Identification of a Molecular Species in Porcine Ovarian Luteal Glutathione S-Transferase and Its Hormonal Regulation by Pituitary Gonadotropins

Keira, M.; Nishihira, Jun; Ishibashi, Teruo; Tanaka, Toshinobu; Fujimoto, S.

doi:10.1006/abbi.1994.1018

Cited by 18 publications

(11 citation statements)

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“…Our study highlighted the overexpression of different GST genes (GSTA1, GSTA2 and MGST) in LF granulosa cells. This has already been described in steroidogenically active cells (Keira et al 1994, Rabahi et al 1999. In our study, GSTA1 in situ experiments fitted in very well with the micro-array analysis for this gene.…”

Section: Discussionsupporting

confidence: 88%

In vivo gene expression in granulosa cells during pig terminal follicular development

Bonnet¹,

Cao²,

SanCristobal³

et al. 2008

Reproduction

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Ovarian antral follicular development is clearly dependent on pituitary gonadotrophins FSH and LH. Although the endocrine mechanism that controls ovarian folliculogenesis leading to ovulation is quite well understood, the detailed mechanisms and molecular determinants in the different follicular compartments remain to be clarified. The aim of this study was to identify the genes differentially expressed in pig granulosa cells along the terminal ovarian follicle growth, to gain a comprehensive view of these molecular mechanisms. First, we developed a specific micro-array using cDNAs from suppression subtractive hybridization libraries (345 contigs) obtained by comparison of three follicle size classes: small, medium and large antral healthy follicles. In a second step, a transcriptomic analysis using cDNA probes from these three follicle classes identified 79 differentially expressed transcripts along the terminal follicular growth and 26 predictive genes of size classes. The differential expression of 18 genes has been controlled using real-time PCR experiments validating the micro-array analysis. Finally, the integration of the data using Ingenuity Pathways Analysis identified five gene networks providing descriptive elements of the terminal follicular development. Specifically, we observed: (1) the down-expression of ribosomal protein genes, (2) the genes involved in lipid metabolism and (3) the down-expression of cell morphology and ion-binding genes. In conclusion, this study gives new insight into the gene expression during pig terminal follicular growth in vivo and suggested, in particular, a morphological change in pig granulosa cells accompanying terminal follicular growth. Reproduction (2008) 136 211-224

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Section: Discussionsupporting

confidence: 88%

In vivo gene expression in granulosa cells during pig terminal follicular development

Bonnet¹,

Cao²,

SanCristobal³

et al. 2008

Reproduction

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“…Furthermore, several investigations have suggested that ovarian GST is involved in steroidogenesis in various mammalian species. These include (1) the high localization of GST to the active steroid-producing cells of human ovaries throughout the menstrual cycle (Rahilly et al 1991), (2) the increase in GST activity in porcine ovarian follicles during growth and the high activity of GST in the CL (Keira et al 1994), (3) the close relationship between changes in GST activity induced by follicle-stimulating hormone and LH in porcine cultured granulosa cells and progesterone production (Keira et al 1994), (4) the high GST activity in rat ovary observed at the oestrous stage (Singh & Pandey 1996) and (5) the high expression of GST in steroidogenically active bovine follicular and luteal cells (Rabahi et al 1999). Although the significance of high activity of GST in the CL throughout pregnancy (present study) is at present unknown, it may suggest a role in the regulation of CL steroidogenesis.…”

Section: Sheep CL Antioxidant Enzymes Throughout Pregnancy 769mentioning

confidence: 99%

Antioxidant enzymatic defence systems in sheep corpus luteum throughout pregnancy

