Identification of 21 novel S-RNase alleles and determination of S-genotypes in 66 loquat (Eriobotrya) accessions

Yang, Qin; Fu, Yan; Wang, Yongqing; Lun-pei, Liu; Li, Xingyuan; Peng, Shushi

doi:10.1007/s11032-018-0800-7

Cited by 5 publications

(8 citation statements)

References 40 publications

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“…ncbi.nlm.nih.gov/ Blast.cgi) using BLASTn and BLASTx to identify homologous genes and putative intron sequences. The deduced protein sequences between the C1 and the C5 conserved regions were obtained, and the structures were analyzed using DNAMAN (Lynnon Biosoft, San Ramon, CA) and compared with available E. japonica sequences on GenBank (Yang et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…The loquat is an evergreen rosaceous fruit tree that is native to southeastern China, but is cultivated worldwide in more than 30 countries, where it grows predominantly in subtropical regions experiencing mild temperature (Yang et al, 2018). It flowers from late autumn to early winter, the fruit flesh is soft and succulent and rich in amino acids and microelements, and its taste makes it a popular choice among consumers .…”

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confidence: 99%

“…It flowers from late autumn to early winter, the fruit flesh is soft and succulent and rich in amino acids and microelements, and its taste makes it a popular choice among consumers . It also has ornamental significance and is grown for its use in the treatment of lungrelated diseases, including cough, asthma, and chronic bronchitis, and as a result, it has been cultivated for more than 2000 years (Kikuchi et al, 2014;Yang et al, 2018;Zhang et al, 2015). Nevertheless, there are 30 species of loquats and only E. japonica was a cultivated species with narrow genetic diversity; when used as parents, several excellent varieties have been obtained by conventional cross breeding (Dong et al, 2008;Zheng, 2007).…”

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confidence: 99%

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Cut-style Pollination Can Effectively Overcome Prefertilization Barriers of Distant Hybridization in Loquat

Yang¹,

Fu²

2020

horts

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Loquat [Eriobotrya japonica (E. japonica)], a small genus of the subtribe Malinae that consists of ≈30 species, is an evergreen rosaceous fruit tree that is native to southeastern China, and some wild species that possess novel, favorable traits have excellent breeding potential. For example, Eriobotrya bengalensis blooms in late spring and ripens in early autumn in Guizhou Province, China, which prevents cold injury in winter by breeding spring-flowering cultivars using the special characters. Therefore, in the present study, the pollination treatments of cut-style pollination were evaluated that may promote successful distant hybridization in Eriobotrya japonica ‘Dawuxing’ × Eriobotrya deflexa and E. japonica ‘Dawuxing’ × E. bengalensis. The results indicated that the impairment of the pollen tube growth in the upper third of the style after pollen germination is an important factor leading to the failure of distant hybridization between the species tested in E. japonica, and that cut-style pollination can effectively overcome prefertilization barriers of the distant hybridization combination. Furthermore, the results of allele-specific polymerase chain reaction (AS-PCR) showed that S-genotypes, in accordance with the S-RNase heredity to separate the rule completely in offspring, should be both parents' S-RNase, and that the random 50 seedlings of Eb-2 and Ed-2 are true hybrids.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Cut-style Pollination Can Effectively Overcome Prefertilization Barriers of Distant Hybridization in Loquat

Yang¹,

Fu²

2020

horts

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“…Loquat leaves and flowers are also used to treat cough, asthma, and chronic bronchitis (Kikuchi et al, 2014). Loquat has been cultivated for more than 2 100 years (Yang et al, 2018). In addition to China, more than 30 countries have cultivated loquat, including Spain, Japan, Turkey, India, and Pakistan (Liu et al, 2016;Yang et al, 2018).…”

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Analysis of SSR Information of Flower Transcriptome in Loquat

