Identification and expression profiling of DNA methyltransferases during development and stress conditions in Solanaceae

Kumar, Rahul; Chauhan, Pankaj Kumar; Khurana, A. D.

doi:10.1007/s10142-016-0502-3

Cited by 24 publications

(16 citation statements)

References 69 publications

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“…However, the identification of an analogous number of C5-MTase genes in eggplant, potato and tomato may suggest a similar function within the three phylogenetically closer Solanaceae species. No CMT1-like homologs were revealed in the eggplant genome, as previously observed in other Solanaceae species [48]. This gene, when present, was found to be almost silent in A .…”

Section: Discussionmentioning

confidence: 58%

“…thaliana and tomato [32] (Table 1). The number of isolated C5-MTases corresponds to that identified in potato, but it is lower than the one reported in pepper (11) and tomato (8) [48]. Eggplant displays one MET1 gene, like tomato and potato, and 3 CMTs, like potato, and only 2 DRMs.…”

Section: Discussionmentioning

confidence: 78%

“…The domain pattern shows conservation across C5-MTase families in eggplant and mirrors the one of other plant species, strengthening the hypothesis of a common evolutionary origin and a conserved function (Fig 1 and S3 Fig). Differently from MET1 of other Solanaceae [48], Smel MET1 is characterized by the presence of two RFD domains instead of one, and by two BAH domain. RFD domains functions non-catalytically to target the protein towards replication foci allowing MET protein to methylate the correct residues.…”

Section: Discussionmentioning

confidence: 99%

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Identification of DNA methyltransferases and demethylases in Solanum melongena L., and their transcription dynamics during fruit development and after salt and drought stresses

et al. 2019

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DNA methylation through the activity of cytosine-5-methyltransferases (C5-MTases) and DNA demethylases plays important roles in genome protection as well as in regulating gene expression during plant development and plant response to environmental stresses. In this study, we report on a genome-wide identification of six C5-MTases (SmelMET1, SmelCMT2, SmelCMT3a, SmelCMT3b, SmelDRM2, SmelDRM3) and five demethylases (SmelDemethylase_1, SmelDemethylase_2, SmelDemethylase_3, SmelDemethylase_4, SmelDemethylase_5) in eggplant. Gene structural characteristics, chromosomal localization and phylogenetic analyses are also described. The transcript profiling of both C5-MTases and demethylases was assessed at three stages of fruit development in three eggplant commercial F1 hybrids: i.e. ‘Clara’, ‘Nite Lady’ and ‘Bella Roma’, representative of the eggplant berry phenotypic variation. The trend of activation of C5-MTases and demethylase genes varied in function of the stage of fruit development and was genotype dependent. The transcription pattern of C5MTAses and demethylases was also assessed in leaves of the F1 hybrid ‘Nite Lady’ subjected to salt and drought stresses. A marked up-regulation and down-regulation of some C5-MTases and demethylases was detected, while others did not vary in their expression profile. Our results suggest a role for both C5-MTases and demethylases during fruit development, as well as in response to abiotic stresses in eggplant, and provide a starting framework for supporting future epigenetic studies in the species.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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Identification of DNA methyltransferases and demethylases in Solanum melongena L., and their transcription dynamics during fruit development and after salt and drought stresses

et al. 2019

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“…In TGMS line of wheat, the pollen viability is dramatically decreased to complete sterility at a certain low temperature occurring during reproductive development [52,53]. Recent studies have shown that DMT genes contribute to plant stress responses and development by de novo establishment and subsequent maintenance of DNA methylation [71]. However, the transcript abundance of DMT genes in temperature-sensitive genic male sterile wheat has rarely been studied.…”

Section: Structural and Evolutionary Features Of Dmt Genes In Wheatmentioning

confidence: 99%

Genome-wide Identification and Transcriptional Characterization of Dna Methyltransferases Conferring Temperature-sensitive Male Sterility in Wheat

