Identification of a Serratia marcescens Clinical Isolate with Multiple Quinolone Resistance Mechanisms from China

Yang, Haifei; Cheng, Jun; Hu, Lifen; Ye, Ying; Li, Jiabin

doi:10.1128/aac.00070-12

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…7 Therapeutics against P. aeruginosa are increasingly limited due to the continued emergence and spreading of antibiotic resistant strains causing high mortality. 8 At the same time, multidrug-resistant strains of S. marcescens have been isolated from both clinical and environmental settings 9 , suggesting that antibiotic usage should be restricted and the alternative therapeutic approach should be implemented to treat these infections. Both S. marcescens and P. aeruginosa control their virulence factor production including biofilm formation using QS system.…”

Section: Introductionmentioning

confidence: 99%

Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa

et al. 2017

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Antibiotic resistance has become a serious global threat to public health; therefore, improved strategies and structurally novel antimicrobials are urgently needed to combat infectious diseases. Here we report a new type of highly potent 4-aminoquinoline derivatives as quorum sensing inhibitors in Serratia marcescens and Pseudomonas aeruginosa, exhibiting weak bactericidal activities (minimum inhibitory concentration (MIC) > 400 μM). Through detailed structure-activity study, we have identified 7-Cl and 7-CF substituted N-dodecylamino-4-aminoquinolines (5 and 10) as biofilm formation inhibitors with 50% biofilm inhibition at 69 μM and 63 μM in S. marcescens and P. aeruginosa, respectively. These two compounds, 5 and 10, are the first quinoline derivatives with anti-biofilm formation activity reported in S. marcescens. Quantitative structure-activity relationship (QSAR) analysis identified structural descriptors such as Wiener indices, hyper-distance-path index (HDPI), mean topological charge (MTC), topological charge index (TCI), and log D(o/w) as the most influential in biofilm inhibition in this bacterial species. Derivative 10 is one of the most potent quinoline type inhibitors of pyocyanin production described so far (IC = 2.5 μM). While we have demonstrated that 5 and 10 act as Pseudomonas quinolone system (PQS) antagonists, the mechanism of inhibition of S. marcescens biofilm formation with these compounds remains open since signaling similar to P. aeruginosa PQS system has not yet been described in Serratia and activity of these compounds on acylhomoserine lactone (AHL) signaling has not been detected. Our data show that 7-Cl and 7-CF substituted N-dodecylamino-4-aminoquinolines present the promising scaffolds for developing antivirulence and anti-biofilm formation agents against multidrug-resistant bacterial species.

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Section: Introductionmentioning

confidence: 99%

Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa

et al. 2017

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“…Most S. marcescens strains are reported as having resistance to several antibiotics (Yang et al 2012). We found that the strain we used here was resistant to most of the tested antibiotics (Table 2) and intermediate to ciprofloxacin and norfloxacin (Table 2).…”

Section: Antibiotic Resistancementioning

confidence: 65%

“…Antibiotics play an important role in the battle against infections caused by S. marcescens. However, it was reported that S. marcescens strains were resistant to a large number of antibiotics, including β-lactam, aminoglycoside and fluoroquinolone (Ishii et al 2012;Yang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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In vitrosynergistic effects of fisetin and norfloxacin against aquatic isolates ofSerratia marcescens

Dong

Ruan

et al. 2015

FEMS Microbiology Letters

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Serratia marcescens is a common pathogenic bacterium that can cause infections in both humans and animals. It can cause a range of diseases, from slight wound infections to life-threatening bacteraemia and pneumonia. The emergence of antimicrobial resistance has limited the treatment of the diseases caused by the bacterium to a great extent. Consequently, there is an urgent need to develop novel antimicrobial strategies against this pathogen. Synergistic strategy is a new approach to treat the infections caused by drug-resistant bacteria. In this paper, we isolated and identified the first multi-resistant pathogenic Serratia marcescens strain from diseased soft-shelled turtles (Pelodiscus sinensis) in China. We then performed a checkerboard assay; the results showed that out of 10 tested natural products fisetin had synergistic effects against S. marcescens when combined with norfloxacin. The time-kill curve assay further confirmed the results of the checkerboard assay. We found that this novel synergistic effect could significantly reduce the dosage of norfloxacin against S. marcescens.

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Private and well drinking water are reservoirs for antimicrobial resistant bacteria

Alawi,

Smyth,

Drissner

et al. 2024

npj Antimicrob Resist

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Water quality testing does not recognise antimicrobial resistance (AMR) and is often limited to indicators of faecal contamination Escherichia coli and Enterococcus species. In Europe, data on AMR in drinking water is scarce. In Ireland, as in many countries, household drinking water is supplied via mains or via private wells or water schemes. Using citizen science, we identified Irish private drinking water supplies as reservoirs of antimicrobial resistant bacteria (ARB). Gram-negative (n = 464) and Gram-positive (n = 72) bacteria were isolated. We identified instances of potentially opportunistic ARB such as Enterobacter cloacae, Acinetobacter baumannii and Enterococcus species. We report reservoirs of multidrug resistance in Enterococcus casseliflavus, E. cloacae, E. coli, Stenotrophomonas maltophilia, and Serratia rubidaea. We also identified linezolid-resistant Enterococcus in Irish drinking water. Linezolid is a last-resort antibiotic used to treat vancomycin-resistant Enterococcus sp. Additionally, we identified mobile AMR in three water samples, two of which were carried on IncF group, one on IncQ and five on Col-like plasmids. Our work suggests that private drinking water is a potential sink and source of AMR pathogens. This highlights a value of drinking water surveillance in a One Health framework as the surveillance would provide information regarding the movement and persistence of ARB and ARGs that are able to survive in drinking water and subsequently have the opportunity to be mobilised through humans; linking the environment to the human and potentially threatening human health.

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Identification of a Serratia marcescens Clinical Isolate with Multiple Quinolone Resistance Mechanisms from China

Cited by 3 publications

References 15 publications

Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa

Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa

In vitrosynergistic effects of fisetin and norfloxacin against aquatic isolates ofSerratia marcescens

Private and well drinking water are reservoirs for antimicrobial resistant bacteria

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