Identification and molecular characterization of a novel varicosa-like virus from red clover

Koloniuk, Igor; Fráňová, Jana; Sarkisová, Tatiana; Přibylová, Jaroslava; Lenz, Ondřej; Petrzik, Karel; Špak, Josef

doi:10.1007/s00705-018-3838-2

Cited by 20 publications

(34 citation statements)

References 20 publications

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“…A considerable disadvantage of growing clover is its limited persistence, due in large measure to its susceptibility to viral and phytoplasma diseases (Edwardson & Christie, ; Taylor & Quesenberry, ; Fránová et al , ; Jones, ). More than 30 viruses are known to infect red clover (Edwardson & Christie, ), and this list could certainly be extended with further discoveries (Fránová et al , ; Fránová & Jakešová, ; Koloniuk & Fránová, ; Lenz et al , ; Koloniuk et al , ,).…”

Section: Introductionmentioning

confidence: 99%

Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Fráňová

Sarkisová

Jakešová³

et al. 2019

Plant Pathology

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Three pairs of generic primers designed for specific reverse transcription polymerase chain reaction amplification of part of the cytorhabdoviruses genomes were successfully tested with a number of red clover samples. Two infected plants showing irregular vein clearing, tissue necrosis and stunted growth symptoms were selected for further studies. Subsequently, two novel plant rhabdoviruses, tentatively named Trifolium pratense virus A (TpVA) and Trifolium pratense virus B (TpVB), were detected in red clover plants. Sequence analyses of nucleotide sequences of their genomic RNAs obtained after high‐throughput sequencing confirmed their affinity to members of the genus Cytorhabdovirus. While TpVA was transmitted by mechanical inoculation to Nicotiana occidentalis 37B and subtransferred to Physalis floridana plants, crude sap transmission of TpVB to a range of herbaceous host plants failed. The bacilliform shape of virions, which has a cytoplasm‐limited distribution, their sizes, and the phylogenetic relationships, support assignment of TpVA and TpVB to two distinct species of the genus Cytorhabdovirus.

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Section: Introductionmentioning

confidence: 99%

Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Fráňová

Sarkisová

Jakešová³

et al. 2019

Plant Pathology

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“…Missing sequence segments were obtained by PCR amplification using the Q5 High-Fidelity Master Mix (NEB, Ipswich, MA, USA). The 5′-termini were completed and sequenced with a 5′ rapid amplification of complementary DNA (cDNA) ends (RACE) kit (Invitrogen, Carlsbad, CA, USA), and the 3′-ends were derived as previously described [ 17 ]. Sequence verification and gap-filling were done through Sanger sequencing of PCR amplicons or cloned into a pGEM T-Easy vector system (Promega, Road Madison, WI, USA).…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species

Koloniuk

Thekke-Veetil

Reynard

et al. 2018

Viruses

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Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

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“…As shown in Figure 6, there were nine major components (1)(2)(3)(4)(5)(6)(7)(8)(9) in the T. pratense L. extract. After the use of methanol to denature the enzyme and dislodge the specifically bound compounds and the ultrasonication of the extract with COX-2-coated magnetic nanoparticles, two main peaks, 4 and 6, completely disappeared in the supernatant, while the peak area of 8 and 9 were significantly reduced.…”

Section: Analysis Of Binding Compounds From Trifolium Pratense L Extmentioning

confidence: 99%

Screening and isolation of cyclooxygenase‐2 inhibitors from Trifolium pratense L. via ultrafiltration, enzyme‐immobilized magnetic beads, semi‐preparative high‐performance liquid chromatography and high‐speed counter‐current chromatography

Hou

et al. 2019

J of Separation Science

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Nonsteroidal anti‐inflammatory drugs reportedly reduce the risk of developing cancer. One mechanism by which they reduce carcinogenesis involves the inhibition of the activity of cyclooxygenase‐2, an enzyme that is overexpressed in various cancer tissues. Its overexpression increases cell proliferation and inhibits apoptosis. However, selected cyclooxygenase‐2 inhibitors can also act through cyclooxygenase‐independent mechanisms. In this study, using ultrafiltration, enzyme‐immobilized magnetic beads, high‐performance liquid chromatography, and electrospray‐ionization mass spectrometry, several isoflavonoids in Trifolium pratense L. extracts were screened and identified. Semi‐preparative high‐performance liquid chromatography and high‐speed counter‐current chromatography were then applied to separate the active constituents. Using these methods, seven major compounds were identified in Trifolium pratense L. As cyclooxygenase‐2 inhibitors: rothindin, ononin, daidzein, trifoside, pseudobaptigenin, formononetin, and biochanin A, which were then isolated with >92% purity. This is the first report of the presence of potent cyclooxygenase‐2 inhibitors in Trifolium pratense L. extracts. The results of this study demonstrate that the systematic isolation of bioactive components from Trifolium pratense L., by using ultrafiltration, enzyme‐immobilized magnetic beads, semi‐preparative high‐performance liquid chromatography, and high‐speed counter‐current chromatography, represents a feasible and efficient technique that could be extended for the identification and isolation of other enzyme inhibitors.

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Identification and molecular characterization of a novel varicosa-like virus from red clover

Cited by 20 publications

References 20 publications

Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species

Screening and isolation of cyclooxygenase‐2 inhibitors from Trifolium pratense L. via ultrafiltration, enzyme‐immobilized magnetic beads, semi‐preparative high‐performance liquid chromatography and high‐speed counter‐current chromatography

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