Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei

Zhang, Haofang; Li, Shihao; Wang, Fuxuan; Xiang, Jianhai; Li, Fuhua

doi:10.1016/j.dci.2020.103713

Cited by 19 publications

(15 citation statements)

References 34 publications

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“…These data suggest that PtLRR1 and PtLRR2 might participate in AMP regulation by inducing multiple signaling pathways. In addition, both PtLRR1 and PtLRR2 could enhance the clearance of V. parahaemolyticus, which is similar to the bacterial clearance activity found in MjLRRC1 from shrimp M. japonicus (Shi et al, 2017) and LvLRRm from Litopenaeus vannamei (Zhang et al, 2020). Therefore, PtLRR1 and PtLRR2 could play important roles in antibacterial immune reaction of swimming crab.…”

Section: Discussionsupporting

confidence: 68%

Two LRR-Only Proteins Involved in Antibacterial Defense and Prophenoloxidase System of Swimming Crab Portunus trituberculatus

Zhang

Liu²,

Guo³

et al. 2022

Front. Mar. Sci.

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The leucine-rich repeat (LRR) motif is evolutionarily conserved in many pattern recognition receptors. Compared to the reported LRR proteins with multiple functional domains, the role of LRR-only proteins merely containing LRR motifs remain largely unexplored. In this study, two LRR-only proteins, PtLRR1 and PtLRR2, were identified from the swimming crab Portunus trituberculatus. Five LRR motifs with a consensus sequence LxxLxxLxLxxNxL were found in their encoded peptides. Both PtLRR1 and PtLRR2 were dominantly expressed in the hepatopancreas and showed a time-dependent response post bacteria and virus stimulation. The recombinant PtLRR1 could bind to various PAMPs, including LPS, PGN, and GLU. PtLRR1 and PtLRR2 displayed different regulatory activities in inducing the expression of inflammation and proPO system-related genes. Knockdown of PtLRR2 led to the decreased expression of the tested cytokines and adapter, while PtLRR1 knockdown enhanced the expression of serine proteases, serine protease homologues, and proPO genes. In addition, knockdown of PtLRR1 or PtLRR2 reduced the clearance activity of Vibrio but upregulated the expression levels of AMPs and key genes of Toll, IMD, and JNK pathways. These results suggest that PtLRR1 and PtLRR2 could act as potential immune receptors and regulate antibacterial immunity in crab.

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Section: Discussionsupporting

confidence: 68%

Two LRR-Only Proteins Involved in Antibacterial Defense and Prophenoloxidase System of Swimming Crab Portunus trituberculatus

Zhang

Liu²,

Guo³

et al. 2022

Front. Mar. Sci.

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“…Insects are always harassed by a wide array of microbial challenges, e.g., pathogenic bacteria, from diets, hence, insects have evolved a strong immune system to cope with the infections [ 26 , 49 , 50 ]. The LRR serves as an important protein binding target in innate [ 51 ], and GILT mediates the degradation of endocytosed proteins and alters the immune response characters [ 52 ]. In N. pumilus , we found that LRR and GILT gene families were significantly expanded, and the genes from GILT family was also favored by positive selection.…”

Section: Discussionmentioning

confidence: 99%

Genomic insight into the scale specialization of the biological control agent Novius pumilus (Weise, 1892)

