Identification and study of tobacco mosaic virus movement function by complementation tests

Atabekov, J.G.; Malyshenko, S. I.; Morozov, Sergey Y.; Taliansky, M. E.; Solovyev, Andrey G.; Agranovsky, A. A.; Shapka, Natalia

doi:10.1098/rstb.1999.0414

Cited by 33 publications

(21 citation statements)

References 48 publications

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“…However, several lines of evidence support a role for other viral proteins in the movement of tobamoviruses. Complementation studies of movement between related and unrelated viruses suggests a need for ongoing replication or synthesis of replication proteins (Atabekov et al. , 1999; Deom et al.…”

Section: Replication Proteinsmentioning

confidence: 99%

Tobacco mosaic virus, not just a single component virus anymore

Knapp

Lewandowski

2001

Molecular Plant Pathology

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Summary Taxonomy: Tobacco mosaic virus (TMV) is the type species of the Tobamovirus genus and a member of the alphavirus-like supergroup. Historically, many tobamoviruses are incorrectly called strains of TMV, although they can differ considerably in sequence similarities and host range from each other and from TMV. Physical properties: TMV virions are 300 x 18 nm rods with a central hollow cavity (Fig. 1) and are composed of 95% capsid protein (CP), and 5% RNA. Each CP subunit interacts with 3-nts in a helical arrangement around the RNA. Virions are stable for decades; infectivity in sap survives heating to 90 degrees C. Hosts: The natural host range of TMV is limited; however, a broad range of weed and crop species, mostly Solanaceae that includes tobacco, pepper and tomato can be infected experimentally [Holmes, F.O. (1946) A comparison of the experimental host ranges of tobacco etch and tobacco mosaic viruses. Phytopathology, 36, 643-657]. TMV distribution is worldwide. No biological vectors are known. Useful website: http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/71010001.htm.

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Section: Replication Proteinsmentioning

confidence: 99%

Tobacco mosaic virus, not just a single component virus anymore

Knapp

Lewandowski

2001

Molecular Plant Pathology

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“…13 Many taxonomically-distinct viruses encode movement proteins that are interchangeable for viral RNA transport. 14 For instance, a hybrid Cowpea chlorotic mottle virus, constructed by replacing its 3a movement protein (MP) with the 30K MP of Sunn-hemp mosaic virus was able to establish a systemic infection of cowpea. 15 Tobamovirus and dianthovirus MPs are functionally homologous.…”

Section: Introductionmentioning

confidence: 99%

Suppression of local RNA silencing is not sufficient to promote cell-to-cell movement ofTurnip crinkle virusinNicotiana benthamiana

Shi

Ryabov

Wezel

et al. 2009

Plant Signaling & Behavior

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The biological relationship between suppression of RNA silencing and virus movement poses an intriguing question in virus-plant interactions. Here, we have used a local RNA silencing assay, based on a movement-deficient Turnip crinkle virus TCV/ GFPΔCP, to investigate the influence of silencing suppression by three different viral suppressors: the TCV 38K coat protein (CP), the 126K protein of Tobacco mosaic virus (TMV), and P19 of Tomato bushy stunt virus (TBSV) on cell-to-cell movement and long-distance spread of TCV/GFPΔCP. First, we found that TCV CP blocked the induction of local RNA silencing, but failed to support virus trafficking in silencing-suppressed transgenic plants, although it acted as a functional movement protein in nontransformed plants. Second, we demonstrated that the TMV 126K suppressor inhibited TCV/GFPΔCP-mediated RNA silencing, but did not facilitate intercellular spread of the chimaeric carmovirus. However, TMV and TMVΔCP prevented the initiation of RNA silencing by TCV/GFPΔCP and caused TCV/GFPΔCP to move between cells, although only TMV supported its long-distance spread. Third, TBSV P19 functioned as a movement protein for TCV/GFPΔCP and as a silencing suppressor in non-transformed and silencing-suppressed transgenic plants. We further identified three types of mutant P19 proteins that possessed no or varied functionality in silencing suppression and in the facilitation of carmovirus movement. These results suggest that, although suppression of local RNA silencing is essential for the maintenance of viral RNA, recovery of cell-to-cell movement and long-distance spread of movement-deficient carmoviruses is not a direct consequence of such silencing suppression.

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“…Trans-complementation is a common phenomenon among animal and plant viruses (Atabekov et al, 1999;Garcia-Arenal et al, 2001;Heldsinger & Sharmeen, 2001;Lough et al, 2001;Osbourn et al, 1990;Saphire et al, 2002;van Kuppeveld et al, 2002). A full-length replicationdefective mutant of the coxsackie B3 virus 2B protein can be complemented by its wild-type virus (van Kuppeveld et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Mutation of Phe50 to Ser50 in the 126/183-kDa proteins of Odontoglossum ringspot virus abolishes virus replication but can be complemented and restored by exact reversion

Wang

Wong

2004

Journal of General Virology

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Sequence comparison of a non-biologically active full-length cDNA clone of Odontoglossum ringspot virus (ORSV) pOT1 with a biologically active ORSV cDNA clone pOT2 revealed a single nucleotide change of TRC at position 211. This resulted in the change of Phe50 in OT2 to Ser50 in OT1. It was not the nucleotide but the amino acid change of Phe50 that was responsible for the inability of OT1 to replicate. Time-course experiments showed that no minus-strand RNA synthesis was detected in mutants with a Phe50 substitution. Corresponding mutants in Tobacco mosaic virus (TMV) showed identical results, suggesting that Phe50 may play an important role in replication in all tobamoviruses. Complementation of a full-length mutant OT1 was demonstrated in a co-infected local-lesion host, a systemic host and protoplasts by replication-competent mutants tORSV.GFP or tORSV.GFPm, and further confirmed by co-inoculation using tOT1.GFP+tORSV (TTC), suggesting that ORSV contains no RNA sequence inhibitory to replication in trans. Surprisingly, a small number of exact revertants were detected in plants inoculated with tOT1+tORSV.GFPm or tOT1.GFP+tORSV (TTC). No recombination was detected after screening of silent markers in virus progeny extracted from total RNA or viral RNA from inoculated and upper non-inoculated leaves as well as from transfected protoplasts. Exact reversion from TCT (OT1) to TTT (OT2), rather than recombination, restored its replication function in co-inoculated leaves of Nicotiana benthamiana.

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Identification and study of tobacco mosaic virus movement function by complementation tests

Cited by 33 publications

References 48 publications

Tobacco mosaic virus, not just a single component virus anymore

Tobacco mosaic virus, not just a single component virus anymore

Suppression of local RNA silencing is not sufficient to promote cell-to-cell movement ofTurnip crinkle virusinNicotiana benthamiana

Mutation of Phe50 to Ser50 in the 126/183-kDa proteins of Odontoglossum ringspot virus abolishes virus replication but can be complemented and restored by exact reversion

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