Identification and Molecular Characterization of a Novel Partitivirus from Trichoderma atroviride NFCF394

Chun, Jeesun; Yang, Han-Eul; Kim, Dae-Hyuk

doi:10.3390/v10110578

Cited by 18 publications

(31 citation statements)

References 22 publications

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“…In addition, dsRNAs have also been detected in some populations of Trichoderma spp., as a causing agent of green mold disease in mushrooms in Korea [44]. Recently, more mycoviruses have been detected in T. atroviride [45,46], T. harzianum [46], and T. asperellum [47]. Within these reports, one research showed that infection by Trichoderma harzianum partitivirus 1 (ThPV1) may increase the antifungal activity of T. harzianum [46].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, more mycoviruses have been detected in T. atroviride [45,46], T. harzianum [46], and T. asperellum [47]. Within these reports, one research showed that infection by Trichoderma harzianum partitivirus 1 (ThPV1) may increase the antifungal activity of T. harzianum [46]. However, the effects of ThPV1 infection on the biocontrol efficacy of T. harzianum remains unknown.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

et al. 2019

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Trichoderma species are a group of fungi which is widely distributed in major terrestrial ecosystems; they are also commonly used as biocontrol agents for many plant diseases. A virus, namely Trichoderma harzianum hypovirus 1 (ThHV1), was identified in T. harzianum isolate T-70, and also infected isolate T-70D, together with its defective RNA (ThHV1-S). The ThHV1 genome possessed two Open Reading Frames (ORFs), namely ORF1 and ORF2. The start codon of ORF2 overlapped with the stop codon of ORF1 in a 43 nt long region. The polypeptide encoded by ORF2 of ThHV1 shared sequence similarities with those of betahypoviruses, indicating that ThHV1 is a novel member of Hypoviridea. Isolate T-70D, carrying both ThHV1 and ThHV1-S, showed abnormal biological properties, notably a decreased mycoparasitism ability when compared with isolate T-70. Both ThHV1 and ThHV1-S could be vertically transmitted to conidia and horizontally transmitted to T. harzianum isolate T-68 and T. koningiopsis T-51. The derivative strains carrying both ThHV1 and ThHV1-S showed decreased mycoparasitism ability, whereas strains carrying ThHV1 alone were normal, indicating that ThHV1-S is closely associated with the decreased mycoparasitism ability of T. harzianum isolate T-70D. ThHV1 was widely detected in isolates of T. harzianum, T. koningiopsis and T. atroviride originating from soil of China. Therefore, viruses in fungal biocontrol agents may also be a factor associated with the stability of their application.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

et al. 2019

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“…Moreover, an unclassified dsRNA mycovirus isolated from T. atroviride NFCF028 was annotated as Trichoderma atroviride mycovirus 1 (TaMV1) [ 15 ]. Complete genome sequencing of dsRNA viruses from T. atroviride NFCF394 and T. harzianum NFCF319 identified Trichoderma atroviride partitivirus 1 (TaPV1) and Trichoderma harzianum partitivirus 1 (ThPV1) as novel mycoviruses of the family of Partitiviridae [ 16 , 17 ]. More recently, five novel mycoviruses, i.e., Trichoderma asperellum dsRNA virus 1 (TaRV1) [ 18 ], Trichoderma harzianum mycovirus 1 (ThMBV1) [ 19 ], Trichoderma harzianum mycovirus 1 (ThMV1) [ 20 ], Trichoderma harzianum hypovirus 1 (ThHV1) [ 21 ], and the new (+)ss RNA hypovirus Trichoderma harzianum hypovirus 2 (ThHV2) [ 22 ], were identified.…”

Section: Introductionmentioning

confidence: 99%

The Newly Identified Trichoderma harzianum Partitivirus (ThPV2) Does Not Diminish Spore Production and Biocontrol Activity of Its Host

Wang

Liu

Jiang

et al. 2022

Viruses

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A new partititvirus isolated from a Trichoderma harzianum strain (T673), collected in China, was characterized and annotated as Trichoderma harzianum partitivirus 2 (ThPV2). The genome of ThPV2 consists of a 1693 bp dsRNA1 encoding a putative RNA-dependent RNA polymerase (RdRp) and a 1458 bp dsRNA2 encoding a hypothetical protein. In comparative studies employing the ThPV2-infected strain (T673) and a strain cured by ribavirin treatment (virus-free strain T673-F), we investigated biological effects of ThPV2 infection. While the growth rate of the virus-infected fungus differed little from that of the cured variant, higher mycelial density, conidiospore, and chlamydospore production were observed in the virus-infected strain T673. Furthermore, both the ThPV2-infected and the cured strain showed growth- and development-promoting activities in cucumber plants. In vitro confrontation tests showed that strains T673 and T673-F inhibited several important fungal pathogens and an oomycete pathogen in a comparable manner. Interestingly, in experiments with cucumber seeds inoculated with Fusarium oxysporum f. sp. cucumerinum, the ThPV2-infected strain T673 showed moderately but statistically significantly improved biocontrol activity when compared with strain T673-F. Our data broaden the spectrum of known mycoviruses and provide relevant information for the development of mycoviruses for agronomic applications.