Tang

Huang

et al. 2022

BMC Genomics

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Background Members of the genus Novius Mulsant, 1846 (= Rodolia Mulsant, 1850) (Coleoptera, Coccinellidae), play important roles in the biological control of cotton cushion scale pests, especially those belonging to Icerya. Since the best-known species, the vedalia beetle Novius cardinalis (Mulsant, 1850) was introduced into California from Australia, more than a century of successful use in classical biological control, some species of Novius have begun to exhibit some field adaptations to novel but related prey species. Despite their economic importance, relatively little is known about the underlying genetic adaptations associated with their feeding habits. Knowledge of the genome sequence of Novius is a major step towards further understanding its biology and potential applications in pest control. Results We report the first high-quality genome sequence for Novius pumilus (Weise, 1892), a representative specialist of Novius. Computational Analysis of gene Family Evolution (CAFE) analysis showed that several orthogroups encoding chemosensors, digestive, and immunity-related enzymes were significantly expanded (P < 0.05) in N. pumilus compared to the published genomes of other four ladybirds. Furthermore, some of these orthogroups were under significant positive selection pressure (P < 0.05). Notably, transcriptome profiling demonstrated that many genes among the significantly expanded and positively selected orthogroups, as well as genes related to detoxification were differentially expressed, when N. pumilus feeding on the nature prey Icerya compared with the no feeding set. We speculate that these genes are vital in the Icerya adaptation of Novius species. Conclusions We report the first Novius genome thus far. In addition, we provide comprehensive transcriptomic resources for N. pumilus. The results from this study may be helpful for understanding the association of the evolution of genes related to chemosensing, digestion, detoxification and immunity with the prey adaptation of insect predators. This will provide a reference for future research and utilization of Novius in biological control programs. Moreover, understanding the possible molecular mechanisms of prey adaptation also inform mass rearing of N. pumilus and other Novius, which may benefit pest control.

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“…To the best of our knowledge, the leucine-rich repeats are protein interaction motifs with 20 to 29 residues that include a high proportion of leucine residues [ 53 ]. Most of the studies on leucine-rich repeat, motif-containing proteins were focused on the immunological response, in crustaceans, such as S. serrata [ 54 ], Penaeus monodon [ 55 ] , and Litopenaeus vannamei [ 56 ]. In other species, such as Drosophila, leucine-rich repeat, motif-containing proteins are also involved in cytoskeleton remodeling [ 57 ], cell morphogenesis [ 58 ], and segment morphogenesis [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Full-Length Transcriptome Reconstruction Reveals the Genetic Mechanisms of Eyestalk Displacement and Its Potential Implications on the Interspecific Hybrid Crab (Scylla serrata ♀ × S. paramamosain ♂)

Zhang

et al. 2022

Biology

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The lack of high-quality juvenile crabs is the greatest impediment to the growth of the mud crab (Scylla paramamosain) industry. To obtain high-quality hybrid offspring, a novel hybrid mud crab (S. serrata ♀ × S. paramamosain ♂) was successfully produced in our previous study. Meanwhile, an interesting phenomenon was discovered, that some first-generation (F1) hybrid offspring’s eyestalks were displaced during the crablet stage I. To uncover the genetic mechanism underlying eyestalk displacement and its potential implications, both single-molecule real-time (SMRT) and Illumina RNA sequencing were implemented. Using a two-step collapsing strategy, three high-quality reconstructed transcriptomes were obtained from purebred mud crabs (S. paramamosain) with normal eyestalks (SPA), hybrid crabs with normal eyestalks (NH), and hybrid crabs with displaced eyestalks (DH). In total, 37 significantly differential alternative splicing (DAS) events (17 up-regulated and 20 down-regulated) and 1475 significantly differential expressed transcripts (DETs) (492 up-regulated and 983 down-regulated) were detected in DH. The most significant DAS events and DETs were annotated as being endoplasmic reticulum chaperone BiP and leucine-rich repeat protein lrrA-like isoform X2. In addition, the top ten significant GO terms were related to the cuticle or chitin. Overall, high-quality reconstructed transcriptomes were obtained for the novel interspecific hybrid crab and provided valuable insights into the genetic mechanisms of eyestalk displacement in mud crab (Scylla spp.) crossbreeding.

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Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei

Cited by 19 publications

References 34 publications

Two LRR-Only Proteins Involved in Antibacterial Defense and Prophenoloxidase System of Swimming Crab Portunus trituberculatus

Two LRR-Only Proteins Involved in Antibacterial Defense and Prophenoloxidase System of Swimming Crab Portunus trituberculatus

Genomic insight into the scale specialization of the biological control agent Novius pumilus (Weise, 1892)

Full-Length Transcriptome Reconstruction Reveals the Genetic Mechanisms of Eyestalk Displacement and Its Potential Implications on the Interspecific Hybrid Crab (Scylla serrata ♀ × S. paramamosain ♂)

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