Identification and Expression Analysis of Aquaporins in the Potato Psyllid, Bactericera cockerelli

Ibáñez, Freddy; Hancock, J L; Tamborindeguy, Cécilia

doi:10.1371/journal.pone.0111745

Cited by 26 publications

(32 citation statements)

References 41 publications

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“…Insect osmoregulatory func-99 tion protects against the transfer of water from body fluids down 100 the osmotic gradient to the gut, and the consequent dehydration 101 and death (Karley et al, 2005). Three classes of insect genes 102 expressed in the gut have been implicated in osmoregulation: (1) 103 a gut sucrase-transglucosidase, identified and characterized in 104 the pea aphid Acyrthosiphon pisum, which mediates the hydrolysis 105 of ingested sucrose to its constituent monosaccharides, glucose 106 and fructose, and oligosaccharide synthesis from glucose 107 (Cristofoletti et al, 2003;Price et al, 2007); (2) a gut aquaporin 108 that facilitates the flux of water down its osmotic gradient, charac-109 terized in the leafhopper Cicadella viridis (Le Caherec et al, 1996), 110 the pea aphid (Shakesby et al, 2009), the whitefly Bemisia tabaci 111 (Mathew et al, 2011), the potato/tomato psyllid B. cockerelli 112 (Ibanez et al, 2014) and the western tarnished plant bug (Lygus 113 hesperus) (Fabrick et al, 2014); and (3) sugar transporters that 114 remove monosaccharides from the gut lumen, characterized in 115 the pea aphid (Price and Gatehouse, 2014;Price et al, 2010) and 116 brown planthopper Nilaparvata lugens (Kikuta et al, 2010). 117 The focus of this study was two major phloem-feeding pests: Table S1.…”

Section: Aquaporin 23mentioning

confidence: 99%

RNA interference against gut osmoregulatory genes in phloem-feeding insects

Tzin

Yang

Jing

et al. 2015

Journal of Insect Physiology

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In planta RNAi (i.e. plants engineered to synthesize active RNAi molecules) has great potential as a strategy to control insect crop pests. This study investigated the impact of RNAi against osmoregulatory genes expressed in the gut of two phloem-feeding species, the green peach aphid Myzus persicae and the potato/tomato psyllid Bactericera cockerelli. The target genes comprising candidate gut sucrase, aquaporin and sugar transporter genes were identified by mining insect genomic and transcriptomic datasets for genes orthologous to empirically-tested osmoregulatory genes of the pea aphid Acyrthosiphon pisum. Insects feeding on plants with RNAi against the target genes exhibited elevated hemolymph osmotic pressure (a predicted effect of perturbed osmotic function) and some reduction in performance, especially offspring production in M. persicae and mortality in B. cockerelli, associated with up to 50% reduction in mean expression of the target genes. The effects were particularly pronounced for insects treated with RNAi against multiple osmoregulatory genes, i.e. combinatorial RNAi, suggesting that the partial silencing of multiple genes with related roles can yield greater functional impairment than RNAi against a single gene. These results demonstrate the potential of RNAi against osmoregulatory genes, but further advances to improve the efficacy of RNAi in phloem-feeding insects are required to achieve effective pest control.

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Section: Aquaporin 23mentioning

confidence: 99%

RNA interference against gut osmoregulatory genes in phloem-feeding insects

Tzin

Yang

Jing

et al. 2015

Journal of Insect Physiology

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“…Functional characterization of more aquaporins will facilitate studies related to the intricate mechanisms of water homeostasis and osmoregulation that are critical to insect species surviving in extremely salty, cold/hot and dry environments. Moreover, aquaporins may serve as potential targets for insect pest management (Douglas, ; Shakesby et al ., ; Mathew et al ., ; Wallace et al ., ; Cohen, ; Ibanez et al ., ; Tzin et al ., ). In the present paper, nine putative aquaporin genes were identified in Tribolium castaneum .…”

Section: Introductionmentioning

confidence: 97%

RNA interference‐mediated functional characterization of aquaporin genes in Tribolium castaneum

