Identification and expression profiles of fifteen delta-class glutathione S-transferase genes from a stored-product pest, Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae)

Jing, Tian‐Xing; Yuxian, WU; Li, Ting; Wei, Dandan; Smagghe, Guy; Wang, Jinjun

doi:10.1016/j.cbpb.2017.01.008

Cited by 26 publications

(16 citation statements)

References 37 publications

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“…Similarly, an exposure to cyhalothrin, fipronil, or endosulfan reduced the expression levels of two GST genes ( LdGSTe4 and LdGSTe6 ) in Leptinotarsa decemlineata Say ( Han et al 2016 ). This phenomenon was considered an adaptive homeostasis and an energy trade-off strategy, wherein the energy was focused to express the most crucial detoxification genes when the insects exposed to insecticides ( Jing et al 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Multiple Glutathione S-Transferase Genes in Heortia vitessoides (Lepidoptera: Crambidae): Identification and Expression Patterns

Cheng

Wang

Lyu

et al. 2018

Journal of Insect Science

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To elucidate the role of glutathione S-transferases (GSTs) in Heortia vitessoides Moore (Lepidoptera: Crambidae), one of the most destructive defoliating pests in Aquilaria sinensis (Lour.) Gilg (Thymelaeaceae) forests, 16 GST cDNAs were identified in the transcriptome of adult H. vitessoides. All cDNAs included a complete open reading frame and were designated HvGSTd1–HvGSTu2. A phylogenetic analysis showed that the 16 HvGSTs were classified into seven different cytosolic classes; three in delta, two in epsilon, three in omega, three in sigma, one in theta, two in zeta, and two in unclassified. The expression patterns of these HvGSTs in various larval and adult tissues, following exposure to half the lethal concentrations (LC50s) of chlorantraniliprole and beta-cypermethrin, were determined using real-time quantitative polymerase chain reaction (RT-qPCR). The expression levels of the 16 HvGSTs were found to differ among various larval and adult tissues. Furthermore, the RT-qPCR confirmed that the transcription levels of nine (HvGSTd1, HvGSTd3, HvGSTe2, HvGSTe3, HvGSTo3, HvGSTs1, HvGSTs3, HvGSTu1, and HvGSTu2) and six (HvGSTd1, HvGSTd3, HvGSTe2, HvGSTo2, HvGSTs1, and HvGSTu1) HvGST genes were significantly higher in the fourth-instar larvae following exposure to the insecticides chlorantraniliprole and beta-cypermethrin, respectively. These genes are potential candidates involved in the detoxification of these two insecticides. Further studies utilizing the RNA interference approach are required to enhance our understanding of the functions of these genes in this forest pest.

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Section: Discussionmentioning

confidence: 99%

Multiple Glutathione S-Transferase Genes in Heortia vitessoides (Lepidoptera: Crambidae): Identification and Expression Patterns

Cheng

Wang

Lyu

et al. 2018

Journal of Insect Science

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“…These results are consistent with those observed for B . dorsalis , Anopheles gambiae , Locusta migratoria manilensis , and Liposcelis entomophila (Enderlein), wherein genes exhibited developmental stage-specific expression [ 67 – 69 ]. The high expression of GSTs in the larval stage of P .…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptomic analysis of the alimentary tract of the tephritid gall fly, Procecidochares utilis