Feng

Liu

et al. 2020

Preprint

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Background: DNA methyltransferase (DMT) genes contribute to plant stress responses and development by de novo establishment and subsequent maintenance of DNA methylation during replication. The photoperiod or/and temperature-sensitive genic male sterile (P/TGMS) lines play an important role in hybrid seed production of wheat. However, only few studies have been reported about the effect of the DMT genes on temperature-sensitive male sterility of wheat. Although DMT genes have been researched in some plant species, the identification and analysis of DMT genes in wheat (Triticum aestivum L.) based on genome-wide levels has not been reported.Results: In this study, a detailed overview of phylogeny of 52 wheat DMT (TaDMT) genes was presented. Homoeolog retention for TaDMT genes was significantly above the average retention rate for whole-wheat genes, indicating the functional importance of many DMT homoeologs. We found that the strikingly high number of TaDMT genes mainly resulted from the significant expansion of TaDRM subfamily. Intriguingly, all 5 paralogs belonged to the wheat DRM subfamily and we speculated tandem duplications might play a crucial role in the TaDRM subfamily expansion. Through the transcriptional analysis of TaDMT genes in a TGMS line BS366 and its hybrids and its hybrids with other six fertile lines under sterile and fertile conditions, we concluded that TaCMT-D2, TaMET1-B1, and TaDRM-U6 might be involved in male sterility in BS366. Furthermore, a correlation analysis showed that TaMET1-B1 might negatively regulate the expression of TaRAFTIN1A, an important gene for pollen development, so we speculated an epigenetic regulatory mechanism underling the male sterility of BS366 by the interaction between TaMET1-B1 and TaRAFTIN1A.Conclusions: Our findings presented a detailed phylogenic overview of the DMT genes and could provide novel insights into the effects of the DMT genes on TGMS wheat.

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“…DNA methyltransferases methylate cytosine residues in DNA (Wada ). Plants possess three types of DNA methyltransferases that differ in protein structure and function (Kumar et al ). Maintenance of methylation patterns at CG requires the methyltransferase (MET) class of proteins, whereas plant‐specific chromomethylases (CMTs) are involved in maintenance at CHG and CHH (where H is either C, T or A) (Stroud et al ).…”

Section: Introductionmentioning

confidence: 99%

The C2 protein of tomato leaf curl Taiwan virus is a pathogenicity determinant that interferes with expression of host genes encoding chromomethylases

Tsai

Wei

et al. 2017

Physiologia Plantarum

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Tomato (Solanum lycopersicum) is one of the most important crops worldwide and is severely affected by geminiviruses. Tomato leaf curl Taiwan virus (ToLCTWV), belonging to the geminiviruses, was isolated in Taiwan and causes tremendous crop loss. The geminivirus-encoded C2 proteins are crucial for a successful interaction between the virus and host plants. However, the exact functions of the viral C2 protein of ToLCTWV have not been investigated. We analyzed the molecular function(s) of the C2 protein by transient or stable expression in tomato cv. Micro-Tom and Nicotiana benthamiana. Severe stunting of tomato and N. benthamiana plants infected with ToLCTWV was observed. Expression of ToLCTWV C2-green fluorescent protein (GFP) fusion protein was predominately located in the nucleus and contributed to activation of a coat protein promoter. Notably, the C2-GFP fluorescence was distributed in nuclear aggregates. Tomato and N. benthamiana plants inoculated with potato virus X (PVX)-C2 displayed chlorotic lesions and stunted growth. PVX-C2 elicited hypersensitive responses accompanied by production of reactive oxygen species in N. benthamiana plants, which suggests that the viral C2 was a potential recognition target to induce host-defense responses. In tomato and N. benthamiana, ToLCTWV C2 was found to interfere with expression of genes encoding chromomethylases. N. benthamiana plants with suppressed NbCMT3-2 expression were more susceptible to ToLCTWV infection. Transgenic N. benthamiana plants expressing the C2 protein showed decreased expression of the NbCMT3-2 gene and pNbCMT3-2::GUS (β-glucuronidase) promoter activity. C2 protein is an important pathogenicity determinant of ToLCTWV and interferes with host components involved in DNA methylation.

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Identification and expression profiling of DNA methyltransferases during development and stress conditions in Solanaceae

Cited by 24 publications

References 69 publications

Identification of DNA methyltransferases and demethylases in Solanum melongena L., and their transcription dynamics during fruit development and after salt and drought stresses

Identification of DNA methyltransferases and demethylases in Solanum melongena L., and their transcription dynamics during fruit development and after salt and drought stresses

Genome-wide Identification and Transcriptional Characterization of Dna Methyltransferases Conferring Temperature-sensitive Male Sterility in Wheat

The C2 protein of tomato leaf curl Taiwan virus is a pathogenicity determinant that interferes with expression of host genes encoding chromomethylases

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