Identification and characterization of pectin lyase gene as a virulence factor in Colletotrichum gloeosporioides

Chudasama, Kiran S.; Monpara, Jagdishchandra; Thaker, Vrinda S.

doi:10.1016/j.pmpp.2021.101706

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…Forty-seven secreted proteins have functional annotations or belong to known protein families. Among them, 11 secreted proteins belong to known effector families or contain domains that have been found in fungal effectors, which include cell wall integrity and the stress response component (WSC) domain (PF01822) [ 47 ], concanavalin A-like lectin (PF00457) [ 46 ], cerato-platanin (PF07249) [ 11 , 48 , 49 ], necrosis-inducing protein (NIP) (PF05630) [ 16 ], pectin lyase fold/virulence factor (PF00544) [ 50 ], ribonuclease (RNase) (PF00545, PF00445) [ 51 , 52 ], and peptidase family (PF00082, PF09286, PF05922, PF05577, PF00450) [ 24 , 28 , 53 , 54 ] ( Table S7 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Pathogenicity-Related Effector Proteins and the Role of Piwsc1 in the Virulence of Penicillium italicum on Citrus Fruits

Yang

Zhang

et al. 2022

JoF

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Blue mold caused by Penicillium italicum is one of the two major postharvest diseases of citrus fruits. The interactions of pathogens with their hosts are complicated, and virulence factors that mediate pathogenicity have not yet been identified. In present study, a prediction pipeline approach based on bioinformatics and transcriptomic data is designed to determine the effector proteins of P. italicum. Three hundred and seventy-five secreted proteins of P. italicum were identified, many of which (29.07%) were enzymes for carbohydrate utilization. Twenty-nine candidates were further analyzed and the expression patterns of 12 randomly selected candidate effector genes were monitored during the early stages of growth on PDA and infection of Navel oranges for validation. Functional analysis of a cell wall integrity-related gene Piwsc1, a core candidate, was performed by gene knockout. The deletion of Piwsc1 resulted in reduced virulence on citrus fruits, as presented by an approximate 57% reduction in the diameter of lesions. In addition, the mycelial growth rate, spore germination rate, and sporulation of ΔPiwsc1 decreased. The findings provide us with new insights to understand the pathogenesis of P. italicum and develop an effective and sustainable control method for blue mold.

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Section: Resultsmentioning

confidence: 99%

Identification of Pathogenicity-Related Effector Proteins and the Role of Piwsc1 in the Virulence of Penicillium italicum on Citrus Fruits

Yang

Zhang

et al. 2022

JoF

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“…It would be useful to include genes for virulence factors of Colletotrichum spp. such as for pectate lyase (Chudasama et al., 2021; Miyara et al., 2008).…”

Section: Discussionmentioning

confidence: 99%

The pathogenic diversity and host range of Colletotrichum spp. causing pepper spot and anthracnose of lychee (Litchi chinensis) in Australia

Anderson,

Coates,

Aitken

et al. 2024

Plant Pathology

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Lychee pepper spot, a field disease affecting lychee fruit skin, pedicels and petioles, is caused by Colletotrichum siamense, a fungal pathogen within the gloeosporioides species complex. Members of Colletotrichum from the gloeosporioides species complex and occasionally those from the acutatum species complex also cause postharvest anthracnose of lychee. Pepper spot was first described in Australia many years after anthracnose on lychee was first described, giving rise to the hypothesis that a novel species or strain within the gloeosporioides species complex causes pepper spot. In the present study, 19 isolates of Colletotrichum spp., collected from pepper spot and anthracnose symptoms on lychee fruit, representing 13 different genotypes across five species, were inoculated onto lychee fruit in the field or on detached fruit in the laboratory, to understand more about their pathogenic diversity. We found that symptoms were specific to genotype of the pathogen, as three genetically similar isolates of C. siamense consistently caused pepper spot and anthracnose, whilst other isolates caused anthracnose only. Cross‐inoculation studies on detached fruit of lychee, banana, avocado and mango also provided some evidence of host specialization in isolates of C. siamense infecting lychee in Australia. Our experiments provided further evidence that detached fruit assays cannot be used as a reliable proxy for field inoculation studies. This research confirms that C. siamense is a causal agent of both lychee pepper spot and lychee anthracnose in Australia, and Colletotrichum alienum and Colletotrichum queenslandicum are reported as causal agents of anthracnose of lychee for the first time.

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Occurrence of diverse plant growth promoting bacteria in soybean [Glycine max (L.) Merrill] root nodules and their prospective role in enhancing crop yield

Tariq,

Tahreem,

Zafar

et al. 2024

Biocatalysis and Agricultural Biotechnology

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Identification and characterization of pectin lyase gene as a virulence factor in Colletotrichum gloeosporioides

Cited by 5 publications

References 41 publications

Identification of Pathogenicity-Related Effector Proteins and the Role of Piwsc1 in the Virulence of Penicillium italicum on Citrus Fruits

Identification of Pathogenicity-Related Effector Proteins and the Role of Piwsc1 in the Virulence of Penicillium italicum on Citrus Fruits

The pathogenic diversity and host range of Colletotrichum spp. causing pepper spot and anthracnose of lychee (Litchi chinensis) in Australia

Occurrence of diverse plant growth promoting bacteria in soybean [Glycine max (L.) Merrill] root nodules and their prospective role in enhancing crop yield

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