Al-Gubory¹,

Bolifraud²,

Germain³

et al. 2004

Reproduction

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The activities of copper, zinc-superoxide dismutase (SOD1), manganese SOD (SOD2), glutathione peroxidase (GPX), glutathione reductase (GSSG-R) and glutathione S-transferase (GST) were studied in sheep corpora lutea (CL) obtained on days 15, 40, 60, 80 and 128 of pregnancy. Maintained enzymatic activity of SOD1, SOD2, GPX, GSSG-R and GST were found in the sheep CL throughout pregnancy. Enzymatic activity of SOD1, GPX and GST increased significantly from day 15 to day 40 of pregnancy, and thereafter remained constant until day 128. SOD2 and GSSG-R activities were not different between any days of pregnancy examined. Apoptotic luteal cells identified by the terminal deoxynucleotidyl transferase-mediated fluoresceindUTP nick-end labelling were very rarely observed, and their incidence (less than 0.5%) was not different between days of pregnancy. These results showed that the activities of antioxidant enzymes in the sheep CL are subject to major changes during early pregnancy, suggesting that the CL of early pregnancy may be rescued from luteolysis through increasing activities of key antioxidant enzymes and inhibition of apoptosis. Maintained levels of antioxidant enzymes in the CL throughout pregnancy may be linked to reactive oxygen species continuously generated in the steroidogenically active luteal cells, and may be involved in the maintenance of luteal steroidogenic activity and cellular integrity.

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“…GSTs are involved in the intracellular transport of compounds and their delivery to sites for subsequent transformation and/or excretion (Kaplowitz 1980) and are important in detoxifying reactive metabolites. GSTs are abundant in steroid-producing ovarian cells throughout the menstrual cycle (Rahilly et al 1991), in bovine follicular and LC (Rabahi et al 1999), and increase in activity during growth of porcine ovarian follicles and CL (Keira et al 1994). GSTA1 is involved in detoxifying environmental toxins and products of oxidative stress (Ahn et al 2006) and binds to steroid hormone metabolites, which it helps transport intracellularly (Listowsky et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

Arianmanesh¹,

McIntosh²,

Lea³

et al. 2011

Journal of Endocrinology

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Progesterone (P 4 ) secreted by the corpus luteum (CL) is critical for in utero embryo survival and development, although CL proteins are key regulatory factors during the luteal phase. We, therefore, characterised protein expression patterns in ovine CL of pregnancy (days 12, 16 and 20) compared with those of controls, CL of oestrous cycle (days 12 and 16), using two-dimensional gel electrophoresis (2DE) gel-based proteomics. Proteins in 24 significantly altered spots were identified by tandem mass spectroscopy. At the time of embryo implantation (day 16), 77 spots were up-regulated and 101 spots were down-regulated in CL of pregnancy compared with regressed CL. Vimentin, lamin A/C (LMNA), [Mn] superoxide dismutase (SOD2), isocitrate dehydrogenase 1, annexin A1 and elongation factor Tu, mitochondrial (TUFM) altered during CL regression, whereas glutathione S-transferase A1, apolipoprotein A-1, myxovirus resistance protein 1, ornithine aminotransferase and enoyl-CoA hydratase, mitochondrial (ECHS1) tended to be altered during CL maintenance. biliverdin reductase B (BLVRB), FDXR, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (GNB2) and cytochrome bc1 complex subunit 1, mitochondrial (UQCRC1) showed divergent expression during CL regression and maintenance. The expression of two representative proteins, SOD2 and BLVRB, by western blot increased in CL of non-pregnant ewes on day 16 compared with that on day 12. SOD2 and BLVRB were localised in the large and small luteal cells and endothelial cells of CL over peri-implantation periods. 2DE gel and mass spectrometry have been used, for the first time, to study ovine CL function. We have identified proteins involved in key pathways, including oxidative stress, steroidogenesis, signal transduction and apoptosis, which have not previously been associated with changes occurring in the CL during the peri-implantation period. These proteins are most likely involved with mechanisms allowing the CL to produce P 4 during early pregnancy.

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Identification of a Molecular Species in Porcine Ovarian Luteal Glutathione S-Transferase and Its Hormonal Regulation by Pituitary Gonadotropins

Cited by 18 publications

References 0 publications

In vivo gene expression in granulosa cells during pig terminal follicular development

In vivo gene expression in granulosa cells during pig terminal follicular development

Antioxidant enzymatic defence systems in sheep corpus luteum throughout pregnancy

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

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