Identification of a GLDH-overexpressing Arabidopsis mutant and its responses to high-light stress

Zheng, Xiaoting; Zhang, X.H.; Wang, Y.Z.; Cai, Minling; Li, M.; Zhang, T.J.; Peng, Chang-Lian

doi:10.32615/ps.2019.038

Cited by 4 publications

(4 citation statements)

References 53 publications

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“…This residue has been validated to be irreversibly oxidized by H 2 O 2 , inactivating GLDH [70], and has been suggested to be responsible for regulation of GLDH activity during the early stages of heat stress produced programmed cell death [67,71]. Moreover, Arabidopsis GLDH overexpressing lines accumulated higher ascorbate levels and demonstrated higher chlorophyll fluorescence parameters after exposure to high light for 14 days, which led them to have lower sensitivity to light stress [72].…”

Section: Light Regulation Of Ascorbatementioning

confidence: 99%

Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Rosado-Souza

Fernie

Aarabi

2020

Plants

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Cell compartmentalization allows incompatible chemical reactions and localised responses to occur simultaneously, however, it also requires a complex system of communication between compartments in order to maintain the functionality of vital processes. It is clear that multiple such signals must exist, yet little is known about the identity of the key players orchestrating these interactions or about the role in the coordination of other processes. Mitochondria and chloroplasts have a considerable number of metabolites in common and are interdependent at multiple levels. Therefore, metabolites represent strong candidates as communicators between these organelles. In this context, vitamins and similar small molecules emerge as possible linkers to mediate metabolic crosstalk between compartments. This review focuses on two vitamins as potential metabolic signals within the plant cell, vitamin C (L-ascorbate) and vitamin B1 (thiamin). These two vitamins demonstrate the importance of metabolites in shaping cellular processes working as metabolic signals during acclimation processes. Inferences based on the combined studies of environment, genotype, and metabolite, in order to unravel signaling functions, are also highlighted.

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Section: Light Regulation Of Ascorbatementioning

confidence: 99%

Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Rosado-Souza

Fernie

Aarabi

2020

Plants

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“…Also, more recently, it was demonstrated that the thale cress homozygous mutant (T-DNA insertion mutant designated GLDH-236OE ), which overexpress the GLDH gene, has a significantly high content of AsA in their leaves than wild-type plants. Also, these GLDH gene-overexpressing mutant plants have more tolerance to high light due to a better capacity to eliminate reactive-oxygen species, absorb extra light, and dissipate the thermal energy ( Zheng et al., 2019 ). Together, these results corroborate that the catalytic activity of the GLDH enzyme is an essential step in the L-galactose pathway for AsA biosynthesis in plants.…”

Section: Enzymes Of the L-galactose Pathway For Asa Biosynthesismentioning

confidence: 99%

Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway

Castro

Castro²,

Cobos³

2023

Front. Plant Sci.

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Vitamin C (L-ascorbic acid, AsA) is an essential compound with pleiotropic functions in many organisms. Since its isolation in the last century, AsA has attracted the attention of the scientific community, allowing the discovery of the L-galactose pathway, which is the main pathway for AsA biosynthesis in plants. Thus, the aim of this review is to analyze the genetic and biochemical strategies employed by plant cells for regulating AsA biosynthesis through the L-galactose pathway. In this pathway, participates eight enzymes encoded by the genes PMI, PMM, GMP, GME, GGP, GPP, GDH, and GLDH. All these genes and their encoded enzymes have been well characterized, demonstrating their participation in AsA biosynthesis. Also, have described some genetic and biochemical strategies that allow its regulation. The genetic strategy includes regulation at transcriptional and post-transcriptional levels. In the first one, it was demonstrated that the expression levels of the genes correlate directly with AsA content in the tissues/organs of the plants. Also, it was proved that these genes are light-induced because they have light-responsive promoter motifs (e.g., ATC, I-box, GT1 motif, etc.). In addition, were identified some transcription factors that function as activators (e.g., SlICE1, AtERF98, SlHZ24, etc.) or inactivators (e.g., SlL1L4, ABI4, SlNYYA10) regulate the transcription of these genes. In the second one, it was proved that some genes have alternative splicing events and could be a mechanism to control AsA biosynthesis. Also, it was demonstrated that a conserved cis-acting upstream open reading frame (5’-uORF) located in the 5’-untranslated region of the GGP gene induces its post-transcriptional repression. Among the biochemical strategies discovered is the control of the enzyme levels (usually by decreasing their quantities), control of the enzyme catalytic activity (by increasing or decreasing its activity), feedback inhibition of some enzymes (GME and GGP), subcellular compartmentation of AsA, the metabolon assembly of the enzymes, and control of AsA biosynthesis by electron flow. Together, the construction of this basic knowledge has been establishing the foundations for generating genetically improved varieties of fruits and vegetables enriched with AsA, commonly used in animal and human feed.

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“…To determine the changes during AsA biosynthesis and the regeneration process, the activities of key enzymes were determined. GLDH catalyzes the final step of AsA biosynthesis and its activity is positively correlated with AsA content across diverse tissues in a species-specific manner [34,35]. Light plays important roles in the expression of GLDH [36].…”

Section: Eop-hli With a High Blue Light Proportion Increased Asa Accu...mentioning

confidence: 99%

High-Proportion Blue Light Irradiation at the End-of-Production Stage Promotes the Biosynthesis and Recycling of Ascorbate in Lettuce

Zhou,

Li,

Liu

et al. 2023

IJMS

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Ascorbate (AsA), an essential antioxidant for both plants and the human body, plays a vital role in maintaining proper functionality. Light plays an important role in metabolism of AsA in horticultural plants. Our previous research has revealed that subjecting lettuce to high light irradiation (HLI) (500 μmol·m−2·s−1) at the end-of-production (EOP) stage effectively enhances AsA levels, while the optimal light quality for AsA accumulation is still unknown. In this study, four combinations of red (R) and blue (B) light spectra with the ratio of 1:1 (1R1B), 2:1 (2R1B), 3:1 (3R1B), and 4:1 (4R1B) were applied to investigate the biosynthesis and recycling of AsA in lettuce. The results demonstrated that the AsA/total-AsA content in lettuce leaves was notably augmented upon exposure to 1R1B and 2R1B. Interestingly, AsA levels across all treatments increased rapidly at the early stage (2–8 h) of irradiation, while they increased slowly at the late stage (8–16 h). The activity of L-galactono-1,4-lactone dehydrogenase was augmented under 1R1B treatment, which is pivotal to AsA production. Additionally, the activities of enzymes key to AsA cycling were enhanced by 1R1B and 2R1B treatments, including ascorbate peroxidase, dehydroascorbate reductase, and monodehydroascorbate reductase. Notably, hydrogen peroxide and malondialdehyde accumulation increased dramatically following 16 h of 1R1B and 2R1B treatments. In addition, although soluble sugar and starch contents were enhanced by EOP-HLI, this effect was comparatively subdued under the 1R1B treatment. Overall, these results indicated that AsA accumulation was improved by irradiation with a blue light proportion of over 50% in lettuce, aligning with the heightened activities of key enzymes responsible for AsA synthesis, as well as the accrual of hydrogen peroxide. The effective strategy holds the potential to enhance the nutritional quality of lettuce while bolstering its antioxidant defenses.

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Identification of a GLDH-overexpressing Arabidopsis mutant and its responses to high-light stress

Cited by 4 publications

References 53 publications

Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway

High-Proportion Blue Light Irradiation at the End-of-Production Stage Promotes the Biosynthesis and Recycling of Ascorbate in Lettuce

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