Identification and purification of novel chlorogenic acids in Artemisia annua L.

Abstract

Present work has been carried out to study the identification and purification of chlorogenic acids in Artemisia annua L. Thirty-six chlorogenic acids were identified from this plant. Among these fifteen viz. two monocaffeoylquinic acids (Mr354), five dicaffeoylquinic acids (Mr516), one feruloylquinic acid (Mr368), three caffeoylferuloylquinic acids (Mr530), two ferulylquinic acids (Mr544), one dimethoxy-cinnamoylquinic acid (Mr382) and one p-coumaroylquinic acid (Mr338) were reported first time in present study by LC/MS n . Cis-isomers of these chlorogenic acids were also identified.Furthermore, column chromatography was used for the separation and purification of these chlorogenic acid; by the use of petroleum ether and ethyl acetate decolorization methods as mentioned in the literature, thus separation and purification process carried out at the same time. Polyamide and dextran were also used to purify Dicaffeoylquinic acid and purity level reached 85.7% with a yield of 53.4% after the secondary purification by Sephadex LH-20. Result of study revealed that A. annua can not only used for the production of artemisinin, but also yielding different kinds of chlorogenic acids, thus making comprehensive utilization of this plant.