Yao

Meng

2017

Insect Molecular Biology

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An obvious challenge faced by most terrestrial insects is maintaining water homeostasis in an arid environment. Current research suggests aquaporins may be evolved to meet the challenge. However, up to now, this suggestion has not been verified in any insect that feeds upon solid food with mandibulate mouthparts. In the present paper, nine putative aquaporin genes [Tribolium castaneum big brain, T. castaneum Drosophila integral protein (TcDrip), T. castaneum Pyrocoelia rufa integral protein (TcPrip), T. castaneum aquaporin 12-like, T. castaneum entomoglyceroporin 1 (TcEglp1), TcEglp2, TcEglp3, TcEglp4 and TcEglp5] were identified in T. castaneum. The transcripts of the nine genes were easily detectable in the foregut, midgut, hindgut-Malpighian tubule complex, fat body and carcass (except gut and fat body). Amongst them TcDrip, TcPrip, TcEglp1, TcEglp3 and TcEglp5 were highly transcribed in the hindgut-Malpighian tubule complex; TcEglp4 was abundantly expressed in both the fat body and hindgut-Malpighian tubule complex. RNA interference (RNAi)-mediated knockdown of TcEglp3 caused a grey larval cuticle, in contrast to a smooth and bright cuticle in control larvae. Approximately 40% of the TcEglp3 RNAi larvae had their hindguts protruding from the anus; their fresh wet faeces were attached to the hindgut. Another 20% of these treated larvae did not defaecate normally; wet brown faeces were adhered to the anal area. As a result, the larval growth was inhibited and about 60% larval lethality occurred. Silencing of TcEglp4 or TcDrip exhibited similar but weaker defective phenotypes as those of the TcEglp3-silenced larvae. Therefore, Eglp3, Eglp4 and Drip may contribute to the conductance of water in the alimentary canal and Malpighian tubules in T. castaneum.

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“…cockerelli , but not AQP3 of the whitefly Be. tabaci MEAM1, was significantly enriched in the gut, relative to the whole body (Ibanez et al ., ; Table A). Taken with the apparent absence of AQP1 from the Ba.…”

Section: Resultsmentioning

confidence: 97%

“…The Ba. cockerelli protein BcGH13‐1 is a strong candidate for osmoregulatory function because it has enriched expression in the gut (Ibanez et al ., ; Tzin et al ., ), and possesses the catalytic residues and predicted signal peptide (Fig. ; Table S2).…”

Section: Resultsmentioning

confidence: 99%

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Evolutionary conservation of candidate osmoregulation genes in plant phloem sap‐feeding insects

Jing

White

Luan

et al. 2016

Insect Molecular Biology

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The high osmotic pressure generated by sugars in plant phloem sap is reduced in phloem-feeding aphids by sugar transformations and facilitated water flux in the gut. The genes mediating these osmoregulatory functions have been identified and validated empirically in the pea aphid Acyrthosiphon pisum: sucrase 1 (SUC1), a sucrase in glycoside hydrolase family 13 (GH13), and aquaporin 1 (AQP1), a member of the Drosophila integral protein (DRIP) family of aquaporins. Here, we describe molecular analysis of GH13 and AQP genes in phloem-feeding representatives of the four phloem-feeding groups: aphids (Myzus persicae), coccids (Planococcus citri), psyllids (Diaphorina citri, Bactericera cockerelli) and whiteflies (Bemisia tabaci MEAM1 and MED). A single candidate GH13-SUC gene and DRIP-AQP gene were identified in the genome/transcriptome of most insects tested by the criteria of sequence motif and gene expression in the gut. Exceptionally, the psyllid Ba. cockerelli transcriptome included a gutexpressed Pyrocoelia rufa integral protein (PRIP)-AQP, but has no DRIP-AQP transcripts, suggesting that PRIP-AQP is recruited for osmoregulatory function in this insect. This study indicates that phylogenetically related SUC and AQP genes may generally mediate osmoregulatory functions in these diverse phloem-feeding insects, and provides candidate genes for empirical validation and development as targets for osmotic disruption of pest species.

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Identification and Expression Analysis of Aquaporins in the Potato Psyllid, Bactericera cockerelli

Cited by 26 publications

References 41 publications

RNA interference against gut osmoregulatory genes in phloem-feeding insects

RNA interference against gut osmoregulatory genes in phloem-feeding insects

RNA interference‐mediated functional characterization of aquaporin genes in Tribolium castaneum

Evolutionary conservation of candidate osmoregulation genes in plant phloem sap‐feeding insects

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