Lan

et al. 2018

PLoS ONE

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The tephritid gall fly, Procecidochares utilis, is an important obligate parasitic insect of the malignant weed Eupatorium adenophorum which biosynthesizes toxic secondary metabolites. Insect alimentary tracts secrete several enzymes that are used for detoxification, including cytochrome P450s, glutathione S-transferases, and carboxylesterases. To explore the adaptation of P. utilis to its toxic host plant, E. adenophorum at molecular level, we sequenced the transcriptome of the alimentary tract of P. utilis using Illumina sequencing. Sequencing and de novo assembly yielded 62,443 high-quality contigs with an average length of 604 bp that were further assembled into 45,985 unigenes with an average length of 674 bp and an N50 of 983 bp. Among the unigenes, 30,430 (66.17%) were annotated by alignment against the NCBI non-redundant protein (Nr) database, while 16,700 (36.32%), 16,267 (35.37%), and 11,530 (25.07%) were assigned functions using the Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Ontology (GO) databases, respectively. Using the comprehensive transcriptome data set, we manually identified several important gene families likely to be involved in the detoxification of toxic compounds including 21 unigenes within the glutathione S-transferase (GST) family, 22 unigenes within the cytochrome P450 (P450) family, and 16 unigenes within the carboxylesterase (CarE) family. Quantitative PCR was used to verify eight, six, and two genes of GSTs, P450s, and CarEs, respectively, in different P. utilis tissues and at different developmental stages. The detoxification enzyme genes were mainly expressed in the foregut and midgut. Moreover, the unigenes were higher expressed in the larvae, pupae, and 3-day adults, while they were expressed at lower levels in eggs. These transcriptomic data provide a valuable molecular resource for better understanding the function of the P. utilis alimentary canal. These identified genes could be pinpoints to address the molecular mechanisms of P. utilis interacting with toxic plant host.

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“…Recently, application of this approach was successful in discovery of several new enzymes including new (R)-selective amine transaminases (Schaetzle et al, 2011), catalyzing conversion of prochiral ketones into chiral (R)-amines ( Fig. 4), nitrile hydratase (Pei et al, 2014); vitamin D3specific hydroxylase (Ban et al, 2014), amidases (Ruan et al, 2016 andWu et al, 2016), solvent tolerant esterase (Fang et al, 2015); polyketide synthase (Deepa et al, 2017), chitinases (Lin et al, 2017); glutathione S-transferase (Jing et al, 2017); isoprene synthases (Ilmén et al, 2015), glycoside hydrolase family 3 (GH3) aryl β-glucosidases (Kudo et al, 2015) and others (Thevarajoo et al, 2015;Zhang et al, 2015 andFang et al, 2015).…”

Section: Database Mining Approach For Novel Enzymes Discoverymentioning

confidence: 99%

Recent Trends for Discovery and Enhancement of Enzyme Function: A Review

Ibrahim

El‐Dewany

2018

Egypt. J. Microbiol.

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R ECENTLY, there is a growing recognition of enzymes utilization as ecofriendly alternative for chemical catalysts in various industrial sectors. Two main approaches are used to expand the enzymes utilization in various industrial applications including (i) Searching nature for discovery of better performing novel biocatalysts and (ii) Improvement of the enzyme function and properties. The recent advances in biocatalyst discovery and engineering have led to an increase of the efficiency and functional diversityof enzymes. Therefore, enzyme application is expanding to broader industrial processes, including those which were previously restricted to the classical chemical catalysts. Furthermore, there is a significant success in developing made-to-order enzymes that meet the industrial process conditions requirements, leading to more efficient and cost-effective processes. Here I highlight state-of-the-art strategies and tools for novel enzyme discovery and function enhancement, including enzyme engineering at the molecular level through different approaches such as directed evolution, rational design, semi rational design and computational de novo enzyme design; in addition to the recently developed field, nanobiocatalysis.

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Identification and expression profiles of fifteen delta-class glutathione S-transferase genes from a stored-product pest, Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae)

Cited by 26 publications

References 37 publications

Multiple Glutathione S-Transferase Genes in Heortia vitessoides (Lepidoptera: Crambidae): Identification and Expression Patterns

Multiple Glutathione S-Transferase Genes in Heortia vitessoides (Lepidoptera: Crambidae): Identification and Expression Patterns

De novo transcriptomic analysis of the alimentary tract of the tephritid gall fly, Procecidochares utilis

Recent Trends for Discovery and Enhancement of Enzyme Function: A Review

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