Identification and characterization of MmORC4 and MmORC5 , two subunits of the mouse origin of replication recognition complex

Springer, Jan; Kneissl, Margot; Pütter,; Grummt, Friedrich

doi:10.1007/s004120050374

Cited by 12 publications

(11 citation statements)

References 21 publications

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“…In comparison to asynchronously growing cells (AS) the mitotic expression of Orc3 is essentially unchanged. These observations are in accordance with the finding that the ORC4 and ORC5 genes in humans (Quintana et al, 1997;Quintana et al, 1998) and in mice (Springer et al, 1999) are also transcribed at a constant level through the cell cycle. In contrast, the expression of ORC1 in humans (Saha et al, 1998) and mice (our unpublished data) fluctuates during the cell cycle.…”

Section: Resultssupporting

confidence: 82%

“…Thus in the meantime five murine homologues to the six ORC subunits known in S. cerevisiae are described (Takahara et al, 1996;Zisimopoulou et al, 1998;Springer et al, 1999). Most recently, an Orc6-related cDNA sequence of mouse was reported in the sequence database by Dean and O'Donnell (AF139659).…”

Section: Resultsmentioning

confidence: 99%

“…Although DNA sequences defining an origin of replication have not yet unequivocally been defined in mammals, mem-bers of a putative ORC complex homologous to yeast ORC1, 2, 4 and 5 have been identified so far in human (Gavin et al, 1995;Eki et al, 1996;Quintana et al, 1997;Ishiai et al, 1997) and in mouse (Takahara et al, 1996;Zisimopoulou et al, 1998;Springer et al, 1999), suggesting a general mechanism of initiation of DNA replication in eukaryotes. Also, a partial human homologue to ORC3p was described as a composition of human EST cDNA clones lacking the amino terminus (Tugal et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex

Springer¹,

Nanda

Hoehn³

et al. 1999

Cytogenet Genome Res

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A new member of the murine origin recognition complex (ORC) related to Saccharomyces cerevisiae ORC3 has been cloned. Transcription of ORC3 is not suppressed in mouse NIH3T3 fibroblasts made quiescent by serum starvation. The transcription level of the ORC3 gene is constantly high in all phases of the cell cycle. Murine ORC3 protein contains a putative nuclear localization signal and a non-basic helix-loop-helix motif. Both motifs are conserved in eukaryotes. A potential dimerization partner of ORC3p in the murine ORC complex is ORC1p which also contains an HLH motif. This HLH motif is also highly conserved in all eukaryotic ORC1 proteins. Comparison of murine ORC3p with other ORC3-related proteins shows high amino acid homology and motif conservation leading to the conclusion that ORC3p is part of the initiation machinery conserved in eukaryotes. The mouse ORC3 gene Orc3 was assigned to mouse chromosome 4A3 by fluorescence in situ hybridization (FISH) analysis.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex

Springer¹,

Nanda

Hoehn³

et al. 1999

Cytogenet Genome Res

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“…This indicates that transcriptional regulation of AtORC genes seems to be temporally coordinated during cell cycle progression, but not at the same stage in different AtORC genes. A comparable situation occurs in animal cells in culture where ORC transcripts are not abundant in serum-starved, quiescent cells (13,33). …”

Section: Resultsmentioning

confidence: 78%

The genes encoding Arabidopsis ORC subunits are E2F targets and the two ORC1 genes are differently expressed in proliferating and endoreplicating cells

Díaz-Triviño

Castellano

Sánchez

et al. 2005

Nucleic Acids Research

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Initiation of eukaryotic DNA replication depends on the function of pre-replication complexes (pre-RC), one of its key component being the six subunits origin recognition complex (ORC). In spite of a significant degree of conservation among ORC proteins from different eukaryotic sources, the regulation of their availability varies considerably in different model systems and cell types. Here, we show that the six ORC genes of Arabidopsis thaliana are regulated at the transcriptional level during cell cycle and development. We found that Arabidopsis ORC genes, except AtORC5, contain binding sites for the E2F family of transcription factors. Expression of AtORC genes containing E2F binding sites peaks at the G1/S-phase. Analysis of AtORC gene expression in plants with reduced E2F activity, obtained by expressing a dominant negative version of DP, the E2F heterodimerization partner, and with increased E2F activity, obtained by inactivation of the retinoblastoma protein, led us to conclude that all AtORC genes, except AtORC5 are E2F targets. Interestingly, Arabidopsis contains two AtORC1 (a and b) genes, highly conserved at the amino acid level but with unrelated promoter sequences. AtORC1b expression is restricted to proliferating cells. However, AtORC1a is preferentially expressed in endoreplicating cells based on our analysis in endoreplicating tissues and in a mutant with altered endocycle pattern. This suggests a differential expression of the two ORC1 genes in Arabidopsis.

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“…In addition, a second region of homology found in the Dbf4-like proteins of the lower eukaryotic species S. cerevisiae, S. pombe and A. nidulans could not be detected in the proteins MmDbf4p and HsDbf4p. Therefore, the strong sequence conservation commonly observed in replication proteins from dierent eukaryotic species ± examples include the Orc1, 4 and 5 proteins (Zisimopoulou et al 1998;Springer et al 1999), and Cdc7 (Jiang and Hunter 1997;Sato et al 1997;Faul et al 1999) ± is not seen in Dbf4-like proteins. In spite of the changes in the primary structure of the proteins there are strong indications for conservation of protein function (Toone et al 1997;Brown and Kelly 1998;James et al 1999).…”

Section: Resultsmentioning

confidence: 96%

Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes

et al. 1999

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The kinase Dbf4p/Cdc7p is required for the G1/S phase transition during the cell cycle and plays a direct role in the activation of individual origins of replication in Saccharomyces cerevisiae. Here, we report the identification and characterization of mouse and human cDNAs whose products are related in sequence to Saccharomyces cerevisiae DBF4 cDNA. Both mammalian Dbf4 proteins contain a putative site for phosphorylation by CDK, PEST protease cleavage sites, nuclear localization signals and a short-looped zinc finger-like domain. Transcription of MmDBF4 is suppressed in mouse NIH3T3 fibroblasts made quiescent by serum starvation. Upon replenishment of the medium, transcript levels increase during progression through G1, peaking as cells enter S phase. MmDbf4p interacts physically with Cdc7p and Mcm2p in vivo. Using fluorescence in situ hybridization (FISH), the human DBF4 gene was localized to chromosome 7 (q21.3), whereas FISH mapped the murine counterpart to band A2 on chromosome 5. The results of chromosome mapping indicate that in both mouse and human the gene is present as a single copy. The structural conservation between Dbf4-related proteins suggests that these proteins play a key role in the regulation of DNA replication during the cell cycle in all eukaryotes.

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Identification and characterization of MmORC4 and MmORC5 , two subunits of the mouse origin of replication recognition complex

Cited by 12 publications

References 21 publications

Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex

Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex

The genes encoding Arabidopsis ORC subunits are E2F targets and the two ORC1 genes are differently expressed in proliferating and endoreplicating cells

Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes

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