Identification and genetic structure of wild Italian Humulus lupulus L. and comparison with European and American hop cultivars using nuclear microsatellite markers

Rodolfi, Margherita; Silvanini, Annalisa; Chiancone, Benedetta; Marieschi, Matteo; Fabbri, Andrea; Bruni, Renato; Ganino, Tommaso

doi:10.1007/s10722-018-0622-9

Cited by 23 publications

(28 citation statements)

References 43 publications

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“…This result indicates the affinity of wild hops with some European cultivar selected from landraces. Other authors confirm this sentence [25,40]. Rodolfi and collaborators [25], in a population study on 123 hop genotypes, observed that Tettnanger and other European cultivars (Galena, East Kent Golding, Goldings, Challenger, H. Mittelfrüh) were clustered together with Italian wild accessions.…”

Section: Ssr Analysismentioning

confidence: 85%

“…Other authors confirm this sentence [25,40]. Rodolfi and collaborators [25], in a population study on 123 hop genotypes, observed that Tettnanger and other European cultivars (Galena, East Kent Golding, Goldings, Challenger, H. Mittelfrüh) were clustered together with Italian wild accessions. This sentence is also in accord with Patzak and coworkers [38]; the authors assert that Tettnanger cultivar, selected by German landrace, have a close relationship with the wild European ancestors [40].…”

Section: Ssr Analysismentioning

confidence: 85%

“…The nuclear DNA fragments, resulted by the DNA amplification with the SSR markers, were sized by using a conservative binning approach [36] through the statistical R software (R Development Core Team 2005), which takes into account the type of replicate and compensates for the limits of the fragment resolution. The differences among the examined hops were evaluated through the genetic similarity matrix using Euclidean distance [25,37,38]. The cluster analysis and construction of the dendrogram relative to genetic distances were obtained by using the unweighted pair-group method with arithmetic mean (UPGMA) algorithm, with Genetix [39] and XLSTAT 2009 software (AddinsoftTM 1995-2009).…”

Section: Discussionmentioning

confidence: 99%

“…Instead, the genetic study allows the differentiation among genotypes that could present the same morphological or chemical characters, and allows the recognition and discrimination of genotypes grown in different conditions or plant age; DNA analyses are able to reveal variations in genomic sequence with frequencies over 1% among individuals of the same species [20]. Among the molecular markers, SSRs are relevant and effective tools to determine genetic differences within plant genotypes [20][21][22][23][24][25][26][27][28]. Patzak and collaborators [23] used nine SSR makers and three STS to genetically characterize 136 hop genotypes from Czech Republic, France, Switzerland, the Caucasus Region, Canada, the U.S.A. and five world cultivars, highlighting the genetic differences between American and European genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Wild Corsican Hops and Assessment of the Performances of German Hops in Corsican Environmental Conditions through a Multidisciplinary Approach

et al. 2021

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Hops (Humulus lupulus L.) is a species that grows spontaneously in Corsica, but the characterization of this species in this territory has not yet been investigated. The main objectives of this study are to explore the features of wild hops from Corsica and to determine the effect of the island terroir on some cultivars in the first year of growth. A multidisciplinary approach consisting of the genetic analysis, morphological comparison and chemical characterization of essential oils was carried out on four wild Corsican hops and three hop cultivars grown in Tettnang, Germany and Corsica, France. The morphological and GC-MS analysis of Corsican wild hops, set cluster coastal samples apart from the one far from the coast. This dissimilarity is supported by the SSR analysis by two of the three coastal accessions. The genetics demonstrate a proximity between the European noble cultivar Tettnanger and the mountain Corsican wild hop from Corte. The morphological comparison between German hops cultivated in Tettnang and in Corsican soil, and the GC-MS characterization of their essential oils’ chemical profiles, show different features between year 0 and year +1 for each sample. This multidisciplinary approach highlights an acclimatization of hop cultivars to the Corsican terroir one year after planting.

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Section: Ssr Analysismentioning

confidence: 85%

Section: Ssr Analysismentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of Wild Corsican Hops and Assessment of the Performances of German Hops in Corsican Environmental Conditions through a Multidisciplinary Approach

et al. 2021

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“…Discussion SSR MARKER. The SSR marker is a powerful, reliable, and useful tool for molecular breeding and for assessing the genetic diversity of plants because of its codominance and ability to reveal a high number of alleles per locus (Bassil et al, 2018;El-Esawi et al, 2016;Liang et al, 2015;Liu et al, 2015;Rodolfi et al, 2018). This is the first study to assess the genetic diversity and relationships among 23 wild Chinese Vitis species/cultivars using this powerful microsatellite technique.…”

Section: Resultsmentioning

confidence: 99%

Genetic Diversity and Population Structure Analysis of Chinese Wild Grape Using Simple Sequence Repeat Markers

Li¹,

Fan²,

Zhang³

et al. 2021

J. Amer. Soc. Hort. Sci.

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Chinese wild Vitis is a useful gene source for resistance to biotic and abiotic stresses, although there is little research on its genetic diversity and structure. In this study, nine simple sequence repeat (SSR) markers were used to assess the genetic diversity and genetic structure among 100 Vitis materials. These materials included 77 indigenous accessions representing 23 of 38 wild Vitis species/cultivars in China, 18 V. vinifera cultivars, and the five North American species V. aestivalis, V. girdiana, V. monticola, V. acerifolia, and V. riparia. The SSR loci used in this study for establishing an international database (Vitis International Variety Catalogue) revealed a total of 186 alleles in 100 Vitis accessions. The mean values for the gene diversity (GD) and polymorphism information content (PIC) per locus were 0.91 and 0.90, respectively, which indicates that the discriminatory power of the markers is high. Based on the genetic distance data, the 100 Vitis accessions were divided into five primary clusters by cluster analysis, and five populations by structure analysis; these results indicate these Chinese wild grapes were more genetically close to European grapes than to North American species. In addition, the clustering patterns of most accessions correlated with the geographic distribution. An analysis of molecular variance (AMOVA) revealed that 3.28%, 3.27%, and 93.46% of the variance occurred between populations, between individuals within populations, and between individuals within the entire population, respectively. In addition, we identified three previously undescribed accessions (Wuzhi-1, MZL-5, and MZL-6) by cluster analysis. Our results reveal a high level of genetic diversity and variability in Vitis from China, which will be helpful in the use of genetic resources in future breeding programs. In addition, our study demonstrates that SSR markers are highly suitable for further genetic diversity analyses of Chinese wild grapes.

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Pseudoperonospora humuli might be an introduced species in Central Europe with low genetic diversity but high distribution potential

et al. 2021

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Identification and genetic structure of wild Italian Humulus lupulus L. and comparison with European and American hop cultivars using nuclear microsatellite markers

Cited by 23 publications

References 43 publications

Characterization of Wild Corsican Hops and Assessment of the Performances of German Hops in Corsican Environmental Conditions through a Multidisciplinary Approach

Characterization of Wild Corsican Hops and Assessment of the Performances of German Hops in Corsican Environmental Conditions through a Multidisciplinary Approach

Genetic Diversity and Population Structure Analysis of Chinese Wild Grape Using Simple Sequence Repeat Markers

Pseudoperonospora humuli might be an introduced species in Central Europe with low genetic diversity but high distribution potential

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