Identification and in vitro antioxidant activities of phenolic compounds isolated from Cynoglossum cheirifolium L.

Abstract

In an extensive search for bioactive compounds from plant sources, the quantitative and qualitative characterisation of the compounds present in Cynoglossum cheirifolium extracts was studied. Total phenolic and flavonoid contents were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined through DPPH scavenging activity and Ferric reducing antioxidant power (FRAP). Our study showed that leaves produce more phenolic compounds, followed by flowering aerial part. The butanolic fraction obtained from leaves extract exhibited the highest total phenolics (78.65 ± 3.58 mg GAE/g DW) and flavonoids (22.15 ± 4.66 mg CE/g DW). In contrast, this fraction displayed the highest DPPH scavenging ability with IC values of 0.06 ± 0.003 mg/mL. The RP-HPLC-PDA analysis of phenolic compounds from leaves of C. cheirifolium lets to identify: rosmarinic acid, ferulic acid, caffeic acid, p-coumaric acid, syringic acid, sinapic acid and rutin. The obtained results indicate that this plant represent a valuable source of natural antioxidants.

Oxidative Stress Modulation and ROS‐Mediated Toxicity in Cancer: A Review on In Vitro Models for Plant‐Derived Compounds

“…Another application of herbal products takes advantage of their high flavonoid content that confers them free radical scavenging properties that may, in turn, reduce cellular oxidative damage and ultimately help in the maintenance of intracellular antioxidant defenses [ 12 ]. In addition, bioactive molecules extracted from MAPs have revealed anti-inflammatory properties through TNF- α inhibition and in vitro decrease of nitric oxide generation [ 13 ].…”

HPLC-HESI-MS/MS Analysis of Phenolic Compounds from Cynoglossum tubiflorus Leaf Extracts: An Assessment of Their Cytotoxic, Antioxidant, and Antibacterial Properties

“…However, various studies have reported that Boraginaceae species are abundant in flavonoids and phenolic compounds [3,14]. Furthermore, Cynoglossum tubiflorus was found to have higher levels of phenolic and flavonoid contents compared to Cynoglossum cheirifolium L. [9] and Cynoglossum zeylanicum [10]. It exhibited double the amounts of total phenolic content (TPC) and Plants 2024, 13, 909 3 of 22 total flavonoid content (TFC).…”

“…Additionally, species like Cynoglossum cheirifolium , Cynoglossum creticum , and Cynoglossum zeylanicum have garnered scientific recognition for their antioxidant, antifungal, enzyme-inhibiting, anti-inflammatory, analgesic, and hepatoprotective properties [ 4 , 7 , 8 ]. These plants contain abundant secondary metabolites, including alkaloids, polyphenols, and fatty acids, as documented by Ilhem et al and Ranabhat et al [ 9 , 10 ]. Among these plants, Cynoglossum tubiflorus has been studied in the quest for bioactive molecules.…”