Identification, functional gastrointestinal stability and molecular docking studies of lentil peptides with dual antioxidant and angiotensin I converting enzyme inhibitory activities

Abstract

The objective was to identify peptides with dual antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities released from lentil proteins by Savinase®. The influence of gastrointestinal digestion on peptide bioactivity was also assayed. Fragments from vicilin, convicilin and legumin were the most abundant peptides identified. Peptides LLSGTQNQPSFLSGF, NSLTLPILRYL, TLEPNSVFLPVLLH showed the highest antioxidant (0.013-1.432μmol Trolox eq./μmol peptide) and ACE inhibitory activities (IC=44-120μM). Gastrointestinal digestion of peptides improved their dual activity (10-14μmol Trolox eq./μmol peptide; IC=11-21μM). In general, C-terminal heptapeptide was crucial for their dual activity. ACE inhibition relies on the formation of hydrogen bonds between C-terminal residues of lentil peptides and residues of the ACE catalytic site. The present study helps clarifying the relationship between structure and dual antioxidant/antihypertensive activity of lentil peptides opening new opportunities to food industry such as the application of lentil protein hydrolysates as ingredients for development of functional foods.

Bioactive Peptides

“…Meanwhile, single peptides with multifunctional bioactivities will be more preferred over single activity peptides as the former would simultaneously elicit multiple health benefits. For this reason, García-Mora et al [170] hydrolyzed lentil proteins using Savinase ® (Novozymes, Bagsvaerd, Denmark) to search for peptides with multiple functions. They observed that peptides LLSGTQNQPSFLSGF, NSLTLPILRYL and TLEPNSVFLPVLLH present in the hydrolysate had strong antioxidant and antihypertensive effects.…”

Synthesis, Biological Evaluation, and Molecular Docking Studies of Some N‐thiazolyl Hydrazones and Indenopyrazolones

“…The synthesized indenopyrazolones ( 4a – h ) were screened for their in vitro antioxidant activity by using the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging assay . Ascorbic acid was used as a standard reference.…”

Food Proteins and Bioactive Peptides

“…Sheep have also been demonstrated to benefit from microalgae as a protein source, with lambs reported to have increased average daily gains upon consumption of 10 g of Spirulina per day [170]. Similarly, Spirulina diet supplementation increased the feed intake of rabbits, as well as improve the quality of rabbit meat with higher levels of GLA [171].…”