Identification of a Novel High Yielding Source of Multipotent Adult Human Neural Crest-Derived Stem Cells

Schürmann, Martin; Brotzmann, Viktoria; Bütow, Marlena; Greiner, Johannes F. W.; Höving, Anna L.; Kaltschmidt, Christian; Kaltschmidt, Barbara; Sudhoff, Holger

doi:10.1007/s12015-017-9797-2

Cited by 16 publications

(15 citation statements)

References 34 publications

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“…Human NCSCs can be isolated from various adult tissues, such as skin [74,75], periodontium [76], or the nasal cavity [77][78][79]. Human NCSCs can be isolated from various adult tissues, such as skin [74,75], periodontium [76], or the nasal cavity [77][78][79].…”

Section: The Role Of Sex In Neuronal Development and Neural Stem Cellmentioning

confidence: 99%

“…Neural crest stem cells not only directly contribute to embryonic development, but are commonly known to reside within the organism until and during adulthood as dormant stem cells termed NCSCs. Human NCSCs can be isolated from various adult tissues, such as skin [74,75], periodontium [76], or the nasal cavity [77][78][79]. Common characteristics of isolated NCSCs particularly include their easy accessibility, expression of Nestin, p75 NTR , and the NC markers SLUG, SNAIL, TWIST, SOX9, and SOX10 as well as the ability to self-renew and formation of neurospheres in vitro (reviewed in [66].…”

Section: Adult Neural Crest-derived Stem Cells and Sex-specific Diffementioning

confidence: 99%

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Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

et al. 2019

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Sexual dimorphisms contribute, at least in part, to the severity and occurrence of a broad range of neurodegenerative, cardiovascular, and bone disorders. In addition to hormonal factors, increasing evidence suggests that stem cell‐intrinsic mechanisms account for sex‐specific differences in human physiology and pathology. Here, we discuss sex‐related intrinsic mechanisms in adult stem cell populations, namely mesoderm‐derived stem cells, neural stem cells (NSCs), and neural crest‐derived stem cells (NCSCs), and their implications for stem cell differentiation and regeneration. We particularly focus on sex‐specific differences in stem cell‐mediated bone regeneration, in neuronal development, and in NSC‐mediated neuroprotection. Moreover, we review our own recently published observations regarding the sex‐dependent role of NF‐κB‐p65 in neuroprotection of human NCSC‐derived neurons and sex differences in NCSC‐related disorders, so‐called neurocristopathies. These observations are in accordance with the increasing evidence pointing toward sex‐specific differences in neurocristopathies and degenerative diseases like Parkinson's disease or osteoporosis. All findings discussed here indicate that sex‐specific variability in stem cell biology may become a crucial parameter for the design of future treatment strategies.

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Section: The Role Of Sex In Neuronal Development and Neural Stem Cellmentioning

confidence: 99%

Section: Adult Neural Crest-derived Stem Cells and Sex-specific Diffementioning

confidence: 99%

Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

et al. 2019

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“…After neurulation, neural crest cells migrate to a broad range of target tissues within the developing organism and give rise to various cell populations cells of mesodermal and ectodermal type 32 . In addition, neural crest-derived cells also persist as adult stem cell populations within the adult body [18][19][20][21]36 . Extending the range of known NCSC-populations within the human organism, our present findings show the existence of an adult NCSC-population within the myocardium of the adult human heart auricle.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we likewise identified Nestin-positive cells in the human heart, which expressed a range of neural crest and stemness markers including SNAIL, SLUG, SOX9, TWIST, and cMYC. Notably, similar to NCSCs populations residing within the head and neck region [15][16][17][18][19] , Nestin + human cardiac NCSCs exhibited an extraordinary high differentiation capability by giving rise to cardiomyocytes but also other mesodermal and ectodermal cell types. These observations extend the commonly described differentiation ability of human CSCs, which was reported to be restricted to cardiomyocytes, smooth muscle cells and endothelial cells in vitro 4,5 .…”

Section: Discussionmentioning

confidence: 99%

“…Among the broad range of adult human stem cells, neural crest-derived stem cells (NCSCs) show an extraordinary high differentiation potential in vivo and in vitro and are therefore considered as extended multipotent and promising candidates for clinical applications [15][16][17] . Although NCSCs can be found in various tissues of the adult human body [18][19][20][21] , a neural crest origin of CSCs has only been described in the murine heart 22,23 .…”

Section: Introductionmentioning

confidence: 99%

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Identification of a human adult cardiac stem cell population with neural crest origin

Höving

Merten

Schmidt

et al. 2019

Preprint

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Cardiovascular diseases are the major cause of death worldwide, emphasizing the necessity to better understand adult human cardiac cell biology and development. Although the adult heart was considered as a terminally differentiated organ, rare populations of cardiac stem cells (CSCs) have been described so far, with their developmental origin and endogenous function still being a matter of debate.Here, we identified a Nestin + /S100 + /CD105 + /Sca1 + /cKitpopulation of CSCs in the myocardium of the adult human heart auricle. Isolated cells showed expression of characteristic neural crest-derived stem cell (NCSC) markers and kept their genetic stability during cultivation in vitro. Cultivated hCSCs efficiently gave rise to functional, beating cardiomyocytes, osteoblasts, adipocytes and neurons. Global transcriptome analysis via RNAseq showed a high similarity between the expression profiles of Nestin + /S100 + /CD105 + /Sca1 + /cKit -hCSCs and adult human NCSCs from the nasal cavity (inferior turbinate stem cells, ITSCs). In detail, 88.1 % of all genes were significantly expressed in both stem cell populations particularly including common NCSC-markers. Based on these observations, we suggest a similar developmental origin of both stem cell populations.In summary, we identified a human adult cardiac stem cell population with neural crest-origin, which may also contribute to endogenous cardiac tissue homeostasis and tissue repair in vivo.

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Neural crest-like stem cells for tissue regeneration

Soto

Ding

Wang

et al. 2021

Stem Cells Translational Medicine

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Neural crest stem cells (NCSCs) are a transient population of cells that arise during early vertebrate development and harbor stem cell properties, such as self‐renewal and multipotency. These cells form at the interface of non‐neuronal ectoderm and neural tube and undergo extensive migration whereupon they contribute to a diverse array of cell and tissue derivatives, ranging from craniofacial tissues to cells of the peripheral nervous system. Neural crest‐like stem cells (NCLSCs) can be derived from pluripotent stem cells, placental tissues, adult tissues, and somatic cell reprogramming. NCLSCs have a differentiation capability similar to NCSCs, and possess great potential for regenerative medicine applications. In this review, we present recent developments on the various approaches to derive NCLSCs and the therapeutic application of these cells for tissue regeneration.

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Identification of a Novel High Yielding Source of Multipotent Adult Human Neural Crest-Derived Stem Cells

Cited by 16 publications

References 34 publications

Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

Identification of a human adult cardiac stem cell population with neural crest origin

Neural crest-like stem cells for tissue regeneration

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