Fu¹,

Yang²

2023

mpb

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In order to provide scientific basis for studying the genetic diversity and molecular marker-assisted breeding of loquat, new molecular markers of loquat were developed by analyzing flower transcriptome data. In this study, MIcroSAtellite (MISA) and Blast2GO were used for SSR searching, screening, identification, and analysis of non-redundant Unigenes, and Primer 3 was used for SSR primer design. A search found that 28 617 Unigenes contained 44 622 SSR loci, and the frequency of occurrence of SSR loci was 47.51%, and the total average distribution distance was 4.87 kb. Mononucleotide and dinucleotide repeats were the main repeat types of SSR in the loquat flower transcriptome that the SSR of mononucleotide and dinucleotide repeats accounted for 52.09% and 32.83% of the total SSR, respectively. The dominant repeat motifs were A/T and AG/CT, which accounted for 50.94% and 26.70% of the mononucleotide repeats and dinucleotide respectively, and the 65.79% length of motif was concentrated in 12~20 bp. Gene Ontology (GO) functional classification showed that 28 617 loquat flower transcriptome Unigenes containing SSR loci were enriched in 51 GO terms of the 3 GO categories, and the biological process category involved the most GO terms (21). Primers for 34 301 SSR sequences were successfully designed, with a success rate of 76.87%. It is shown by the analysis that the flower transcriptome SSR loci had high frequency, high distribution density, and high polymorphism potential and could provide abundant repeat types. These could provide scientific basis for studying quantitative trait loci mapping, molecular marker-assisted breeding, genome evolution, genetic diversity analysis.

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“…apple [Malus pumila (Li et al, 2011)], plum [Prunus salicina (Nantongo et al, 2016)], loquat [Eriobotrya japonica (Yang et al, 2018)], strawberry [Fragaria ×ananassa (Du et al, 2021)], and pummelo [Citrus maxima (Hu et al, 2021)]. If the S allele of pollen is identical to one of the S alleles in the style, the growth of the pollen tube in the style is inhibited and fertilization fails, which seriously reduces fruit yield (Yang et al, 2015a).…”

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confidence: 99%

Identification of MicroRNAs Related to Phytohormone Signal Transduction and Self-incompatibility of Rabbiteye Blueberry Pollen

Yang

Fu²,

Zhang

et al. 2022

J. Amer. Soc. Hort. Sci.

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MicroRNAs (miRNAs) related to phytohormone signal transduction and self-incompatibility may play an important role in the xenia effect. However, associated research in this area is still lacking in rabbiteye blueberry (Vaccinium ashei). In this study, we identified miRNAs, predicted their target genes, performed functional enrichment analysis of the target genes, and screened for miRNAs related to phytohormone signaling and self-incompatibility. A total of 491 miRNAs were identified, of which 27 and 67 known miRNAs as well as 274 and 416 new miRNAs were found in the rabbiteye blueberry cultivars Brightwell and Premier, respectively. Compared with ‘Premier’, 31 miRNAs were upregulated and 62 miRNAs were downregulated in ‘Brightwell’. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis indicated that the 4985 target genes predicted were involved in biosynthesis of amino acids, plant–pathogen interaction, and spliceosome pathways. A total of 10, one, one, five, two, five, and two candidate miRNAs related to auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinosteroid, and salicylic acid signaling, respectively, in rabbiteye blueberry pollen were identified. Further analysis indicated that novel_miR_49 was a candidate miRNA related to self-incompatibility, and their target gene was maker-VaccDscaff21-snap-gene-21.37. In addition, the KEGG enrichment analysis of the target genes of novel_miR_49 showed that they were involved in the ribosome, aminoacyl-tRNA biosynthesis, and glycosylphosphatidylinositol-anchor biosynthesis pathways. The results revealed that the microRNAs of rabbiteye blueberry pollen regulated to phytohormone signal transduction and self-incompatibility signal transduction based on related to auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinosteroid, and salicylic acid signaling. Results suggest that more research of the effects of miRNAs on regulation of hormone signal transduction and self-incompatibility is necessary for elucidating the molecular mechanism of the xenia effect.

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Identification of 21 novel S-RNase alleles and determination of S-genotypes in 66 loquat (Eriobotrya) accessions

Cited by 5 publications

References 40 publications

Cut-style Pollination Can Effectively Overcome Prefertilization Barriers of Distant Hybridization in Loquat

Cut-style Pollination Can Effectively Overcome Prefertilization Barriers of Distant Hybridization in Loquat

Analysis of SSR Information of Flower Transcriptome in Loquat

Identification of MicroRNAs Related to Phytohormone Signal Transduction and Self-incompatibility of Rabbiteye Blueberry Pollen

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