Identification of a Soluble NADPH Oxidoreductase (BmNOX) with Antiviral Activites in the Gut Juice of<i>Bombyx mori</i>

Selot, Ruchita; Kumar, Virendra; Shukla, Sunita J.; Chandra-Kuntal, Kumar; Mopidevi, Brahmaraju; Dandin, S. B.; Laloraya, Malini; Kumar, Pradeep

doi:10.1271/bbb.60450

Cited by 51 publications

(34 citation statements)

References 21 publications

(24 reference statements)

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“…It is overexpressed in the intestinal juice of disease-resistant multivoltine races. It possesses anti-BmNPV activity which attenuates the ability of the viral particles to infect BmN cells in vitro [20].…”

Section: Antiviral Activitymentioning

confidence: 99%

Bioactive Constituents of the Silk Worm Bombyx mori

Xia

Fang

et al. 2013

Antitumor Potential and Other Emerging Medicinal Properties of Natural Compounds

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Silkworm is economically important and also has a large number of activities encompassing anticancer, antibacterial, antibacterial, antiviral, antihypertensive, angiotensin-converting enzyme activity, hepatoprotective, superoxide dismutase enzyme activity, free radical scavenging and tyrosinase inhibitory, antidiabetic, antityrosinase, cognition enhancing, alternative complement pathwayinhibiting, carotenoid binding, chymotrypsin inhibiting, and chitinase activities. These activities may be beneficial to human health.

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Section: Antiviral Activitymentioning

confidence: 99%

Bioactive Constituents of the Silk Worm Bombyx mori

Xia

Fang

et al. 2013

Antitumor Potential and Other Emerging Medicinal Properties of Natural Compounds

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“…After infection with BmNPV per os, the virus is released into the midgut, where it is exposed to midgut juices containing some proteins with anti-BmNPV activity [5][6][7][8]. Bmlipase-1, a 29-kDa protein, first purified by Ponnuvel et al [5] from the digestive juice of silkworm larvae, which had lipase activity, is representative of such proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Other proteins, such as B. mori serine protease-2 (BmSP-2) [6] and BmNox [7,8], were also purified from the gut juice of silkworm larvae and showed strong antiviral activity against BmNPV.…”

Section: Introductionmentioning

confidence: 99%

Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms

et al. 2012

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Transgenic technology is a powerful tool for improving disease-resistant species. Bmlipase-1, purified from the midgut juice of Bombyx mori, showed strong antiviral activity against B. mori nucleopolyhedrovirus (BmNPV). In an attempt to create an antiviral silkworm strain for sericulture, a transgenic vector overexpressing the Bmlipase-1 gene was constructed under the control of a baculoviral immediate early-1 (IE1) promoter. Transgenic lines were generated via embryo microinjection. The mRNA level of Bmlipase-1 in the midguts of the transgenic line was 27.3 % higher than that of the non-transgenic line. After feeding the silkworm with different amounts of BmNPV, the mortality of the transgenic line decreased to approximately 33 % compared with the non-transgenic line when the virus dose was 10(6) OB/larva. These results imply that overexpressing endogenous antiviral genes can enhance the antiviral resistance of silkworms.

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“…mori larval midgut, such as lipase (Ponnuvel et al, 2003), serine protease (Nakazawa et al, 2004) and NADPH oxidoreductase (Selot et al, 2007) have been reported to exhibit antiviral activity against BmNPV infection. Many other antiviral factors may be involved in the complex immune responses of insects, but these remain undefined.…”

Section: Discussionmentioning

confidence: 99%

Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

Lekha¹,

Vijayagowri²,

Sasibhushan³

et al. 2014

International Journal of Industrial Entomology

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The variation in the level of immune response related gene expression in silkworm, Bombyx mori following infection with Bombyx mori nucleopolyhedrovirus (BmNPV) was analyzed at different time intervals. The occlusion bodies of BmNPV orally inoculated to the two most divergent silkworm races viz., Sarupat (resistant to BmNPV infection) and CSR2 (susceptible to BmNPV infection) were subjected to oral BmNPV inoculation. The expression profile of gp41 gene of BmNPV in the Sarupat and CSR2 races revealed that the virus could invade the midguts of both susceptible and resistant races. However, its multiplication was significantly less in the midgut of resistant race, while, in the susceptible race, the viral multiplication reached maximum level within 12 h. These findings indicate that potential host genes are involved in the inhibition of viral multiplication within larval midgut.

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Identification of a Soluble NADPH Oxidoreductase (BmNOX) with Antiviral Activites in the Gut Juice ofBombyx mori

Cited by 51 publications

References 21 publications

Bioactive Constituents of the Silk Worm Bombyx mori

Bioactive Constituents of the Silk Worm Bombyx mori

Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms

Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

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