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“…Pittosporum cryptic virus 1 was identified from an Italian pittosporum plant by NGS with extracted dsRNA [6]. Similarly, application of NGS to extracted dsRNA has resulted in identification of Trichoderma atroviride partitivirus 1 from the fungal species Trichoderma atroviride [7].…”

Section: Introductionmentioning

confidence: 99%

Identification of viruses belonging to the familyPartitiviridaefrom plant transcriptomes

Cho

2020

Preprint

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1Viruses in the family Partitiviridae consist of non-enveloped viruses with bisegmented double-2 stranded RNA genomes. Viruses in this family have been identified from plants and fungi. In 3 this study, we identified several viruses belonging to the family Partitiviridae using plant 4 transcriptomes. From 11 different plant species, we identified a total of 74 RNA segments 5 representing 23 partitiviruses. Of 74 RNA segments, 28 RNA segments encode RNA-6 dependent RNA polymerases (RdRp) while 46 RNA segments encode coat proteins (CPs). 7According to ICTV demarcation for the family Partitiviridae, 25 RNAs encoding RdRp and 8 41 RNAs encoding CP were novel RNA segments. In addition, we identified eight RNA 9 segments (three for RdRp and five for CP) belonging to the known partitivruses. Taken together, 10 this study provides the largest number of partitiviruses from plant transcriptomes in a single 11 study. Viruses in the family Partitiviridae consist of non-enveloped viruses with bisegmented double-2 stranded (ds) RNA genomes [1]. Each RNA segment of the viruses in the family Partitiviridae 3 encodes a single protein including RNA-dependent RNA polymerase (RdRp) or coat protein 4 (CP). Members in the family Partitiviridae can be divided into five different genera according 5 to the host. For example, viruses in the genera Alphapartitivirus and Betapartitivirus are 6 identified from either plants or fungi while viruses in the genus Gammapartitivirus and 7 Deltapartitivirus are derived from only fungi and plants, respectively [1]. In addition, viruses 8 in the genus Cryspovirus are identified from protozoa. Transmission of partitiviruses is 9 occurred by seeds (plants), cell division and sporogenesis (fungi), and oocytes (protozoa) [1]. 10 To date, there are more than 45 partitivirus species. In particular, there are five deltapartitivirus 11 species infecting plants including Pepper cryptic virus 1 (PCV-1) [2], Pepper cryptic virus 2 12 (PCV-2), Fig cryptic virus (FCV) [3], Beet cryptic virus 2 (BCV2), and Beet cryptic virus 3 13 (BCV3) [4] according to International Committee on Taxonomy of Viruses (ICTV). 14 To identify novel partitiviruses with dsRNA genomes, dsRNA extraction followed by 15 next-generation sequencing (NGS) is an efficient approach. Based on those approaches, several 16 partitiviruses have been identified. For example, Melon necrotic spot virus was identified from 17 watermelon plant (Citrullus lanatus Thunb) using SOLiD NGS analysis [5]. Pittosporum 18 cryptic virus 1 was identified from an Italian pittosporum plant by NGS with extracted dsRNA 19 [6]. Similarly, application of NGS to extracted dsRNA has resulted in identification of 20 Trichoderma atroviride partitivirus 1 from the fungal species Trichoderma atroviride [7]. 21 Moreover, instead of traditional molecular cloning methods, in silico data analyses has 22 resulted in identification of diverse dsRNA viruses from expressed sequence tag (EST) 23 database [8] and plant transcriptomes [9-13]. Moreover, 120 mycoviruses infecting fungi have...

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Identification and Molecular Characterization of a Novel Partitivirus from Trichoderma atroviride NFCF394

Cited by 18 publications

References 22 publications

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

The Newly Identified Trichoderma harzianum Partitivirus (ThPV2) Does Not Diminish Spore Production and Biocontrol Activity of Its Host

Identification of viruses belonging to the familyPartitiviridaefrom plant transcriptomes

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