Identification and Regulation of Genes from a Biocontrol Strain of <i>Fusarium oxysporum</i>

Fravel, D. R.; Moravec, Brian C; Bailey, Bryan A.

doi:10.1111/j.1439-0434.2007.01271.x

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Knowledge of the modes of action may provide avenues to expand the use of this biocontrol agent. Previous research that was done to clarify mechanisms underlying the plant-mediated biocontrol effect of CS-20 was focused on identification and characterization of genes related to its biocontrol ability to identify differences in gene regulation between CS-20 and pathogenic FOL isolates ( Fravel et al, 2003a , 2007 , 2008 ). The work reported here was undertaken to further clarify the plant-mediated mode of action of CS-20 by determining its ability to produce elicitors of defense responses, which are associated with local and/or systemic resistance and result in reduction of wilt severity on tomatoes.…”

Section: Introductionmentioning

confidence: 99%

Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato

et al. 2016

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The biocontrol effect of the non-pathogenic Fusarium oxysporum strain CS-20 against the tomato wilt pathogen F. oxysporum f. sp. lycopersici (FOL) has been previously reported to be primarily plant-mediated. This study shows that CS-20 produces proteins, which elicit defense responses in tomato plants. Three protein-containing fractions were isolated from CS-20 biomass using size exclusion chromatography. Exposure of seedling roots to one of these fractions prior to inoculation with pathogenic FOL strains significantly reduced wilt severity. This fraction initiated an ion exchange response in cultured tomato cells resulting in a reversible alteration of extracellular pH; increased tomato chitinase activity, and induced systemic resistance by enhancing PR-1 expression in tomato leaves. Two other protein fractions were inactive in seedling protection. The main polypeptide (designated CS20EP), which was specifically present in the defense-inducing fraction and was not detected in inactive protein fractions, was identified. The nucleotide sequence encoding this protein was determined, and its complete amino acid sequence was deduced from direct Edman degradation (25 N-terminal amino acid residues) and DNA sequencing. The CS20EP was found to be a small basic cysteine-rich protein with a pI of 9.87 and 23.43% of hydrophobic amino acid residues. BLAST search in the NCBI database showed that the protein is new; however, it displays 48% sequence similarity with a hypothetical protein FGSG_10784 from F. graminearum strain PH-1. The contribution of CS20EP to elicitation of tomato defense responses resulting in wilt mitigating is discussed.

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Section: Introductionmentioning

confidence: 99%

Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato

et al. 2016

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“…Interestingly, our proteome analysis identified many proteins involved in nutrient uptake of both fungal and bacterial origin (Tables 1 and 2), e.g . ABC transporters, solute‐binding proteins, amino acid‐, oligopeptide‐, and sugar‐transporters which have been implicated in biocontrol 29–31.…”

Section: Resultsmentioning

confidence: 99%

A proteomics approach to study synergistic and antagonistic interactions of the fungal–bacterial consortiumFusarium oxysporumwild‐type MSA 35

et al. 2010

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Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multispecies consortium that consists of a fungus and numerous rhizobacteria mainly belonging to gamma-proteobacteria, was analyzed by two complementary metaproteomic approaches (2-DE combined with MALDI-Tof/Tof MS and 1-D PAGE combined with LC-ESI-MS/MS) to identify fungal or bacterial factors potentially involved in antagonistic or synergistic interactions between the consortium members. Moreover, the proteome profiles of F. oxysporum WT MSA 35 and its cured counter-part CU MSA 35 (WT treated with antibiotics) were compared with unravel the bacterial impact on consortium functioning. Our study presents the first proteome mapping of an antagonistic F. oxysporum strain and proposes candidate proteins that might play an important role for the biocontrol activity and the close interrelationship between the fungus and its bacterial partners.

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Detection and Identification of Fungal Biological Control Agents

Narayanasamy¹

2013

Biological Management of Diseases of Crops

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Identification and Regulation of Genes from a Biocontrol Strain of Fusarium oxysporum

Cited by 4 publications

References 18 publications

Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato

Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato

A proteomics approach to study synergistic and antagonistic interactions of the fungal–bacterial consortiumFusarium oxysporumwild‐type MSA 35

Detection and Identification of Fungal Biological Control Agents

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