Identification and Characterization of Nardilysin as a Novel Dimethyl H3K4-binding Protein Involved in Transcriptional Regulation

Abstract

Background: Very few proteins are known to read histone methylation in a state-specific manner, and NRDc is not known for a role in transcriptional regulation. Results: NRDc binds specifically H3K4me2 and has a role in transcriptional regulation. Conclusion: NRDc is a novel H3K4me2-binding protein.Significance: NRDc is the first identified protein that reads specifically H3K4me2.

PHD Finger Protein 2 (PHF2) Represses Ribosomal RNA Gene Transcription by Antagonizing PHF Finger Protein 8 (PHF8) and Recruiting Methyltransferase SUV39H1

“…Pull-down Assay with Biotinylated Histone Peptides-A pulldown assay with biotinylated histone H3 tail peptides (aa 1-21) containing or not containing H3K4me3 was performed essentially as described (35). For analyzing the binding of H3K4me3 in the context of full-length PHF2 proteins, the whole cell extracts derived from HeLa cells expressing the wild-type PHF2 or M20A mutant were used.…”

A Novel Histone H4 Arginine 3 Methylation-sensitive Histone H4 Binding Activity and Transcriptional Regulatory Function for Signal Recognition Particle Subunits SRP68 and SRP72

“…We have previously used this approach to identify chromatin effectors specific for methylated H3K4 or H3K9 (32,35,36). Three biotinylated H4 N-terminal tail peptides (amino acids 1-16) containing either no modification or R3me2s or R3me2a were synthesized.…”

Non-germ Line Restoration of Genomic Imprinting for a Small Subset of Imprinted Genes in Ubiquitin-like PHD and RING Finger Domain-Containing 1 (Uhrf1) Null Mouse Embryonic Stem Cells

“…ChIP-ChIP assay was performed with a chromatin immunoprecipitation kit (Cell Signaling Technology) or equivalent homemade buffers as described previously (32). Precipitated DNA was analyzed by qPCR.…”