Identification and Localization of Proteins Associated with Biomineralization in the Iron Deposition Vesicles of Honeybees (Apis mellifera)

2015

Apidologie

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-The lifespans of queen honeybees (Apis mellifera ) are much longer than those of worker bees. Mitochondrial energy utilization in the trophocytes and oenocytes of worker bees during aging has been determined, but it remains unknown in queen bees. In this study, mitochondrial energy utilization was assayed in the trophocytes and oenocytes of young and old queen bees. The mitochondrial density and mitochondrial membrane potential (Δψm); nicotinamide adenine dinucleotide (NAD + ), nicotinamide adenine dinucleotide reduced form (NADH), and adenosine triphosphate (ATP) levels; NAD + /NADH ratio; and relative expression of NADH dehydrogenase 1 (ND1) and ATP synthase normalized against mitochondrial density were not significantly different between young and old queen bees. These results indicate that mitochondrial energy utilization maintains a young status in the trophocytes and oenocytes of old queen bees and that trophocytes and oenocytes have longevity-promoting mechanisms that can be investigated to clarify the secret of longevity in queen bees.

Section: Assay Of Mitochondrial Densitymentioning

confidence: 99%

Mitochondrial energy utilization maintains young status in the trophocytes and oenocytes of old queen honeybees

2015

Apidologie

Self Cite

“…Thin sections (60-90 nm in thickness) were cut with a diamond knife, stained with uranyl acetate and lead citrate, and then examined using a TEM system (JEOL JEM-2000EXII; Tokyo, Japan) operating at an accelerating voltage of 100 kV (Hsu and Chan 2011b). This experiment was biologically replicated ten times and used a total of ten young and ten old workers.…”

Section: Assay Of Mitochondrial Densitymentioning

confidence: 99%

Changes in mitochondrial energy utilization in young and old worker honeybees (Apis mellifera)

Chuang

2012

AGE

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Trophocytes and fat cells in honeybees (Apis mellifera) have served as targets for cellular senescence studies, but mitochondrial energy utilization with advancing age in workers is unknown. In this study, mitochondrial energy utilization was evaluated in the trophocytes and fat cells of young and old workers reared in a field hive. The results showed that (1) mitochondrial density increased with advancing age; (2) mitochondrial membrane potential (ΔΨm), nicotinamide adenine dinucleotide oxidized form (NAD + ) concentration, adenosine triphosphate (ATP) concentration, and NAD + /nicotinamide adenine dinucleotide reduced form (NADH) ratio decreased with advancing age; and (3) the expression of NADH dehydrogenase 1 (ND1), ATP synthase, and voltage-dependent anion channel 1 (VDAC1) increased with advancing age, whereas ND1 and ATP synthase did not differ with advancing age after normalization to mitochondrial density and VDAC1. These results show that the trophocytes and fat cells of young workers have higher mitochondrial energy utilization efficiency than those of old workers and that aging results in a decline in mitochondrial energy utilization in the trophocytes and fat cells of worker honeybees.

“…When IGs are mature, they are filled to the inner membrane, leaving a 10 nm space between IGs and the outer membrane of IDVs 11 . An actin-myosin-ferritin transporter system participates in the formation of IGs 12 . Actin, myosin, ferritin 2, and ATP synthase are involved in this system 12 .…”

Section: Introductionmentioning

confidence: 99%

“…An actin-myosin-ferritin transporter system participates in the formation of IGs 12 . Actin, myosin, ferritin 2, and ATP synthase are involved in this system 12 .…”

Section: Introductionmentioning

confidence: 99%

Ferritin RNA interference inhibits the formation of iron granules in the trophocytes of worker honey bees (Apis mellifera)

Mutti

Amdam

2019

Sci Rep

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Iron granules containing superparamagnetic magnetite act as magnetoreceptor for magnetoreception in honey bees. Biomineralization of iron granules occurs in the iron deposition vesicles of trophocytes and requires the participation of actin, myosin, ferritin2, and ATP synthase. The mechanism of magnetoreception in honey bees can be explored by suppressing the formation of iron granules. Toward this goal, we injected double-stranded RNA of ferritin2 and ferritin1 into newly emerged worker honey bees to knock down these genes via RNA interference. We confirmed that mRNA and protein production of the ferritins was inhibited, leading to immature iron granules. Downregulating ferritin2 and ferritin1 , moreover, leads to different deposition morphology of 7.5-nm diameter iron particles, indicating that the two genes play different roles in the formation of iron granules in worker honey bees.