homology search method. The gene encoded a 195 amino‐acid PrTPX1 protein. Sequence characteristics and phylogenetic analysis indicated that PrTPX1 was a typical “2‐Cys” TPX, and the PrTPX1 gene consisted of four exons and three introns. Reverse transcription‐quantitative polymerase chain reaction analysis indicated that the messenger RNA levels of PrTPX1 were highest in third‐, fourth‐ and fifth‐instar larval stages and in the larval midgut. Treatment with sublethal doses (LD20 and LD50) of CAP for 6, 12, 18, and 24 h resulted in increased H2O2 concentration in SWB larvae, indicating insecticide‐induced oxidative stress. The transcriptional levels of PrTPX1 were significantly enhanced in larvae exposed to CAP. Recombinant PrTPX1 protein was expressed in Escherichia coli. Enzymatic assay revealed that the protein displayed antioxidant activity and was able to protect against oxidative challenge. These results indicated that PrTPX1 plays an important role in oxidative stress responses and may contribute to the CAP tolerance in SWB.

show abstract

Identification of a thioredoxin peroxidase gene involved in resistance to nucleopolyhedrovirus infection in Helicoverpa armigera with RNA interference

Cited by 14 publications

References 45 publications

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

Molecular characterization of a typical 2‐Cys thioredoxin peroxidase from the Asiatic rice borer Chilo suppressalis and its role in oxidative stress

A thioredoxin peroxidase protects Pieris rapae from oxidative stress induced by chlorantraniliprole exposure

Contact Info

Product

Resources

About