Identification and mapping of quantitative resistance to late blight (Phytophthora infestans) in Solanum habrochaites LA1777

Li, Junming; Liu, Lei; Bai, Yuling; Finkers, Richard; Wang, Feng; Du, Yan; Yang, Youyou; Xie, Bingyan; Visser, R.G.F.; Heusden, A.W. van

doi:10.1007/s10681-010-0340-7

Cited by 46 publications

(47 citation statements)

References 35 publications

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“…We focused on identifying QTL from an existing IL population derived from S. habrochaites LA1777 to identify specific ILs that could be ultimately deployed more rapidly into a breeding program. QTLs conferring resistance to Botrytis cinerea and Phytophthora infestans have been detected in the LA1777 × E6203 IL population (Gabor et al 2010;Li et al 2011). In this study, four QTLs conferring resistance to Pst A9 were detected above the permutation threshold, located on chromosomes 1, 2 and 12 (Table 2; Fig.…”

Section: Germplasm Screeningsupporting

confidence: 48%

“…At least five introgression line (IL) populations have been generated following a cross to a wild tomato species, including ILs derived from: S. pennellii LA716 (Eshed and Zamir 1994), S. habrochaites LA1777 (Monforte and Tanksley 2000), S. habrochaites LA407 (Francis et al 2001), S. habrochaites LYC4 (Finkers et al 2007) and S. lycopersicoides LA2951 (Canady et al 2005). Populations of ILs that contain only a fragment of the wild donor genome are useful for the discovery of disease resistance QTLs (Li et al 2011;Smart et al 2007). These ILs have been used to identify QTLs affecting yield as well as other desirable attributes such as carotenoid production and accumulation of primary metabolites (Fridman et al 2004;Schauer et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…S. pennellii LA716-derived ILs have been extensively phenotyped and genotyped to identify QTLs controlling various diseases (Astua-Monge et al 2005), fruit quality (Rousseaux et al 2005;Smart et al 2007) and yield . Recently, resistance against late blight caused by the oomycete pathogen Phytophthora infestans and gray mold caused by fungus Botrytis cinerea has been detected in LA1777 ILs (Gabor et al 2010;Li et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Identification of QTLs controlling resistance to Pseudomonas syringae pv. tomato race 1 strains from the wild tomato, Solanum habrochaites LA1777

Thapa

Miyao²,

Davis

et al. 2015

Theor Appl Genet

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Screening of wild tomato accessions revealed a source of resistance to Pseudomonas syringe pv. tomato race 1 from Solanum habrochaites and facilitated mapping of QTLs controlling disease resistance. Pseudomonas syringae pv. tomato (Pst) causes bacterial speck of tomato, which is one of the most persistent bacterial diseases in tomato worldwide. Existing Pst populations have overcome genetic resistance mediated by the tomato genes Pto and Prf. The objective of this study was to identify sources of resistance to race 1 strains and map quantitative trait loci (QTLs) controlling resistance in the wild tomato Solanum habrochaites LA1777. Pst strains A9 and 407 are closely related to current field strains and genome sequencing revealed the lack of the avrPto effector as well as select mutations in the avrPtoB effector, which are recognized by Pto and Prf. Strains A9 and 407 were used to screen 278 tomato accessions, identifying five exhibiting resistance: S. peruvianum LA3799, S. peruvianum var. dentatum PI128655, S. chilense LA2765, S. habrochaites LA2869, and S. habrochaites LA1777. An existing set of 93 introgression lines developed from S. habrochaites LA1777 was screened for resistance to strain A9 in a replicated greenhouse trial. Four QTLs were identified using composite interval mapping and mapped to different chromosomes. bsRr1-1 was located on chromosome 1, bsRr1-2 on chromosome 2, and bsRr1-12a and bsRr1-12b on chromosome 12. The QTLs detected explained 10.5-12.5% of the phenotypic variation. Promising lines were also subjected to bacterial growth curves to verify resistance and were analyzed for general horticultural attributes under greenhouse conditions. These findings will provide useful information for future high-resolution mapping of each QTL and integration into marker-assisted breeding programs.

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Section: Germplasm Screeningsupporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of QTLs controlling resistance to Pseudomonas syringae pv. tomato race 1 strains from the wild tomato, Solanum habrochaites LA1777

Thapa

Miyao²,

Davis

et al. 2015

Theor Appl Genet

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“…Therefore, the gene-based SCAR marker can be considered as a functional marker for the Ph3 resistance locus. In addition to these single dominant resistance genes, quantitative trait loci (QTL) that confer resistance to LB have also been identified in S. habrochaites and S. penellii (Brouwer and St Clair 2004;Smart et al 2007;Li et al 2011;Cai et al 2012).…”

Section: Late Blight Resistancementioning

confidence: 99%

Molecular Markers for Selecting Diverse Disease Resistances in Tomato Breeding Programs

Lee¹,

Oh²,

Yeam³

2015

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Tomato (Solanum lycopersicum L.) is an economically important crop worldwide. In addition, tomato serves as an excellent model system for plant genetics and biology, including fruit biology, abiotic stress tolerance, and plant-microbe interactions. Development and practical use of molecular markers have been actively pursued in molecular breeding programs for tomato, especially for disease resistance to allow selection of a single resistance gene and combination of multiple resistance genes. Due to insufficient genetic variation in cultivated tomatoes, various wild relatives of tomato have been investigated and utilized as disease resistance sources. In order to pursue the resistance provided by these wild relatives in developing new tomato varieties, molecular markers have been developed and intensively utilized in tomato breeding programs. In this review, we summarize the currently available molecular markers that confer resistance against major tomato diseases, including Tomato yellow leaf curl virus (TYLCV), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV), verticillium wilt, fusarium wilt, late blight caused by Phytophthora infestans, leaf mold caused by Cladosporium fulvum, root-knot caused by Meloidogyne spp., bacterial spot caused by Xanthomonas spp., and bacterial speck caused by Pseudomonas syringae. The provided marker information is expected to contribute to development of marker-assisted selection for disease resistance and to exploration of novel genetic sources for a tomato breeding program.

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“…Occasionally, all R genes in one cluster are functional, as is the case for R3 locus for late blight resistance in potato (Huang et al 2005; Li et al 2011b). In this study, however, only one RGA in the Ph - 3 cluster contributes to the resistance for late blight.…”

Section: A Hotspot Carrying Rpi Genes On Chromosome 9 Of Solanum Speciesmentioning

confidence: 99%

The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans

et al. 2014

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Key messagePh-3is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein.AbstractLate blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-014-2303-1) contains supplementary material, which is available to authorized users.

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Identification and mapping of quantitative resistance to late blight (Phytophthora infestans) in Solanum habrochaites LA1777

Cited by 46 publications

References 35 publications

Identification of QTLs controlling resistance to Pseudomonas syringae pv. tomato race 1 strains from the wild tomato, Solanum habrochaites LA1777

Identification of QTLs controlling resistance to Pseudomonas syringae pv. tomato race 1 strains from the wild tomato, Solanum habrochaites LA1777

Molecular Markers for Selecting Diverse Disease Resistances in Tomato Breeding Programs

The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans

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