Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response

Gibson, Amanda J.; Miah, Suhel; Griebel, Philip; Brownlie, Joe; Werling, Dirk

doi:10.1016/j.dci.2011.05.002

Cited by 10 publications

(14 citation statements)

References 63 publications

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“…CD172a has been identified as pan monocyte marker of porcine monocytes [22], [23]. CD172a is also expressed at low levels on bovine [24] and porcine [25] plasmacytoid DC. Although not formally proven, CD172a-positive bovine monocyte subsets (defined by CD172a, CD14 and CD16 staining) are also distinct from blood dendritic cells, since they are negative for both CD14 and CD16 [2].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Functional Heterogeneity of Bovine Blood Monocytes

et al. 2013

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Murine and human peripheral blood monocytes are heterogeneous in size, granularity, nuclear morphology, phenotype and function. Whether and how bovine blood monocytes follow this pattern was analyzed in this study. Flow cytometrically, classical monocytes (cM) CD14+ CD16−, intermediate monocytes (intM) CD14+ CD16+ and nonclassical monocytes (ncM) CD14+ CD16+ were identified, with cM being the predominant subset (89%). cM showed a significant lower expression of CD172a, intM expressed the highest level of MHC class II molecules, and ncM were low positive for CD163. Compared to cM and intM, ncM showed a significantly reduced phagocytosis capacity, a significantly reduced generation of reactive oxygen species, and reduced mRNA expression of CXCL8, CXCL1 and IL-1β after LPS stimulation. Based on IL-1β secretion after LPS/ATP stimulation, the inflammasome could be activated in cM and intM, but not in ncM. IFNγ increased the expression of CD16 selectively on cM and induced a shift from cM into intM in vitro. In summary, bovine CD172a-positive mononuclear cells define three monocyte subsets with distinct phenotypic and functional differences. Bovine cM and intM share homologies with their human counterparts, whereas bovine ncM are not inflammatory monocytes.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and Functional Heterogeneity of Bovine Blood Monocytes

et al. 2013

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“…vates IFN-␣/␤ production via a TLR7/8-independent mechanism. Note that bovine pDCs do not express TLR8 mRNA (20).…”

Section: Ifn-␣/␤ Induction By Uv-btv Involves Tlr7-independent Andmentioning

confidence: 99%

The Double-Stranded RNA Bluetongue Virus Induces Type I Interferon in Plasmacytoid Dendritic Cells via a MYD88-Dependent TLR7/8-Independent Signaling Pathway

Ruscanu

Pascale

Bourge

et al. 2012

J Virol

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Dendritic cells (DCs), especially plasmacytoid DCs (pDCs), produce large amounts of alpha/beta interferon (IFN-α/β) upon infection with DNA or RNA viruses, which has impacts on the physiopathology of the viral infections and on the quality of the adaptive immunity. However, little is known about the IFN-α/β production by DCs during infections by double-stranded RNA (dsRNA) viruses. We present here novel information about the production of IFN-α/β induced by bluetongue virus (BTV), a vector-borne dsRNA Orbivirus of ruminants, in sheep primary DCs. We found that BTV induced IFN-α/β in skin lymph and in blood in vivo . Although BTV replicated in a substantial fraction of the conventional DCs (cDCs) and pDCs in vitro , only pDCs responded to BTV by producing a significant amount of IFN-α/β. BTV replication in pDCs was not mandatory for IFN-α/β production since it was still induced by UV-inactivated BTV (UV-BTV). Other inflammatory cytokines, including tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and IL-12p40, were also induced by UV-BTV in primary pDCs. The induction of IFN-α/β required endo-/lysosomal acidification and maturation. However, despite being an RNA virus, UV-BTV did not signal through Toll-like receptor 7 (TLR7) for IFN-α/β induction. In contrast, pathways involving the MyD88 adaptor and kinases dsRNA-activated protein kinase (PKR) and stress-activated protein kinase (SAPK)/Jun N-terminal protein kinase (JNK) were implicated. This work highlights the importance of pDCs for the production of innate immunity cytokines induced by a dsRNA virus, and it shows that a dsRNA virus can induce IFN-α/β in pDCs via a novel TLR-independent and Myd88-dependent pathway. These findings have implications for the design of efficient vaccines against dsRNA viruses.

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“…A previous study revealed that DCs were identified at 0.1%–0.7% of PBMC (Renjifo et al., 1997). Because of the low percentage of DCs in the PBMC, it was necessary to deplete the non‐DC from bovine PBMC (Gibson, Miah, Griebel, Brownlie, & Werling, 2012; Miyazawa et al., 2006; Renjifo et al., 1997; Sei, Ochoa, Bishop, Barlow, & Golde, 2014). In this study, T cells, B cells, monocytes and granulocytes were depleted from PBMC by negative selection.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method

Zhuang

Urakawa

Sato

et al. 2018

Animal Science Journal

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Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+/CD11c+/MHC (major histocompatibility complex) class II + cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a+/CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage.

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Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response

Cited by 10 publications

References 63 publications

Phenotypic and Functional Heterogeneity of Bovine Blood Monocytes

Phenotypic and Functional Heterogeneity of Bovine Blood Monocytes

The Double-Stranded RNA Bluetongue Virus Induces Type I Interferon in Plasmacytoid Dendritic Cells via a MYD88-Dependent TLR7/8-Independent Signaling Pathway

Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method

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